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Information on EC 3.6.1.29 - bis(5'-adenosyl)-triphosphatase
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3.6.1.29 bis(5'-adenosyl)-triphosphataseSpecify your search results
Word Map
- 3.6.1.29
- carcinogenesis
-
heterozygosity
-
clinicopathological
- metastasis
-
microsatellite
- tumorigenesis
- adenocarcinoma
-
hypermethylation
-
methylation-specific
- neoplasia
-
precancerous
-
paraffin-embedded
-
intraepithelial
- papillomavirus
- polyphosphate
-
tumor-suppressor
-
preneoplastic
- p16ink4a
-
normal-sized
-
rassf1a
-
fra16d
- hippel-lindau
-
3.6.1.17
-
caretaker
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
fhit protein, fragile histidine triad, fragile histidine triad protein, dinucleoside triphosphatase, ap3a hydrolase, diadenosine triphosphatase, ap3aase, bis(5'-adenosyl)-triphosphatase, fragile hit protein, tumour suppressor fhit protein, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Ap3A hydrolase
Ap3Aase
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-
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Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
-
-
-
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diadenosine 5,5-P1,P3-triphosphatase
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-
-
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diadenosine triphosphatase
dinucleoside triphosphatase
dinucleoside-triphosphatase
dinucleosidetriphosphatase
-
-
-
-
Fhit
Fhit protein
FHIT protein/dinucleoside triphosphatase
fragile histidine triad
Fragile histidine triad protein
HIT-45 protein
Ap3A hydrolase
-
-
-
-
Fragile histidine triad protein
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
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-
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P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
the dimeric enzyme contains a catalytic histidine triad with H96 forming a covalent intermediate at the alpha-phosphate of P1,P3-bis(5'-adenosyl)triphosphate, S128, H96 and H98 are essential for the reaction, structure overview
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
two-step reaction mechanism via a fragile histidine triad Fhit-AMP intermediate and release of MgADP-, kinetic of the reaction steps, overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
KEGG
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SYSTEMATIC NAME
IUBMB Comments
P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
63951-94-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine 5'-phosphoimidazolide + UDP
imidazole + dA(5')p3(5')U
-
H96G mutant
-
-
?
7-benzylguanosine(5')triphospho(5')adenosine + H2O
7-benzylguanosine(5')monophosphate + 7-benzylguanosine(5')diphosphate
-
-
-
-
?
7-ethylguanosine(5')triphospho(5')adenosine + H2O
7-ethylguanosine(5')monophosphate + 7-ethylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')7-methylguanosine + H2O
7-methylguanosine(5')monophosphate + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
7-methylguanosine(5')triphospho(5')cytosine + H2O
7-methylguanosine(5')monophosphate + CDP + CMP + 7-methylguanosine(5')diphosphate
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-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
7-methylguanosine(5')triphospho(5')uracil + H2O
7-methylguanosine(5')monophosphate + UDP + UMP + 7-methylguanosine(5')diphosphate
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-
-
-
?
adenosine 5'-phosphoimidazolide + ADP
imidazole + A(5')p3(5')A
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H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + ATP
imidazole + A(5')p4(5')A
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H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + CDP
imidazole + A(5')p3(5')C
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H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + GDP
imidazole + A(5')p3(5')G
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + TDP
imidazole + A(5')p3(5')T
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UDP
imidazole + A(5')p3(5')U
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UTP
imidazole + A(5')p4(5')U
-
H96G mutant
-
-
?
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
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-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
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-
-
-
?
cytosine 5'-phosphoimidazolide + UDP
imidazole + C(5')p3(5')U
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H96G mutant
-
-
?
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
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-
-
-
?
P1-P5-bis(5'-adenosyl) pentaphosphate + H2O
adenosine monophosphate + adenosine tetraphosphate
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-
-
-
?
thymidine 5'-phosphoimidazolide + UDP
imidazole + T(5')p3(5')U
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H96G mutant
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-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
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64% initial velocity of degradation
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-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
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100% initial velocity of degradationin 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30°C
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-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
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96% initial velocity of degradation
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-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
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19% initial velocity of degradation
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-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
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25% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
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97% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
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48% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
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65% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
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100% initial velocity of degradation
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
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-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
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-
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
preferred substrate
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-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
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-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
reaction of EC 3.6.2.1, adenylylsulfatase
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?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
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reaction of EC 3.6.2.1, adenylylsulfatase
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?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
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100% initial velocity of degradation
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?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
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8% initial velocity of degradation
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-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
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1% initial velocity of degradation
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-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
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-
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-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
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-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
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?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
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-
-
-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
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100% initial velocities of degradation
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-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
-
8% initial velocities of degradation
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-
?
P1,P3-bis(5'-(7-methylguanosyl))triphosphate + H2O
?
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1% initial velocities of degradation
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
27% initial velocity of degradation
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-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
58% initial velocity of degradation
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?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
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72% initial velocity of degradation
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-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
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27% initial velocities of degradation
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-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
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58% initial velocities of degradation
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-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
major substrate
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-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
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72% initial velocities of degradation
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-
?
P1-bis(5'-adenosyl) monophosphate + H2O
?
Q9LVM4
requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
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highest specificity
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
highest specificity
-
-
?
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
Q9LVM4
the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
?
additional information
?
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-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
?
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
?
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
-
the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze Cap 4 m7Gpppm3N6,N6,2'O, Apm2'OApm2'OCpm2N3,2'OU, m7GpppApApCpU, m7GpCH2ppG, and m7Gpppm3N6,N6,2'OApm29OA
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze Cap 4 m7Gpppm3N6,N6,2'O, Apm2'OApm2'OCpm2N3,2'OU, m7GpppApApCpU, m7GpCH2ppG, and m7Gpppm3N6,N6,2'OApm29OA
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
P1-bis(5'-adenosyl) monophosphate + H2O
?
Q9LVM4
requirement for Mg2+
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
-
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
-
-
-
-
?
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
additional information
?
-
-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
?
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
?
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
?
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