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Information on EC 3.6.1.15 - nucleoside-triphosphate phosphatase and Organism(s) Toxoplasma gondii and UniProt Accession Q27893
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3.6.1.15 nucleoside-triphosphate phosphataseIUBMB Comments
The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. Also hydrolyses nucleoside diphosphates, thiamine diphosphate and FAD. The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin .
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Word Map
- 3.6.1.15
- adp
- ectonucleotidases
-
purinergic
- viruses
-
unwind
- 5'-nucleotidase
- potato
-
single-stranded
-
non-structural
- purine
- platelet
- virion
-
ecto-5\'-nucleotidase
- deaminase
- sars-cov-2
- coronavirus
- vaccinia
-
nucleotidase
- toxoplasma
- gondii
- apyrase
-
ecto-nucleoside
- polyproteins
- duplex
- nsp2
- ecto-atpase
-
nucleocytoplasmic
- flaviviridae
- tachyzoite
-
ecto-enzymes
- polynucleotides
-
3.6.1.5
-
mutt
-
guanylyltransferase
-
ntp-binding
- reoviridae
- primase
-
encapsidated
-
positive-stranded
- bluetongue
- polyu
- entpd1
-
phosphohydrolytic
-
replicase
- reovirus
- ssrna
- adpase
- potexvirus
-
surface-located
- medicine
-
nucleotide-mediated
The taxonomic range for the selected organisms is: Toxoplasma gondii
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
ntpase, ntpdase, nucleoside triphosphatase, ntpdase1, ntpdase2, nsp13, kidins220, nucleoside triphosphate diphosphohydrolase, nucleoside triphosphate hydrolase, pvx cp, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
NTPase-I
GP086L protein
-
-
-
-
M86L protein
-
-
-
-
MC100R
-
-
-
-
NTPase
-
-
-
-
nucleoside 5-triphosphatase
-
-
-
-
nucleoside triphosphate phosphohydrolase
-
-
-
-
nucleoside-5-triphosphate phosphohydrolase
-
-
-
-
phosphatase, nucleoside tri-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
unspecific diphosphate phosphohydrolase
The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. Also hydrolyses nucleoside diphosphates, thiamine diphosphate and FAD. The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin [5].
CAS REGISTRY NUMBER
COMMENTARY
9075-51-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
additional information
?
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
additional information
?
-
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
additional information
?
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
additional information
?
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
additional information
?
-
-
the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells
-
-
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
maintained in human foreskin fibroblast (HFF) cell monolayers in regular growth media
maintained in human foreskin fibroblast (HFF) cell monolayers in regular growth media
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. The NTPase I knockout in the type I strain mirrors the less inhibitory phenotype of type II (ME49) and III (CTG) strains, which naturally lack NTPase I
physiological function
strong negative influence of NTPase I on the FLUC activity in both HeLa cells and RAW-264.7 macrophages
malfunction
knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. In contrast to the NTPase I mutant, deletion of NTPase II causes only a mild change in inhibition of the FLUC activity in both HeLa cells and RAW-264.7 macrophages
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NTP1_TOXGO
628
0
69159
Swiss-Prot
Secretory Pathway (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 10-12% (w/v) PEG 3350, 0.1 M Tris-HCl pH 7.4, 2% (v/v) glycerol, at 20°C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. Toxoplasma gondii strains null for both NTPase I and NTPase II can be used to avoid interference artifacts in ATP-dependent assays
additional information
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. Toxoplasma gondii strains null for both NTPase I and NTPase II can be used to avoid interference artifacts in ATP-dependent assays
additional information
-
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. Toxoplasma gondii strains null for both NTPase I and NTPase II can be used to avoid interference artifacts in ATP-dependent assays
additional information
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. ATP-dependent assays
additional information
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. ATP-dependent assays
additional information
-
generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. ATP-dependent assays
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matoba, K.; Shiba, T.; Takeuchi, T.; Sibley, L.D.; Seiki, M.; Kikyo, F.; Horiuchi, T.; Asai, T.; Harada, S.
Crystallization and preliminary X-ray structural analysis of nucleoside triphosphate hydrolases from Neospora caninum and Toxoplasma gondii
Acta Crystallogr. Sect. F
66
1445-1448
2010
Neospora caninum (C9K7J7), Toxoplasma gondii (Q27893)
EXTERNAL LINKS (specific for EC-Number 3.6.1.15)
Transporter Classification Database (TCDB): 1.G.3.1.7, 1.A.53.1.5, 1.A.122.1.2, 1.A.53.1.7, 1.A.85.1.7, 1.G.3.1.2, 1.G.3.1.1, 1.A.85.1.4, 1.A.85.1.8, 1.A.53.1.11, 1.A.53.1.6, 1.A.85.1.1
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