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Information on EC 3.6.4.B8 - clamp-loader complex and Organism(s) Escherichia coli and UniProt Accession P28631

for references in articles please use BRENDA:EC3.6.4.B8
preliminary BRENDA-supplied EC number
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Escherichia coli
UNIPROT: P28631 not found.
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota
Reaction Schemes
Synonyms
origin recognition complex, rad17, replication factor c, clamp loader, cell cycle checkpoint protein, ctf18-rfc, clamp loader complex, rfc complex, clamp-loader complex, elg1-rfc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP-dependent clamp loader complex
-
gamma clamp loader complex
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunits delta, delta', gamma/tau, chi, and psi
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the clamp loader complex is a member of the AAA+ family of ATPases (adenosine 5'-triphosphatases)
physiological function
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heptamer
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of crystal structure of mini-delta taken from a structure of the mini-delta:beta-clamp I272A/L273A complex (PDB ID 1JQL) and of the X-ray crystal structures of the gamma clamp loader complex
determination and analysis of the solution structure of the complete seven subunit clamp loader complex using small angle X-ray scattering (SAXS), modeling, detailed overview. The C-terminal regions of the gamma subunits (residues 374-431) are truncated in the crystal structures because of the flexibility of this region
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant clamp loader complex subunits delta, gamma, delta', chi, and psi
recombinant His-tagged mini-delta from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His tag by thrombin, dialysis, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene holA gene encodes the N-terminal domain (residues 1-140) of the Escherichia coli gamma clamp loader, recombinant His-tagged mini-delta is obtained by expression in Escherichia coli strain BL21(DE3)
genes dnaX, and holA-D
recombinant expression of clamp loader complex subunits delta, gamma, delta', chi, and psi
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Alyami, E.M.; Rizzo, A.A.; Beuning, P.J.; Korzhnev, D.M.
NMR resonance assignments for the N-terminal domain of the delta subunit of the E. coli gamma clamp loader complex
Biomol. NMR Assign.
11
169-173
2017
Escherichia coli (P28630 AND P28631 AND P06710 AND P28905 AND P28632), Escherichia coli
Manually annotated by BRENDA team
Oakley, A.J.
A structural view of bacterial DNA replication
Protein Sci.
28
990-1004
2019
Escherichia coli (P28630 AND P28631 AND P06710 AND P28905 AND P28632), Escherichia coli
Manually annotated by BRENDA team
Tondnevis, F.; Gillilan, R.E.; Bloom, L.B.; McKenna, R.
Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic psichi heterodimer
Struct. Dyn.
2
54701
2015
Escherichia coli (P28630 AND P28631 AND P06710 AND P28905 AND P28632), Escherichia coli
Manually annotated by BRENDA team