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Information on EC 3.6.1.9 - nucleotide diphosphatase and Organism(s) Rattus norvegicus and UniProt Accession Q924C3

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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.9 nucleotide diphosphatase
IUBMB Comments
The enzyme preferentially hydrolyses ATP, but can also hydrolyse other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates. In vitro the enzyme also acts as a nucleotidohydrolase on ADP, NAD+, NADP+, FAD, and CoA.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q924C3
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
npp, cd203c, itpase, inosine triphosphatase, inosine triphosphate pyrophosphatase, maf protein, enpp3, nppase, e-npp, nucleoside triphosphate pyrophosphohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E-NPP1
ecto-nucleotide pyrophosphatase/phosphodiesterases
-
ectonucleotide pyrophosphatase/phosphodiesterase
-
nucleotide pyrophosphatase/phosphodiesterase 1
-
E-NPP3
ecto-nucleotide pyrophosphatase/phosphodiesterase
-
-
ecto-nucleotide pyrophosphatase/phosphodiesterases
-
ectonucleotide pyrophosphatase/phosphodiesterase
NPP 1
-
isoform
NPP 2
-
isoform
NPP 3
-
isoform
nucleoside triphosphate diphosphatase
-
-
-
-
nucleoside-triphosphate pyrophosphatase
-
-
-
-
nucleotide pyrophosphatase
-
-
-
-
nucleotide pyrophosphatase-5
-
-
nucleotide pyrophosphatase/phosphodiesterase
-
-
nucleotide pyrophosphatase/phosphodiesterases
-
-
nucleotide pyrophosphohydrolase
-
-
-
-
nucleotide-sugar pyrophosphatase
-
-
-
-
pyrophosphatase, nucleoside triphosphate
-
-
-
-
additional information
see also EC 3.1.4.1
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dinucleotide nucleotidohydrolase
The enzyme preferentially hydrolyses ATP, but can also hydrolyse other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates. In vitro the enzyme also acts as a nucleotidohydrolase on ADP, NAD+, NADP+, FAD, and CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-64-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl-5'-thymidine monophosphate + H2O
4-nitrophenol + 5'-thymidine monophosphate
show the reaction diagram
artificial substrate
-
-
?
4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
show the reaction diagram
-
-
-
?
ATP + H2O
AMP + diphosphate
show the reaction diagram
-
-
-
?
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
-
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
-
-
-
?
4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
show the reaction diagram
Ap3A + H2O
?
show the reaction diagram
-
-
-
?
ATP + H2O
AMP + diphosphate
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P3-triphosphate + H2O
5'-ADP + 5'-AMP
show the reaction diagram
NPP3
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
ATP + 5'-AMP
show the reaction diagram
NPP3
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
p4A + 5'-AMP
show the reaction diagram
NPP3
-
-
?
diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
GTP + 5'-GMP
show the reaction diagram
NPP3
-
-
?
p-nitrophenyl 5'-thymidine monophosphate + H2O
4-nitrophenol + TMP
show the reaction diagram
-
-
-
-
?
thymidine-5'-L-monophosphate p-nitrophenyl ester + H2O
thymidine 5'-monophosphate + p-nitrophenol
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
-
-
-
?
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
-
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
-
the enzyme is an oligomannosidic glycoprotein that may participate in neural cell communication
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
I NPP is not further stimulated by the addition of of crescent concentrations of Ca2+ or Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha,beta-metADP
mixed inhibition type
alpha,beta-metATP
competitive inhibition
benzoyl-ATP
competitive inhibition
2',3'-dialdehyde ATP
-
competitive inhibition
2-methylthioadenosine 5'-triphosphate
-
competitive inhibition
3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate
-
competitive inhibition
ADP
-
competitive inhibition
alpha,beta-methylene ADP
-
mixed inhibition
alpha,beta-methylene ATP
-
competitive inhibition
ATP
-
competitive inhibition
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1
L-phenylalanine
-
5 mM, 10% loss of activity
N-omega-nitro-L-arginine methyl ester
-
enzyme activity is decreased in serum and platelets from N-omega-nitro-L-arginine methyl ester -treated rats
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate Ap4A, about 85% inhibition, NPP3
suramin
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0618 - 0.108
4-nitrophenyl-5'-thymidine monophosphate
0.0618 - 0.1185
4-nitrophenyl-5'-TMP
0.0495
Ap3A
pH 8.5, NPP3
0.106
p-nitrophenyl 5'-thymidine monophosphate
-
pH 8.9, 37°C
additional information
additional information
kinetic analysis
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0096
alpha,beta-metADP
pH and temperature not specified in the publication, versus 4-nitrophenyl-5'-thymidine monophosphate
0.0022
alpha,beta-metATP
pH and temperature not specified in the publication, versus 4-nitrophenyl-5'-thymidine monophosphate
0.0205
benzoyl-ATP
pH and temperature not specified in the publication
0.0098
dialATP
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2894
-
pH 8.9, 37°C, intact platelet preparation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 8.9
-
-
8.9
-
soluble fraction
9.3
-
microsomal fraction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.5
pH 7.4: about 30% of maximal activity, pH 8.5-9.0: maximal activity, NPP3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
higher activity than in the soluble fraction
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) is a metalloenzyme that belongs to the NPP family, which comprises seven subtypes (NPP1-7)
physiological function
the enzyme is involved in various biological processes including bone mineralization, soft tissue calcification, insulin receptor signalling, cancer cell proliferation and immune modulation
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ENPP1_RAT
906
1
102942
Swiss-Prot
other Location (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
NPP1 is a homodimeric type II transmembrane glycoprotein characterized by an N-terminal transmembrane domain, two somatomedin-B-like domains, a catalytic domain and a C-terminal nuclease-like domain
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
NPP1 is a homodimeric type II transmembrane glycoprotein
glycoprotein
-
oligomannosidic glycoprotein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the activity of E-NPP is significantly enhanced after treatment with imipramine (10 mg/ml, 29% increase of activity), fluoxetine (20 mg/ml, 35% increase of activity), or moclobemide (30 mg/ml, 29% increase of activity)
the activity of E-NPP is significantly enhanced after treatment with imipramine (10 mg/ml, 29% increase of activity), fluoxetine (20 mg/ml, 35% increase of activity), or moclobemide (30 mg/ml, 29% increase of activity)
the mRNA expression in the cells of the ascitic form of Walker 256 tumor reveals transcripts for isoforms NPP2 and NPP3, whereas elevated expression of NPP3 is observed in solid tumor, after 6, 10, and 15 days of inoculation
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohe, Y.; Ohnishi, H.; Okazawa, H.; Tomizawa, K.; Kobayashi, H.; Okawa, K.; Matozaki, T.
Characterization of nucleotide pyrophosphatase-5 as an oligomannosidic glycoprotein in rat brain
Biochem. Biophys. Res. Commun.
308
719-725
2003
Rattus norvegicus
Manually annotated by BRENDA team
Vollmayer, P.; Clair, T.; Goding, J.W.; Sano, K.; Servos, J.; Zimmermann, H.
Hydrolysis of diadenosine polyphosphates by nucleotide pyrophosphatases/phosphodiesterases
Eur. J. Biochem.
270
2971-2978
2003
Homo sapiens, Rattus norvegicus (P97675)
Manually annotated by BRENDA team
Cameselle, J.C.; Agudo, A.; Canales, J.; Costas, M.J.; Fernandez, A.; Flores, A.; Garcia-Diaz, M.; Gonzalez-Santiago, S.; Lopez-Gomez, J.; Ribeiro, J.M.; Vergeles, J.M.
Nucleotide ester-forming alcoholytic activities of nucleotide pyrophosphatases: implications for practical biotransformation, enzyme mechanisms and biological function
J. Mol. Catal. B
11
469-485
2001
Rattus norvegicus, Solanum tuberosum, Crotalus durissus
-
Manually annotated by BRENDA team
Fuerstenau, C.R.; Trentin, D.d.S.; Barreto-Chaves, M.L.; Sarkis, J.J.
Ecto-nucleotide pyrophosphatase/phosphodiesterase as part of a multiple system for nucleotide hydrolysis by platelets from rats: kinetic characterization and biochemical properties
Platelets
17
84-91
2006
Rattus norvegicus
Manually annotated by BRENDA team
Fuerstenau, C.R.; Trentin, D.d.a..S.; Gossenheimer, A.N.; Ramos, D.B.; Casali, E.A.; Barreto-Chaves, M.L.; Sarkis, J.J.
Ectonucleotidase activities are altered in serum and platelets of L-NAME-treated rats
Blood Cells Mol. Dis.
41
223-229
2008
Rattus norvegicus
Manually annotated by BRENDA team
Cognato, G.d.e..P.; Czepielewski, R.S.; Sarkis, J.J.; Bogo, M.R.; Bonan, C.D.
Expression mapping of ectonucleotide pyrophosphatase/phosphodiesterase 1-3 (E-NPP1-3) in different brain structures during rat development
Int. J. Dev. Neurosci.
26
593-598
2008
Rattus norvegicus (P97675), Rattus norvegicus (Q924C3)
Manually annotated by BRENDA team
Henz, S.L.; Cognato, G.d.e..P.; Vuaden, F.C.; Bogo, M.R.; Bonan, C.D.; Sarkis, J.J.
Influence of antidepressant drugs on ecto-nucleotide pyrophosphatase/phosphodiesterases (E-NPPs) from salivary glands of rats
Arch. Oral Biol.
54
730-736
2009
Rattus norvegicus (P97675), Rattus norvegicus (Q924C3)
Manually annotated by BRENDA team
Buffon, A.; Casali, E.A.; Cardoso, V.V.; Zerbini, L.F.; Robson, S.C.; Sarkis, J.J.; Wink, M.R.
Differential expression of nucleotide pyrophosphatase/phosphodiesterases by Walker 256 mammary cancer cells in solid tumors and malignant ascites
Life Sci.
86
435-440
2010
Rattus norvegicus
Manually annotated by BRENDA team
Laketa, D.; Bjelobaba, I.; Savic, J.; Lavrnja, I.; Stojiljkovic, M.; Rakic, L.; Nedeljkovic, N.
Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum
Mol. Cell. Biochem.
339
99-106
2010
Rattus norvegicus
Manually annotated by BRENDA team
Pochmann, D.; Innocente, A.M.; Buffon, A.; Freitas Sarkis, J.J.; de Oliveira Porciuncula, L.
Biochemical characterization of an ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat cardiac soluble and microsomal fractions
J. Enzyme Inhib. Med. Chem.
27
29-36
2012
Rattus norvegicus, Rattus norvegicus Wistar
Manually annotated by BRENDA team
Lee, S.Y.; Mueller, C.E.
Nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) and its inhibitors
MedChemComm
8
823-840
2017
Mus musculus (P06802), Homo sapiens (P22413), Homo sapiens, Rattus norvegicus (Q924C3)
Manually annotated by BRENDA team