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Information on EC 3.6.1.29 - bis(5'-adenosyl)-triphosphatase and Organism(s) Arabidopsis thaliana and UniProt Accession F4KEV7

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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.29 bis(5'-adenosyl)-triphosphatase
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: F4KEV7 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
fhit protein, fragile histidine triad, fragile histidine triad protein, dinucleoside triphosphatase, ap3a hydrolase, diadenosine triphosphatase, ap3aase, bis(5'-adenosyl)-triphosphatase, dinucleosidetriphosphatase, p1,p3-bis(5'-adenosyl)-triphosphate adenylohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dinucleoside triphosphatase
Q9LVM4
-
Fhit protein
Q9LVM4
-
Ap3A hydrolase
-
-
-
-
Ap3Aase
-
-
-
-
Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
-
-
-
-
diadenosine 5,5-P1,P3-triphosphatase
-
-
-
-
dinucleoside-triphosphatase
-
-
dinucleosidetriphosphatase
-
-
-
-
fragile histidine triad
-
-
Fragile histidine triad protein
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
63951-94-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
Q9LVM4
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
Q9LVM4
-
-
-
?
inosine 5'-phosphoramidate + H2O
IMP + NH3
show the reaction diagram
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
64% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
19% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
48% initial velocity of degradation
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
show the reaction diagram
-
100% initial velocity of degradation
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
show the reaction diagram
-
100% initial velocities of degradation
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
27% initial velocity of degradation
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
27% initial velocities of degradation
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
Q9LVM4
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-O-(gamma-fluoromonophosphate)
Q9LVM4
-
adenosine 5'-O-(gamma-fluorotriphosphate)
Q9LVM4
-
adenosine 5'-phosphosulfate
Q9LVM4
substrate inhibition
AMP
Q9LVM4
product inhibition
P1,P3-bis(5'-adenosyl) triphosphate
Q9LVM4
substrate inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
5'-O-[amino(hydroxy)phosphoryl]adenosine
Q9LVM4
pH 6.8, 30°C
0.003
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.26
5'-O-[amino(hydroxy)phosphoryl]adenosine
Q9LVM4
pH 6.8, 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 0.235
adenosine 5'-O-(gamma-fluoromonophosphate)
0.0071
adenosine 5'-phosphosulfate
Q9LVM4
pH 6.8, 30°C
0.14
ADP
Q9LVM4
pH 6.8, 30°C
0.113
AMP
Q9LVM4
pH 6.8, 30°C
0.145
ATP
Q9LVM4
pH 6.8, 30°C
0.0021
P1,P3-bis(5'-adenosyl) triphosphate
Q9LVM4
pH 6.8, 30°C, in presence of Mg2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Q9LVM4
relative velocities of hydrolysis with different substrates, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
Q9LVM4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Q9LVM4
assay at
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FHIT_ARATH
180
0
20400
Swiss-Prot
Mitochondrion (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene At5g58240, DNA and amino acid sequence determination and analysis
Q9LVM4
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Guranowski, A.; Wojdyla, A.M.; Pietrowska-Borek, M.; Bieganowski, P.; Khurs, E.N.; Cliff, M.J.; Blackburn, G.M.; Blaziak, D.; Stec, W.J.
Fhit proteins can also recognize substrates other than dinucleoside polyphosphates
FEBS Lett.
582
3152-3158
2008
Homo sapiens, Arabidopsis thaliana (Q9LVM4)
Manually annotated by BRENDA team
Guranowski, A.; Wojdyla, A.; Zimny, J.; Wypijewska, A.; Kowalska, J.; Lukaszewicz, M.; Jemielity, J.; Darzynkiewicz, E.; Jagiello, A.; Bieganowski, P.
Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins
New J. Chem.
34
888-893
2010
Arabidopsis thaliana, Homo sapiens, Trypanosoma brucei
-
Manually annotated by BRENDA team