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Information on EC 3.6.1.28 - thiamine-triphosphatase and Organism(s) Bos taurus and UniProt Accession Q8MKF1

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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.28 thiamine-triphosphatase
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This record set is specific for:
Bos taurus
UNIPROT: Q8MKF1 not found.
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The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
thiamine triphosphatase, thtpase, ttpase, thtpa, 25-kda thiamine triphosphatase, 25 kda thiamine triphosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphatase, thiamine tri-
-
-
-
-
thiamine triphosphatase
-
-
-
-
ThTPase
-
-
-
-
TTPase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoric ester hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
thiamine-triphosphate phosphohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-47-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
-
no activity
-
-
?
CTP + H2O
CDP + phosphate
show the reaction diagram
-
no activity
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
-
no activity
-
-
?
ITP + H2O
IDP + phosphate
show the reaction diagram
thiamine diphosphate + H2O
thiamine monophosphate + phosphate
show the reaction diagram
-
no activity
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
show the reaction diagram
-
-
-
-
?
UTP + H2O
UDP + phosphate
show the reaction diagram
-
no activity
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
7.3% of the activation with Mg2+
Ca2+
-
12.7% of the activation with Mg2+
Mg2+
-
absolute requirement for divalent cations, Mg2+ is most effective
Mn2+
-
30% of the activation with Mg2+
Ni2+
-
30% of the activation with Mg2+
Zn2+
-
30% of the activation with Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis-2-nitrobenzoic acid
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.039
thiamine triphosphate
-
0.1043
ITP
-
-
0.0211 - 0.0451
thiamin triphosphate
0.043 - 0.0455
thiamine triphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
240
thiamine triphosphate
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.033
-
duodenum
0.169
-
trapezius muscle
0.217
-
heart
0.22
-
lymph node
0.275
-
spleen
0.309
-
brain
0.333
-
lung
0.354
-
liver
0.377
-
kidney
648
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
-
-
8.9
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
-
pH 7.5: about 30% of maximal activity, pH 9.5: about 50% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
33 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
217 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
377 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
354 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
333 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
220 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
trapezius, 169 nmol per min per mg of wet weight
Manually annotated by BRENDA team
-
275 nmol per min per mg of wet weight
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
THTPA_BOVIN
219
0
23983
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23892
1 * 23892, mass spectrometry
25000
gel filtration
27000
1 * 27000, SDS-PAGE
26300
-
brain enzyme, gel filtration
27200
-
lung enzyme, gel filtration
27600
-
trapezius muscle enzyme, gel filtration
28300
-
kidney enzyme, gel filtration
28400
-
liver enzyme, gel filtratuon
29000
-
spleen enzyme, gel filtration
29100
31700
-
duodenum enzyme, gel filtration
33350
-
gel filtration
33900
-
1 * 33900, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
monomer
-
1 * 33900, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
not inactivated by freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M Tris-HCl buffer, stable for several months
-
4°C, stable for about 2 weeks
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Makarchikov, A.F.; Chernikevich, I.P.
Purification and characterization of thiamine triphosphatase from bovine brain
Biochim. Biophys. Acta
1117
326-332
1992
Bos taurus
Manually annotated by BRENDA team
Makarchikov, A.F.; Chernikevich, I.P.
Thiamine triphosphatase activity in bovine kidney
Biochem. Mol. Biol. Int.
46
115-123
1998
Bos taurus
Manually annotated by BRENDA team
Makarchikov, A.F.; Lakaye, B.; Gulyai, I.E.; Czerniecki, J.; Coumans, B.; Wins, P.; Grisar, T.; Bettendorff, L.
Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals
Cell. Mol. Life Sci.
60
1477-1488
2003
Bos taurus, Gallus gallus, Homo sapiens, quail, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Lakaye, B.; Makarchikov, A.F.; Antunes, A.F.; Zorzi, W.; Coumans, B.; De Pauw, E.; Wins, P.; Grisar, T.; Bettendorff, L.
Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues
J. Biol. Chem.
277
13771-13777
2002
Bos taurus (Q8MKF1), Bos taurus, Homo sapiens (Q9BU02), Homo sapiens
Manually annotated by BRENDA team