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2'-deoxyadenosine 5'-phosphoimidazolide + UDP
imidazole + dA(5')p3(5')U
-
H96G mutant
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
7-benzylguanosine(5')triphospho(5')adenosine + H2O
7-benzylguanosine(5')monophosphate + 7-benzylguanosine(5')diphosphate
-
-
-
-
?
7-ethylguanosine(5')triphospho(5')adenosine + H2O
7-ethylguanosine(5')monophosphate + 7-ethylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')7-methylguanosine + H2O
7-methylguanosine(5')monophosphate + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
7-methylguanosine(5')triphospho(5')cytosine + H2O
7-methylguanosine(5')monophosphate + CDP + CMP + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
7-methylguanosine(5')triphospho(5')uracil + H2O
7-methylguanosine(5')monophosphate + UDP + UMP + 7-methylguanosine(5')diphosphate
-
-
-
-
?
adenosine 5'-phosphoimidazolide + ADP
imidazole + A(5')p3(5')A
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + ATP
imidazole + A(5')p4(5')A
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + CDP
imidazole + A(5')p3(5')C
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + GDP
imidazole + A(5')p3(5')G
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + TDP
imidazole + A(5')p3(5')T
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UDP
imidazole + A(5')p3(5')U
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UTP
imidazole + A(5')p4(5')U
-
H96G mutant
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
adenosine-5'-phospho-N-methylimidazolide + H2O
?
-
-
-
-
?
adenosine-5'-phosphoimidazolide + H2O
?
-
-
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
adenosyl-guanosine triphosphate + H2O
GDP + AMP
adenosyl-ribose triphosphate + H2O
AMP + ribose diphosphate
-
-
-
-
?
adenylylfluoride + H2O
AMP + fluoride
Q9LVM4
-
-
-
?
Ap3A + H2O
ADP + AMP
-
-
-
?
Ap4C + H2O
AMP + CTP
-
-
-
-
?
Ap4G + H2O
AMP + GTP + ATP + GMP
-
-
-
-
?
Ap4U + H2O
AMP + UTP
-
-
-
-
?
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
-
-
-
-
?
ATP + 2 H2O
AMP + 2 phosphate
-
-
-
-
?
cytosine 5'-phosphoimidazolide + UDP
imidazole + C(5')p3(5')U
-
H96G mutant
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
Dixanthosine triphosphate + H2O
?
-
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
Q9LVM4
-
-
-
?
Gp3C + H2O
GMP + CDP
-
-
-
-
?
Gp4G + H2O
GMP + GTP
-
-
-
-
?
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
-
?
GpppA + H2O
GMP + ADP + GDP + AMP
inosine 5'-phosphoramidate + H2O
IMP + NH3
m27,2'OGpppG + H2O
ppm27,2'OG + guanosine monophosphate
m2N2,7GpppG + H2O
pm2N2,7G + guanosine diphosphate
-
-
-
-
?
m3N2,N2,7GpppA + H2O
ppm3N2,N2,7G + adenosine monophosphate
-
-
-
-
?
m3N2,N2,7GpppG + H2O
ppm3N2,N2,7G + guanosine monophosphate
-
-
-
-
?
m7GpppA + H2O
N7-methyl-GMP + ADP + N7-methyl-GDP + AMP
-
-
-
-
?
m7GpppG + H2O
pm7G + guanosine diphosphate
-
-
-
-
?
m7Gpppm3N6,N6,2'OA + H2O
pm7G + ppm3N6,N6,2'OA
-
-
-
-
?
m7Gpppm7G + H2O
N7-methyl-GMP + N7-methyl-GDP
-
-
-
-
?
m7GpppmN6A + H2O
pm7G + ppmN6A
-
-
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
P1,P3-bis(5'-adenosyl)triphosphate + H2O
AMP + ADP
major substrate
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
P1-P5-bis(5'-adenosyl) pentaphosphate + H2O
adenosine monophosphate + adenosine tetraphosphate
-
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
?
-
-
-
-
?
thymidine 5'-phosphoimidazolide + UDP
imidazole + T(5')p3(5')U
-
H96G mutant
-
-
?
Up3G + H2O
GMP + UDP
-
-
-
-
?
UTP + 2 H2O
UMP + 2 phosphate
-
-
-
-
?
additional information
?
-
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
-
64% initial velocity of degradation
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
-
100% initial velocity of degradationin 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
-
96% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
-
19% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
-
25% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
-
97% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
-
48% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
-
65% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
-
100% initial velocity of degradation
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
-
-
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
preferred substrate
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
-
-
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
-
preferred substrate
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
-
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
-
-
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
-
-
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
-
100% initial velocity of degradation
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
-
8% initial velocity of degradation
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
-
1% initial velocity of degradation
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
-
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
-
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
-
-
-
-
?
GpppA + H2O
GMP + ADP + GDP + AMP
-
-
-
-
?
GpppA + H2O
GMP + ADP + GDP + AMP
-
-
-
-
?
inosine 5'-phosphoramidate + H2O
IMP + NH3
Q9LVM4
-
-
-
?
inosine 5'-phosphoramidate + H2O
IMP + NH3
-
-
-
-
?
m27,2'OGpppG + H2O
ppm27,2'OG + guanosine monophosphate
-
-
-
-
?
m27,2'OGpppG + H2O
ppm27,2'OG + guanosine monophosphate
-
-
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
-
100% initial velocities of degradation
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
-
8% initial velocities of degradation
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
-
1% initial velocities of degradation
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
27% initial velocity of degradation
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
58% initial velocity of degradation
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
72% initial velocity of degradation
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
-
27% initial velocities of degradation
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
-
58% initial velocities of degradation
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
major substrate
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
-
72% initial velocities of degradation
-
-
?
P1-bis(5'-adenosyl) monophosphate + H2O
?
Q9LVM4
-
-
-
?
P1-bis(5'-adenosyl) monophosphate + H2O
?
Q9LVM4
requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
hydrolysis of Ap3A
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
highest specificity
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
highest specificity
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
-
-
-
-
?
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
-
-
-
-
?
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
-
-
-
-
?
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
Q9LVM4
the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
?
additional information
?
-
-
Fhit is a tumor suppressor protein
-
-
?
additional information
?
-
-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
?
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
?
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
-
the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze Cap 4 m7Gpppm3N6,N6,2'O, Apm2'OApm2'OCpm2N3,2'OU, m7GpppApApCpU, m7GpCH2ppG, and m7Gpppm3N6,N6,2'OApm29OA
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze Cap 4 m7Gpppm3N6,N6,2'O, Apm2'OApm2'OCpm2N3,2'OU, m7GpppApApCpU, m7GpCH2ppG, and m7Gpppm3N6,N6,2'OApm29OA
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
adenosine 5'-phosphoramidate + H2O
AMP + NH3
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
adenylylfluoride + H2O
AMP + fluoride
Q9LVM4
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
Q9LVM4
requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
additional information
?
-
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
-
-
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
-
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
-
Fhit is a tumor suppressor protein
-
-
?
additional information
?
-
-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
?
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
?
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
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-
?
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0.003 - 0.015
5'-O-[amino(hydroxy)phosphoryl]adenosine
0.003
adenosine 5'-phosphoramidate
-
pH 6.8, 30°C
0.0053 - 0.18
adenosine-5'-phospho-N-methylimidazolide
0.0062 - 0.026
adenosine-5'-phosphoimidazolide
0.00066 - 0.0016
P1,P3-bis(5'-adenosyl)triphosphate
0.0029 - 0.026
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
0.001 - 0.015
P1-P3-bis(5'-adenosyl) triphosphate
0.002 - 0.04
P1-P3-bis(5'-guanosyl) triphosphate
0.025
P1-P3-bis(5'-uracyl) triphosphate
-
-
20
UDP
-
pH 7, synthesis of Ap3U by H96G mutant
additional information
additional information
steady state kinetics of the phosphorylated enzyme form
-
0.003
5'-O-[amino(hydroxy)phosphoryl]adenosine
Q9LVM4
pH 6.8, 30°C
0.003
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
0.003
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
0.015
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
0.0053
adenosine-5'-phospho-N-methylimidazolide
-
wild-type enzyme, pH 6.6
0.18
adenosine-5'-phospho-N-methylimidazolide
-
mutant H96G, pH 6.6
0.0062
adenosine-5'-phosphoimidazolide
-
wild-type enzyme, pH 5.8
0.026
adenosine-5'-phosphoimidazolide
-
mutant H96G, pH 5.8
0.0006
Ap4A
-
-
0.00066
P1,P3-bis(5'-adenosyl)triphosphate
diphosphorylated recombinant enzyme, pH 6.8, 37°C
0.00067
P1,P3-bis(5'-adenosyl)triphosphate
monophosphorylated recombinant enzyme, pH 6.8, 37°C
0.0014
P1,P3-bis(5'-adenosyl)triphosphate
-
pH 7.0, 37°C
0.0016
P1,P3-bis(5'-adenosyl)triphosphate
nonphosphorylated recombinant enzyme, pH 6.8, 37°C
0.0029
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
wild-type enzyme, pH 7.3
0.026
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
mutant H96G, pH 8.9
0.001
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.001
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.001
P1-P3-bis(5'-adenosyl) triphosphate
-
0.0013
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.0015
P1-P3-bis(5'-adenosyl) triphosphate
-
Ap3Aase-alpha and -c
0.0019
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.0035
P1-P3-bis(5'-adenosyl) triphosphate
-
Ap3Aase-beta
0.0054
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.007
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.012
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.012
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.014
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.015
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.002
P1-P3-bis(5'-guanosyl) triphosphate
-
-
0.01
P1-P3-bis(5'-guanosyl) triphosphate
-
-
0.04
P1-P3-bis(5'-guanosyl) triphosphate
-
-
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Jakubowski, H.; Guranowski, A.
Enzymes hydrolyzing ApppA and/or AppppA in higher plants. Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds
J. Biol. Chem.
258
9982-9989
1983
Lupinus luteus
brenda
Luthje, J.; Ogilvie, A.
Catabolism of Ap3A and Ap4A in human plasma. Purification and characterization of a glycoprotein complex with 5'-nucleotide phosphodiesterase activity
Eur. J. Biochem.
149
119-127
1985
Homo sapiens
brenda
Costas, M.J.; Cameselle, J.C.; Sillero, A.
Mitochondrial location of rat liver dinucleoside triphosphatase
J. Biol. Chem.
261
2064-2067
1986
Rattus norvegicus
brenda
Gunther Sillero, M.A.; Villalba, R.; Moreno, A.; Quintanilla, M.; Lobaton, C.D.; Sillero, A.
Dinucleosidetriphosphatase from rat liver
Eur. J. Biochem.
76
331-337
1977
Rattus norvegicus
brenda
Michels, W.; Schlimme, E.
Catabolism of capped (3'-5')- and (2'-5')-adenylates in rat liver nuclei
FEBS Lett.
166
57-61
1984
Rattus norvegicus
brenda
Costas, M.J.; MOntero, J.M.; Cameselle, J.C.; Gunther Sillero, M.A.; Sillero, A.
Dinucleosidetriphosphatase from rat brain
Int. J. Biochem.
16
757-762
1984
Rattus norvegicus
brenda
Costas, M.J.; Cameselle, J.C.; Gunther Sillero, M.A.; Sillero, A.
Occurence of nucleosidetriphosphatase in the cytosol and particulate fractions from rat liver
Int. J. Biochem.
17
903-909
1985
Rattus norvegicus
brenda
Montero, J.M.; Garcia-Agndez, J.A.; Costas, M.J.; Cameselle, J.C.; Gunther Sillero, M.A.; Sillero, A.
Hydrolytic activities on diadenosine 5',5'''-P1,P3-triphosphate in the 27000 x g supernatants and precipitates from rat brain, muscle, kidney and liver
Cienc. Biol.
11
1-6
1986
Rattus norvegicus
-
brenda
Hurtado, C.; Ruiz, A.; Sillero, A.; Gunther Sillero, M.A.
Specific magnesium-dependent diadenosine 5',5'''-P1,P3-triphosphate pyrophosphohydrolase in Escherichia coli
J. Bacteriol.
169
1718-1723
1987
Escherichia coli
brenda
Barnes, L.D.; Garrison, P.N.; Siprashvili, Z.; Guranowski, A.; Robinson, A.K.; Ingram, S.W.; Croce, C.M.; Ohta, M.; Huebner, K.
Fhit, a putative tumor supressor in humans, is a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase
Biochemistry
35
11529-11535
1996
Homo sapiens
brenda
Pawelczyk, T.; Kowara, R.; Golebiowski, F.; Matecki, A.
Expression in Escherichia coli and simple purification of human Fhit protein
Protein Expr. Purif.
18
320-326
2000
Homo sapiens (P49789), Homo sapiens
brenda
Prescott, M.; Thorne, N.M.H.; Milne, A.D.; McLennan, A.G.
Characterization of a bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia
Int. J. Biochem.
24
565-571
1992
Artemia sp.
brenda
Guranowski, A.; Starzynska, E.; Gzik, L.; Langston, S.P.; Brown, P.; Blackburn, G.M.
Methylene and halomethylene analogues of diadenosine 5'5'''-P1,P3-triphosphate (ApppA) as substrates or inhibitors of ApppA-degrading enzymes
Nucleosides Nucleotides
14
731-734
1995
Lupinus luteus
-
brenda
Pace, H.C.; Garrison, P.N.; Robinson, A.K.; Barnes, L.D.; Draganescu, A.; Rsler, A.; Blackburn, G.M.; Siprashvilli, Z.; Croce, C.M.; Huebner, K.; Brenner, C.
Genetic, biochemical and crystallographic characterization of Fith-substrate complexes as the active signalling form of Fith
Proc. Natl. Acad. Sci. USA
95
5484-5489
1998
Homo sapiens
brenda
Draganescu, A.; Hodawadekar, S.C.; Gee, K.R.; Brenner, C.
Fith-nucleotide specificity probed with novel fluorescent and fluorogenic substrates
J. Biol. Chem.
275
4555-4560
2000
Homo sapiens
brenda
Brevet, A.; Chen, J.; Fromant, M.; Blanquet, S.; Plateau, P.
Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces cerevisiae
J. Bacteriol.
173
5275-5279
1991
Saccharomyces cerevisiae
brenda
Rotllan, P.; Rodriguez-Ferrer, C.R.; Asensio, A.C.; Oaknin, S.
Potent inhibition of specific diadenosine polyphosphate hydrolases by suramin
FEBS Lett.
429
143-146
1998
Rattus norvegicus
brenda
Garcia-Agndez, J.A.; Cameselle, J.C.; Costas, M.J.; Gunther Sillero, M.A.; Sillero, A.
Particulate diadenosine 5',5'''-P1,P3-triphosphate hydrolases in rat brain: two specific dinucleoside triphosphatases and two phosphodiesterase I-like hydrolases
Biochim. Biophys. Acta
1073
402-409
1991
Rattus norvegicus
brenda
Guranowski, A.; Starzynska, E.; Bojarska, E.; Stepinski, J.; Darzynkiewicz, E.
Dinucleoside 5',5'''-P1,P3-triphosphate hydrolase from yellow lupin (Lupinus luteus) seeds: purification to homogeneity and hydrolysis of mRNA 5'-cap analogs
Protein Expr. Purif.
8
416-422
1996
Lupinus luteus
brenda
Bernet, D.; Pinto, R.M.; Sillero, A.; Cameselle, J.C.
Location of dinucleoside triphosphatase in the matrix space of rat liver mitochondria
FEBS Lett.
283
286-288
1991
Rattus norvegicus
brenda
Huang, K.; Frey, P.A.
Engineering human Fhit, a diadenosine triphosphate hydrolase, into an efficient dinucleoside polyphosphate synthase
J. Am. Chem. Soc.
126
9548-9549
2004
Homo sapiens
brenda
Di Meo, G.P.; Perucatti, A.; Uboldi, C.; Roperto, S.; Incarnato, D.; Roperto, F.; Williams, J.; Eggen, A.; Ferretti, L.; Iannuzzi, L.
Comparative mapping of the fragile histidine triad (FHIT) gene in cattle, river buffalo, sheep and goat by FISH and assignment to BTA22 by RH-mapping: A comparison with HSA3
Anim. Genet.
36
363-365
2005
Bos taurus (Q4KTA6), Bos taurus
brenda
Huang, K.; Arabshahi, A.; Wei, Y.; Frey, P.A.
The mechanism of action of the fragile histidine triad, Fhit: isolation of a covalent adenylyl enzyme and chemical rescue of H96G-Fhit
Biochemistry
43
7637-7642
2004
Homo sapiens
brenda
Garrison, P.N.; Robinson, A.K.; Pekarsky, Y.; Croce, C.M.; Barnes, L.D.
Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms
Biochemistry
44
6286-6292
2005
Homo sapiens (P49789)
brenda
Asensio, A.C.; Rodriguez-Ferrer, C.R.; Oaknin, S.; Rotllan, P.
Biochemical and immunochemical characterisation of human diadenosine triphosphatase provides evidence for its identification with the tumour suppressor Fhit protein
Biochimie
88
461-471
2006
Homo sapiens, Rattus norvegicus
brenda
Nakagawa, Y.; Akao, Y.
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
Exp. Cell Res.
312
2433-2442
2006
Homo sapiens
brenda
Zhao, P.; Hou, N.; Lu, Y.
Fhit protein is preferentially expressed in the nucleus of monocyte-derived cells and its possible biological significance
Histol. Histopathol.
21
915-923
2006
Homo sapiens
brenda
Oezkara, S.K.; Corakci, A.
FHIT expression in neoplastic, hyperplastic, and normal endometrium
Int. J. Gynecol. Cancer
15
1081-1088
2005
Homo sapiens
brenda
Guranowski, A.; Wojdyla, A.M.; Pietrowska-Borek, M.; Bieganowski, P.; Khurs, E.N.; Cliff, M.J.; Blackburn, G.M.; Blaziak, D.; Stec, W.J.
Fhit proteins can also recognize substrates other than dinucleoside polyphosphates
FEBS Lett.
582
3152-3158
2008
Homo sapiens, Arabidopsis thaliana (Q9LVM4)
brenda
Mirandola, P.; Gobbi, G.; Sponzilli, I.; Malinverno, C.; Cavazzoni, A.; Alfieri, R.; Petronini, P.G.; Vitale, M.
TRAIL-induced apoptosis of FHIT-negative lung cancer cells is inhibited by FHIT re-expression
J. Cell. Physiol.
220
493-498
2009
Homo sapiens
brenda
Banerjee, H.; Palenchar, J.B.; Lukaszewicz, M.; Bojarska, E.; Stepinski, J.; Jemielity, J.; Guranowski, A.; Ng, S.; Wah, D.A.; Darzynkiewicz, E.; Bellofatto, V.
Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins
RNA
15
1554-1564
2009
Trypanosoma brucei, Trypanosoma brucei 427
brenda
Martin, J.; St-Pierre, M.V.; Dufour, J.F.
Hit proteins, mitochondria and cancer
Biochim. Biophys. Acta
1807
626-632
2011
Homo sapiens (P49789)
brenda
Krakowiak, A.; Pecherzewska, R.; Kaczmarek, R.; Tomaszewska, A.; Nawrot, B.; Stec, W.J.
Evaluation of influence of Ap4A analogues on Fhit-positive HEK293T cells; cytotoxicity and ability to induce apoptosis
Bioorg. Med. Chem.
19
5053-5060
2011
Homo sapiens (P49789)
brenda
Guranowski, A.; Wojdyla, A.; Zimny, J.; Wypijewska, A.; Kowalska, J.; Lukaszewicz, M.; Jemielity, J.; Darzynkiewicz, E.; Jagiello, A.; Bieganowski, P.
Recognition of different nucleotidyl-derivatives as substrates of reactions catalyzed by various HIT-proteins
New J. Chem.
34
888-893
2010
Arabidopsis thaliana, Homo sapiens, Trypanosoma brucei
-
brenda