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Synonyms
npp, cd203c, itpase, inosine triphosphatase, inosine triphosphate pyrophosphatase, maf protein, enpp3, nppase, e-npp, nucleoside triphosphate pyrophosphohydrolase,
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4-nitrophenyl-5'-thymidine monophosphate + H2O
4-nitrophenol + 5'-thymidine monophosphate
artificial substrate
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4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
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ATP + H2O
AMP + diphosphate
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CTP + H2O
CMP + diphosphate
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GTP + H2O
GMP + diphosphate
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UTP + H2O
UMP + diphosphate
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4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
ATP + H2O
AMP + diphosphate
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diadenosine 5',5''-P1,P3-triphosphate + H2O
5'-ADP + 5'-AMP
NPP3
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diadenosine 5',5''-P1,P4-tetraphosphate + H2O
ATP + 5'-AMP
NPP3
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diadenosine 5',5''-P1,P5-pentaphosphate + H2O
p4A + 5'-AMP
NPP3
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diguanosine 5',5'''-P1,P4-tetraphosphate + H2O
GTP + 5'-GMP
NPP3
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p-nitrophenyl 5'-thymidine monophosphate + H2O
4-nitrophenol + TMP
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thymidine-5'-L-monophosphate p-nitrophenyl ester + H2O
thymidine 5'-monophosphate + p-nitrophenol
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additional information
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4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
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4-nitrophenyl-5'-TMP + H2O
4-nitrophenol + 5'-TMP
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?
additional information
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NPP1 hydrolyzes a wide range of phosphodiester bonds, e.g. in nucleoside triphosphates, (cyclic) dinucleotides, and nucleotide sugars yielding nucleoside 5'-monophosphates as products. Its main substrate is ATP which is cleaved to AMP and diphosphate. Purine nucleotides (ATP and GTP) are better substrates than pyrimidine nucleotides (CTP and UTP)
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additional information
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the enzyme is an oligomannosidic glycoprotein that may participate in neural cell communication
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additional information
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nucleotide ester-forming alcoholytic activity
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alpha,beta-metADP
mixed inhibition type
alpha,beta-metATP
competitive inhibition
benzoyl-ATP
competitive inhibition
2',3'-dialdehyde ATP
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competitive inhibition
2-methylthioadenosine 5'-triphosphate
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competitive inhibition
3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate
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competitive inhibition
ADP
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competitive inhibition
alpha,beta-methylene ADP
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mixed inhibition
alpha,beta-methylene ATP
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competitive inhibition
ATP
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competitive inhibition
Cibacron blue
0.1 mM, substrate Ap4A, strong inhibition, NPP1
L-phenylalanine
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5 mM, 10% loss of activity
N-omega-nitro-L-arginine methyl ester
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enzyme activity is decreased in serum and platelets from N-omega-nitro-L-arginine methyl ester -treated rats
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
0.1 mM, substrate Ap4A, about 85% inhibition, NPP3
AMP
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competitive inhibition
EDTA
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EDTA
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0.1 mM, complete inhibition
EDTA
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at 0.5 mM, EDTA greatly reduces the catalytic activity
suramin
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0.25 mM, inhibition
suramin
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a P2 receptor antagonist and an inhibitor of E-NTPDase and NPP1-2 activities
additional information
NPP3 is not affected by 0.1 mM suramin
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additional information
there is a decrease in relative expression of NPP3 in brain through rat aging
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additional information
there is a decrease in relative expression of NPP3 in brain through rat aging
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additional information
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no inhibition by gadolinium chloride, levamisole, heparin, and lysophosphatidic acid in the soluble and microsomal fractions
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Ohe, Y.; Ohnishi, H.; Okazawa, H.; Tomizawa, K.; Kobayashi, H.; Okawa, K.; Matozaki, T.
Characterization of nucleotide pyrophosphatase-5 as an oligomannosidic glycoprotein in rat brain
Biochem. Biophys. Res. Commun.
308
719-725
2003
Rattus norvegicus
brenda
Vollmayer, P.; Clair, T.; Goding, J.W.; Sano, K.; Servos, J.; Zimmermann, H.
Hydrolysis of diadenosine polyphosphates by nucleotide pyrophosphatases/phosphodiesterases
Eur. J. Biochem.
270
2971-2978
2003
Homo sapiens, Rattus norvegicus (P97675)
brenda
Cameselle, J.C.; Agudo, A.; Canales, J.; Costas, M.J.; Fernandez, A.; Flores, A.; Garcia-Diaz, M.; Gonzalez-Santiago, S.; Lopez-Gomez, J.; Ribeiro, J.M.; Vergeles, J.M.
Nucleotide ester-forming alcoholytic activities of nucleotide pyrophosphatases: implications for practical biotransformation, enzyme mechanisms and biological function
J. Mol. Catal. B
11
469-485
2001
Rattus norvegicus, Solanum tuberosum, Crotalus durissus
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brenda
Fuerstenau, C.R.; Trentin, D.d.S.; Barreto-Chaves, M.L.; Sarkis, J.J.
Ecto-nucleotide pyrophosphatase/phosphodiesterase as part of a multiple system for nucleotide hydrolysis by platelets from rats: kinetic characterization and biochemical properties
Platelets
17
84-91
2006
Rattus norvegicus
brenda
Fuerstenau, C.R.; Trentin, D.d.a..S.; Gossenheimer, A.N.; Ramos, D.B.; Casali, E.A.; Barreto-Chaves, M.L.; Sarkis, J.J.
Ectonucleotidase activities are altered in serum and platelets of L-NAME-treated rats
Blood Cells Mol. Dis.
41
223-229
2008
Rattus norvegicus
brenda
Cognato, G.d.e..P.; Czepielewski, R.S.; Sarkis, J.J.; Bogo, M.R.; Bonan, C.D.
Expression mapping of ectonucleotide pyrophosphatase/phosphodiesterase 1-3 (E-NPP1-3) in different brain structures during rat development
Int. J. Dev. Neurosci.
26
593-598
2008
Rattus norvegicus (P97675), Rattus norvegicus (Q924C3)
brenda
Henz, S.L.; Cognato, G.d.e..P.; Vuaden, F.C.; Bogo, M.R.; Bonan, C.D.; Sarkis, J.J.
Influence of antidepressant drugs on ecto-nucleotide pyrophosphatase/phosphodiesterases (E-NPPs) from salivary glands of rats
Arch. Oral Biol.
54
730-736
2009
Rattus norvegicus (P97675), Rattus norvegicus (Q924C3)
brenda
Buffon, A.; Casali, E.A.; Cardoso, V.V.; Zerbini, L.F.; Robson, S.C.; Sarkis, J.J.; Wink, M.R.
Differential expression of nucleotide pyrophosphatase/phosphodiesterases by Walker 256 mammary cancer cells in solid tumors and malignant ascites
Life Sci.
86
435-440
2010
Rattus norvegicus
brenda
Laketa, D.; Bjelobaba, I.; Savic, J.; Lavrnja, I.; Stojiljkovic, M.; Rakic, L.; Nedeljkovic, N.
Biochemical characterization of soluble nucleotide pyrophosphatase/phosphodiesterase activity in rat serum
Mol. Cell. Biochem.
339
99-106
2010
Rattus norvegicus
brenda
Pochmann, D.; Innocente, A.M.; Buffon, A.; Freitas Sarkis, J.J.; de Oliveira Porciuncula, L.
Biochemical characterization of an ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP, E.C. 3.1.4.1) from rat cardiac soluble and microsomal fractions
J. Enzyme Inhib. Med. Chem.
27
29-36
2012
Rattus norvegicus, Rattus norvegicus Wistar
brenda
Lee, S.Y.; Mueller, C.E.
Nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) and its inhibitors
MedChemComm
8
823-840
2017
Mus musculus (P06802), Homo sapiens (P22413), Homo sapiens, Rattus norvegicus (Q924C3)
brenda