We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-dCTP and 8-hydroxy-dGTP.
The enzyme appears in viruses and cellular organisms
Synonyms
(d)CTP diphosphohydrolase, (d)CTP pyrophosphohydrolase,
nudG , Orf135,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(d)CTP diphosphohydrolase
-
-
-
-
(d)CTP pyrophosphohydrolase
-
-
-
-
nudG
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CTP + H2O = CMP + diphosphate
(1)
-
-
-
dCTP + H2O = dCMP + diphosphate
(2)
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(deoxy)cytidine 5'-triphosphate diphosphohydrolase
The enzyme, characterized from the bacterium Escherichia coli, is specific for the pyrimidine nucleotides CTP and dCTP. It also acts on 5-methyl-dCTP, 5-hydroxy-dCTP and 8-hydroxy-dGTP.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-hydroxy-dATP + H2O
2-hydroxy-AMP + diphosphate
5-hydroxy-CTP + H2O
5-hydroxy-CMP + diphosphate
-
-
-
?
5-hydroxy-dCTP + H2O
5-hydroxy-dCMP + diphosphate
-
4.3% of the activity with 5-hydroxy-CTP
-
?
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
8-hydroxy-dGTP + H2O
8-hydroxy-dGMP + diphosphate
arabinosyl-CTP + H2O
arabinosyl-CMP + diphosphate
-
3% of the activity with 5-methyl-dCTP
-
-
?
CDP + H2O
cytidine + diphosphate
-
2% of the activity with 5-methyl-dCTP
-
-
?
CTP + H2O
CMP + diphosphate
dCTP + H2O
dCMP + diphosphate
dTTP + H2O
dTMP + diphosphate
-
2% of the activity with 5-methyl-dCTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
1% of the activity with 5-methyl-dCTP
-
-
?
additional information
?
-
2-hydroxy-dATP + H2O
2-hydroxy-AMP + diphosphate
-
-
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-AMP + diphosphate
-
-
-
-
?
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
-
-
-
-
?
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
-
-
-
-
?
8-hydroxy-dGTP + H2O
8-hydroxy-dGMP + diphosphate
-
-
-
-
?
8-hydroxy-dGTP + H2O
8-hydroxy-dGMP + diphosphate
-
-
-
-
?
CTP + H2O
CMP + diphosphate
-
-
-
-
?
CTP + H2O
CMP + diphosphate
-
-
-
?
CTP + H2O
CMP + diphosphate
-
48% of the activity with 5-methyl-dCTP
-
-
?
dCTP + H2O
dCMP + diphosphate
-
-
-
-
?
dCTP + H2O
dCMP + diphosphate
-
-
-
?
dCTP + H2O
dCMP + diphosphate
-
23% of the activity with 5-methyl-dCTP
-
-
?
dCTP + H2O
dCMP + diphosphate
-
-
-
-
?
additional information
?
-
-
enzyme is highly specific for pyrimidine (deoxy)nucleoside triphosphates. Like other Nudix hydrolases, the enzyme cleaves its substrates to produce a nucleoside monophosphate and diphosphate
-
-
-
additional information
?
-
-
no substrates: CMP, dCMP, dATP, ATP, dGTP, GTP, dUTP, CDP-ethanolamine, CDP-choline, CDP-glucose, CDP-glycerol
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mn2+
-
about 10% of the activity with Mg2+
additional information
-
absolute requirement for a divalnt cation. No activity in presence of Zn2+, Co2+, Ca2+
Mg2+
-
required, activity constant 1.2 mM
Mg2+
-
most effective cation, with optimal activity at 5 mM Mg2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0055 - 0.31
2-hydroxy-dATP
0.0557
5-hydroxy-CTP
-
pH 8.5, 30°C
0.0057 - 0.24
5-Methyl-dCTP
0.41
8-hydroxy-dGTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0055
2-hydroxy-dATP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
0.027
2-hydroxy-dATP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.067
2-hydroxy-dATP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
0.19
2-hydroxy-dATP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
0.31
2-hydroxy-dATP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
0.0057
5-Methyl-dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
0.0069
5-Methyl-dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
0.015
5-Methyl-dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
0.028
5-Methyl-dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.172
5-Methyl-dCTP
-
pH 9.0, 37°C
0.22
5-Methyl-dCTP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
0.24
5-Methyl-dCTP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
0.428
CTP
-
pH 8.5, 30°C
0.65
dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
0.767
dCTP
-
pH 9.0, 37°C
0.77
dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
0.93
dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
0.99
dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.17 - 2.7
2-hydroxy-dATP
35.1
5-hydroxy-CTP
-
pH 8.5, 30°C
0.89
8-hydroxy-dGTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.17
2-hydroxy-dATP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.45
2-hydroxy-dATP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
1.4
2-hydroxy-dATP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
1.42
2-hydroxy-dATP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
2.7
2-hydroxy-dATP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
2.8
5-Methyl-dCTP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
6.5
5-Methyl-dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
7.7
5-Methyl-dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
9.1
5-Methyl-dCTP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
11
5-Methyl-dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
35
5-Methyl-dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
51.7
5-Methyl-dCTP
-
pH 9.0, 37°C
9.28
CTP
-
pH 8.5, 30°C
2.1
dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
2.7
dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
6.3
dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
8.8
dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
630
5-hydroxy-CTP
-
pH 8.5, 30°C
2.2
8-hydroxy-dGTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
4.6
2-hydroxy-dATP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
6.4
2-hydroxy-dATP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
14
2-hydroxy-dATP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
21
2-hydroxy-dATP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
82
2-hydroxy-dATP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
13
5-Methyl-dCTP
-
mutant E33A, pH not specified in the publication, temperature not specified in the publication
38
5-Methyl-dCTP
-
mutant D118A, pH not specified in the publication, temperature not specified in the publication
301
5-Methyl-dCTP
-
pH 9.0, 37°C
780
5-Methyl-dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
944
5-Methyl-dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
1300
5-Methyl-dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
1400
5-Methyl-dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
21.7
CTP
-
pH 8.5, 30°C
2.3
dCTP
-
mutant D118E, pH not specified in the publication, temperature not specified in the publication
3.5
dCTP
-
mutant D118N, pH not specified in the publication, temperature not specified in the publication
6.4
dCTP
-
wild-type, pH not specified in the publication, temperature not specified in the publication
14
dCTP
-
mutant R72A, pH not specified in the publication, temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
193
-
substrate 5-methyl-dCTP, pH 9.0, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118E
-
increase in hydrolysis of substrate 2-hydroxy-dATP
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
E43A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E52A
-
complete loss of activity
E52D
-
complete loss of activity
E52Q
-
complete loss of activity
E55A
-
substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity
E55D
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 2% of wild-type activity
E55Q
-
substrate 5-methyl-dCTP, about 110% of wild-type activity, substrate dCTP, 10% of wild-type activity
E56A
-
complete loss of activity
E56D
-
complete loss of activity
E56Q
-
complete loss of activity
G36A
-
complete loss of activity
G37A
-
complete loss of activity
K38A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 10% of wild-type activity
K38R
-
substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 5% of wild-type activity
L53A
-
substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 30% of wild-type activity
R51A
-
substrate 5-methyl-dCTP, about 5% of wild-type activity, substrate dCTP, 1% of wild-type activity
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
D118A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
D118N
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
E33A
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
E33Q
-
increase in hydrolysis of substrate 2-hydroxy-dATP
-
R72A
-
impaired hydrolysis of substrate 2-hydroxy-dATP
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression as glutathione S-transferase fusion protein
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kamiya, H.; Iida, E.; Harashima, H.
Important amino acids in the phosphohydrolase module of Escherichia coli Orf135
Biochem. Biophys. Res. Commun.
323
1063-1068
2004
Escherichia coli
brenda
Iida, E.; Satou, K.; Mishima, M.; Kojima, C.; Harashima, H.; Kamiya, H.
Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein
Biochemistry
44
5683-5689
2005
Escherichia coli, Escherichia coli JD22899
brenda
Fujikawa, K.; Kasai, H.
The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein
DNA Repair
1
571-576
2002
Escherichia coli, Escherichia coli (P77788)
brenda
OHandley, S.; Dunn, C.; Bessman, M.
Orf135 from Escherichia coli is a nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP
J. Biol. Chem.
276
5421-5426
2001
Escherichia coli, Escherichia coli (P77788)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
3.6.1.65 (d)CTP diphosphatase
top
Information
