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Information on EC 3.6.1.42 - guanosine-diphosphatase and Organism(s) Kluyveromyces lactis and UniProt Accession Q9HEM6

for references in articles please use BRENDA:EC3.6.1.42
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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.42 guanosine-diphosphatase
IUBMB Comments
Also acts on UDP but not on other nucleoside diphosphates and triphosphates.
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This record set is specific for:
Kluyveromyces lactis
UNIPROT: Q9HEM6
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Word Map
The taxonomic range for the selected organisms is: Kluyveromyces lactis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
gdap1, e-ntpdase, ecto-nucleoside triphosphate diphosphohydrolase, udpase, guanosine diphosphatase, gdp1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
gda1p (GDA1 gene product)
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GDPase
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guanosine diphosphatase
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phosphatase, guanosine di-
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UDPase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP + H2O = GMP + phosphate
show the reaction diagram
also acts on UDP but not on other nucleoside diphosphates and triphosphates
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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hydrolysis of diphosphate bonds
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SYSTEMATIC NAME
IUBMB Comments
GDP phosphohydrolase
Also acts on UDP but not on other nucleoside diphosphates and triphosphates.
CAS REGISTRY NUMBER
COMMENTARY hide
98037-56-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP + H2O
GMP + phosphate
show the reaction diagram
UDP + H2O
UMP + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP + H2O
GMP + phosphate
show the reaction diagram
the enzyme's GDPase activity plays a role in guanosine nucleotide sugar transport into Golgi vesicles
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-
?
UDP + H2O
UMP + phosphate
show the reaction diagram
the enzyme's UDPase activity plays a role in uridine nucleotide sugar transport into Golgi vesicles
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
stimulates both GDPase and UDPase activity
Mn2+
stimulates both GDPase and UDPase activity, stimulates UDPase activity by 5fold compared to stimulation with Ca2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme KlGdap1
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
with the catalytic domain being lumenal
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, enzyme can complement and restore activity in Saccharomyces cerevisiae deletion mutant gda1, complementation of N-glycosylation defects
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lopez-Avalos, M.D.; Uccelletti, D.; Abeijon, C.; Hirschberg, C.B.
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles
Glycobiology
11
413-422
2001
Kluyveromyces lactis (Q9HEM6), Kluyveromyces lactis
Manually annotated by BRENDA team