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Information on EC 3.6.1.28 - thiamine-triphosphatase and Organism(s) Rattus norvegicus and UniProt Accession Q8CGV7
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3.6.1.28 thiamine-triphosphataseSpecify your search results
Word Map
- 3.6.1.28
-
adenylyl
- electricus
- electrophorus
-
phosphohydrolases
- hydrophila
- tdp
- nitrosomonas
- diphosphatase
-
triphosphorylated
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
thiamine triphosphatase, thtpase, ttpase, thtpa, 25-kda thiamine triphosphatase, 25 kda thiamine triphosphatase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
phosphatase, thiamine tri-
-
-
-
-
thiamine triphosphatase
-
-
-
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ThTPase
TTPase
-
-
-
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ThTPase
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoric ester hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
thiamine-triphosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9068-47-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
-
-
?
ITP + H2O
IDP + phosphate
-
18% of the activity with thiamine triphosphate with liver enzyme, no activity with brain enzyme
-
-
?
thiamine diphosphate + H2O
thiamine monophosphate + phosphate
-
28% of the activity with thiamine triphosphate with liver enzyme, 6% of the activity with thiamine triphosphate with brain enzyme
-
-
?
additional information
?
-
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
-
-
?
ATP + H2O
ADP + phosphate
-
no activity with enzyme from brain and liver
-
-
?
ATP + H2O
ADP + phosphate
-
4% of the activity with thiamine triphosphate
-
-
?
CTP + H2O
CDP + phosphate
-
at 13% of the activity with thiamine triphosphate
-
-
?
CTP + H2O
CDP + phosphate
-
18% of the activity with thiamine triphosphate with liver enzyme, no activity with brain enzyme
-
-
?
GTP + H2O
GDP + phosphate
-
at 13% of the activity with thiamine triphosphate
-
-
?
GTP + H2O
GDP + phosphate
-
14% of the activity with thiamine triphosphate with liver enzyme, no activity with brain enzyme
-
-
?
GTP + H2O
GDP + phosphate
-
at 4% of the activity with thiamine triphosphate
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
-
210008, 210009, 210010, 210011, 210012, 210013, 210014, 210015, 210016, 210017, 210018, 656873, 668963
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
enzyme is involved in thiamine metabolism
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
the enzyme may play a general role in neuronal metabolism rather than a specific role in excitability. There is no apparent correlation between enzyme expression and selective vulnerability of certain brain regions to thiamine deficiency
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
thiamine triphosphate as Mg2+-thiamine triphosphate complex
-
-
?
UTP + H2O
UDP + phosphate
-
29% of the activitry with thiamine triphosphate with liver enzyme, no activity with brain enzyme
-
-
?
UTP + H2O
UDP + phosphate
-
at 2% of the activity with thiamine triphosphate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Thtpa is known to decrease the levels of the energy currency molecule, thiamine triphosphate
-
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
enzyme is involved in thiamine metabolism
-
?
thiamine triphosphate + H2O
thiamine diphosphate + phosphate
-
the enzyme may play a general role in neuronal metabolism rather than a specific role in excitability. There is no apparent correlation between enzyme expression and selective vulnerability of certain brain regions to thiamine deficiency
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Br-
-
stimulation by anions with the following order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme
Ca2+
Cl-
-
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme
I-
-
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme
Mg2+
Mn2+
NO3-
-
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme. The effect of NO3- is maximal at pH 7-8, it is negligible at pH 6.5
SCN-
-
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme
Ca2+
-
divalent cation requirement is fullfilled by Mg2+ or Ca2+ in microsomes and by Mg2+ but not Ca2+ in soluble fraction
Ca2+
-
divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+
Mg2+
-
divalent cation requirement is fullfilled by Mg2+ or Ca2+ in microsomes and by Mg2+ but not Ca2+ in soluble fraction
Mg2+
-
maximal activation occurs at a cation to substrate ratio of 1:1
Mg2+
-
divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+
Mn2+
-
divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
-
1.0 mM, 62% inhibition of membrane-associated enzyme
4-acetamide-4'-isothiocyanostilbene-2,2'-disulfonic acid
-
1.0 mM, 36% inhibition of membrane-associated enzyme
ADP
-
1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected
ATP
-
1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected
thiamine diphosphate
-
soluble enzyme, at levels of substrate 2 to 3 times Km, no inhibition of membrane-associated enzyme
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.5
thiamine triphosphate
-
membrane-associated enzyme, activated by Ca2+ or Mg2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8
-
about 55% of maximal activity at pH 5.0 and pH 8.0, membrane-associated enzyme
8 - 10
-
pH 8.0: about 60% of maximal activity, pH 10.0: about 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
-
210006, 210007, 210008, 210009, 210010, 210011, 210013, 210014, 210015, 210016, 210017, 210018, 655198
-
highest activity in hippocampal pyramidal neurons, as well as cerbellar granule cells and Purkinje cells. Some interneurons are also labeled and many thiamine triphosphatase mRNA-positive and immunoreactive cells are distributed thoughout cerebral cortical gray matter and the thalamus. No significant activity in white matter
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). THTPA_RAT
224
0
24544
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
overexpression of N-myc downstream regulated gene-1, Ndrg-1, leads to upregulation of Thtpa in cancer cells, overview
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Barchi, R.L.; Braun, P.E.
A membrane-associated thiamine triphosphatase from rat brain. Properties of the enzyme
J. Biol. Chem.
247
7668-7673
1972
Rattus norvegicus
Hashitani, Y.; Cooper, J.R.
The partial purification of thiamine triphosphatase from rat brain
J. Biol. Chem.
247
2117-2119
1972
Rattus norvegicus
Barchi, R.L.; Braun, P.E.
Thiamine triphosphatase in the nervous system
Biochim. Biophys. Acta
255
402-405
1972
Rattus norvegicus
Iwata, H.; Baba, A.; Matsuda, T.; Terashita, Z.; Ishii, K.
Role of thiamine metabolism in the central nervous system. II. Effects of various agents on thiamine triphosphatase activity in rat brain
Jpn. J. Pharmacol.
24
825-829
1974
Rattus norvegicus
Iwata, H.; Baba, A.; Matsuda, T.
Role of thiamine metabolism in the central nervous system. I. Basic properties of thiamine triphosphatase in rat brain
Jpn. J. Pharmacol.
24
817-823
1974
Rattus norvegicus
Iwata, H.; Baba, A.; Matsuda, T.; Terashita, Z.
Properties of thiamine di- and triphosphatases in rat brain microsomes: effects of chlorpromazine
J. Neurochem.
24
1209-1213
1975
Rattus norvegicus
Matsuda, T.; Tonomura, H.; Baba, A.; Iwata, H.
Membrane-associated thiamine triphosphatase in rat skeletal muscle
Int. J. Biochem.
23
1111-1114
1991
Rattus norvegicus
Barchi, R.L.; Viale, R.O.
Membrane-associated thiamin triphosphatase. II. Activation by divalent cations
J. Biol. Chem.
251
193-197
1976
Rattus norvegicus
Barchi, R.L.
Membrane thiamine triphosphatase from rat brain: inhibition by ATP and ADP
J. Neurochem.
26
715-720
1976
Rattus norvegicus
Iwata, H.; Baba, A.; Matsuda, T.; Terashita, Z.
Some properties of the enzyme system degrading phosphorylated thiamines in the brain and the effect of chlorpromazine
Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting)
2. Meeting
213-221
1974
Rattus norvegicus
-
Barchi, R.L.
Thiamine triphosphatases in brain
Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting)
195-212
1974
Rattus norvegicus
-
Penttinen, H.K.; Uotila, L.
The relation of the soluble thiamine triphosphatase activity of various rat tissues to nonspecific phosphatases
Med. Biol.
59
177-184
1981
Rattus norvegicus
Ogawa, K.; Sakai, M.
Recent findings on ultracytochemistry of thiamin phosphatases
Ann. N. Y. Acad. Sci.
378
188-214
1982
Rattus norvegicus
Makarchikov, A.F.; Lakaye, B.; Gulyai, I.E.; Czerniecki, J.; Coumans, B.; Wins, P.; Grisar, T.; Bettendorff, L.
Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals
Cell. Mol. Life Sci.
60
1477-1488
2003
Bos taurus, Gallus gallus, Homo sapiens, quail, Rattus norvegicus, Sus scrofa
Czerniecki, J.; Chanas, G.; Verlaet, M.; Bettendorff, L.; Makarchikov, A.F.; Leprince, P.; Wins, P.; Grisar, T.; Lakaye, B.
Neuronal localization of the 25-kDa specific thiamine triphosphatase in rodent brain
Neuroscience
125
833-840
2004
Rattus norvegicus
Lakaye, B.; Verlaet, M.; Dubail, J.; Czerniecki, J.; Bontems, S.; Makarchikov, A.F.; Wins, P.; Piette, J.; Grisar, T.; Bettendorff, L.
Expression of 25 kDa thiamine triphosphatase in rodent tissues using quantitative PCR and characterization of its mRNA
Int. J. Biochem. Cell Biol.
36
2032-2041
2004
Mus musculus (Q8JZL3), Mus musculus, Rattus norvegicus
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