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Information on EC 3.6.1.13 - ADP-ribose diphosphatase
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3.6.1.13 ADP-ribose diphosphataseSpecify your search results
Word Map
- 3.6.1.13
- pyrophosphatases
-
melastatin
-
mutt-related
-
ribose-5'-phosphate
- adp-sugars
- 8-oxo-dgdp
The enzyme appears in viruses and cellular organisms
Synonyms
nudt5, nudt9, adp-ribose pyrophosphatase, adprase, atnudt7, adpribase-mn, atnudx7, adp-ribose hydrolase, nudt5 protein, adpr pyrophosphatase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosine diphosphoribose pyrophosphatase
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-
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ADP-ribose diphosphatase
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ADP-ribose phosphohydrolase
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-
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ADP-ribose pyrophosphatase
ADP-ribose pyrophosphatase-I
ADP-ribose/CDP-alcohol diphosphatase
ADP-ribose/NADH pyrophosphohydrolase
ADPR pyrophosphatase
ADPR-PPase
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-
-
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ADPRase
ADPRase-I
ADPRibase
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ADPribose pyrophosphatase
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-
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AT4G12720
AtNUDX7
CinA
MT1739
MtADPRase
MutT/nudix family protein
NadM-Nudix
Ndx2
Ndx4
NMN adenylyltransferase/ADP-ribose pyrophosphatase
Nudix hydrolase 7
NUDT5
pyrophosphatase, adenosine diphosphoribose
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-
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additional information
ADP-ribose pyrophosphatase
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-
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-
additional information
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the enzyme belongs to the Nudix, nucleotide diphosphate X,pyrophosphatase superfamily
additional information
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the enzyme is a member of a superfamily of Nudix hydrolases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate
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-
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ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate
molecular catalytic mechanism involving Arg51, Arg111, Glu112, and Glu116, active site structure, substrate binding involving Arg51, Leu98, Trp28 and Trp46 and transition-state structure, structure-function relationship, and kinetic analysis
ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate
substrate binding mode in the active site of the ADPRase domain and catalytic mechanism
ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate
substrate binding mode in the active site of the ADPRase domain and catalytic mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphorous acid anhydride hydrolysis
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-
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-
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SYSTEMATIC NAME
IUBMB Comments
ADP-D-ribose ribophosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9024-83-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,Nepsilon-etheno-ADP-ribose + H2O
1,Nepsilon-etheno-AMP-ribose + phosphate
-
fluorogenic substrate
the product is converted to fluorescent 1,Nepsilon-etheno-adenosine by alkaline phosphatase for detection
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?
ADPribose 2'-phosphate + H2O
adenosine 2',5'-diphosphate + D-ribose 5-phosphate
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-
-
-
?
CDP-glucose + H2O
CMP + glucose 5-phosphate
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at 5% of the activity with ADPribose
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-
?
CDP-ribose + H2O
CMP + D-ribose 5-phosphate
-
kcat/Km is 2.5% of the kcat/Km for ADP-ribose
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-
?
diadenosine 5',5''-diphosphate + H2O
?
20% the activity with ADP-ribose
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-
?
GDP-ribose + H2O
GMP + D-ribose 5-phosphate
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kcat/Km is 3% of the kcat/Km for ADP-ribose
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-
?
IDP-ribose + H2O
IMP + D-ribose 5-phosphate
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138% of the activity with ADPribose
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-
?
IDPribose + H2O
IMP + D-ribose 5-phosphate
77% of the activity with ADPribose
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-
?
NAD+ + H2O
?
-
ADPRibase-Mn in presence of Mn2+, no activity with ADPRibase I and ADPRibase II
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-
?
UDP-galactose + H2O
UDP + galactose
7% of the activity with ADP-ribose
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-
?
ADP-alpha-D-ribose + H2O
AMP + D-ribose 5-phosphate
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-
-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
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at 21% of the activity with ADPribose
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
56% of the activity with ADP-ribose
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
about 70% of the activity with ADP-mannose
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
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about 70% of the activity with ADP-ribose
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
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ADPRibase II, very low activity with ADPRibase I
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
9% of the activity with ADPribose
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-
?
ADP-glucose + H2O
AMP + alpha-D-glucose 1-phosphate
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kcat/Km is 3% of the kcat/Km for ADP-ribose
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-
?
ADP-mannose + H2O
AMP + D-mannose 1-phosphate
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at 70% of the activity with ADPribose
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-
?
ADP-mannose + H2O
AMP + D-mannose 1-phosphate
103% of the activity with ADP-ribose
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-
?
ADP-mannose + H2O
AMP + D-mannose 1-phosphate
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about 80% of the activity with ADP-ribose
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?
ADP-mannose + H2O
AMP + D-mannose 1-phosphate
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ADPRibase II, low activity with ADPRibase I
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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Growth Factor Gene 1 (GFG1, At4g12720) encodes a nudix hydrolase, that is an NADH pyrophosphatase and ADP-ribose pyrophosphatase
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT10 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT2 shows both ADP-ribose and NADH pyrophosphatase activity
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-
?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT6 shows both ADP-ribose and NADH pyrophosphatase activity
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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AtNUDT7 shows both ADP-ribose and NADH pyrophosphatase activity
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
substrate binding structure, overview
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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the enzyme may have a predominant role in free ADP-ribose turnover and as a protective agent preventing the accumulation of this potential dangerous metabolite
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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protective enzyme whose function is to limit the intracellular accumulation of ADP-ribose
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
the enzyme plays important roles in controlling the intracellular levels of ADPR and preventing non-enzymatic ADP-ribosylation of proteins by hydrolyzing ADPR to AMP and ribose 5?-phosphate
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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ADP-ribose pyrophosphatase Sll1054 hydrolyzes ADP-ribose specifically
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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ADP-ribose pyrophosphatase Slr0920 hydrolyzes ADP-ribose specifically
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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ADP-ribose pyrophosphatase Slr1134 hydrolyzes ADP-ribose, NADH and flavin adenine dinucleotide
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
substrate binding structure, overview
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
highly specific substrate
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
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interactions responsible for the substrate recognition are located at the terminal moieties of the substrate. The adenine moiety is recognized by Ile-19 and the main chain carbonyl group of Glu-29 and/or Gly-104. The terminal ribose moiety is recognized by the sum of some weak interactions with multiple residues that are close in space. Glu-82 and Glu-86 are essential for catalysis but unlikely to act as a catalytic base. Two-metal ion mechanism for the catalysis of ADPRase in which a water molecule is activated to act as a nucleophile by the cations coordinated by Glu-82 and Glu-86. Arg-54, Glu-70, Arg-81, and Glu-85 are predicted to support this nucleophilic attack on the R-phosphate of the substrate
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
preferred substrate, substrate recognition and binding structure, overview
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
highly specific substrate
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?
ADP-ribose + H2O
AMP + D-ribose 5-phosphate
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
preferred substrate, substrate recognition and binding structure, overview
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?
ADP-ribose + H2O
AMP + ribose 5-phosphate
the function of the enzyme might be to remove free ADP-ribose arising from NAD+ and protein-bound poly- and non-enzymic protein glycation
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?
ADP-ribose + H2O
AMP + ribose 5-phosphate
about 65% of the activity with ADP-mannose
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?
ADP-ribose + H2O
AMP + ribose 5-phosphate
ADP-ribose is a better substrate compared with 8-oxo-dGDP
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?
ADPribose + H2O
AMP + D-ribose 5-phosphate
the enzyme may play a role in tellurite resistance
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?
ADPribose + H2O
AMP + D-ribose 5-phosphate
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the enzyme may play a role in tellurite resistance
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?
ADPribose + H2O
AMP + D-ribose 5-phosphate
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the enzyme may play a role in tellurite resistance
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?
ADPribose + H2O
AMP + D-ribose 5-phosphate
nucleophilic attack at the adenosyl phosphate. The enzyme cycles between an open free enzyme and a closed substrate-metal complex conformation. This cycling may be important in preventing nonspecific hydrolysis of other nucleotides
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-
?
ADPribose + H2O
AMP + D-ribose 5-phosphate
the enzyme may play a role in tellurite resistance
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?
ADPribose + H2O
AMP + D-ribose 5-phosphate
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the enzyme may play an important role in the regulation of intracellular steady-state of free ADPribose
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-
?
ADPribose + H2O
AMP + D-ribose 5-phosphate
-
the enzyme may play a role in tellurite resistance
-
-
?
ADPribose + H2O
AMP + D-ribose 5-phosphate
-
-
-
-
?
ADPribose + H2O
AMP + D-ribose 5-phosphate
-
the enzyme may play a role in tellurite resistance
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-
?
FAD + H2O
?
-
ADPRibase-Mn and ADPRibase II, very low activity with ADPRibase I in presence of Mn2+
-
-
?
GDP-glucose + H2O
GMP + glucose 5-phosphate
7% of the activity with ADP-ribose
-
-
?
GDP-glucose + H2O
GMP + glucose 5-phosphate
about 15% of the activity with ADP-mannose
-
-
?
GDP-glucose + H2O
GMP + glucose 5-phosphate
-
about 15% of the activity with ADP-ribose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
at 59% of the activity with ADPribose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
-
at 40% of the activity with ADPribose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
about 15% of the activity with ADP-mannose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
-
about 20% of the activity with ADP-ribose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
-
at 9% of the activity with ADPribose
-
-
?
GDP-mannose + H2O
GMP + D-mannose 1-phosphate
-
at 1% of the activity with ADPribose
-
-
?
IDP-ribose + H2O
IMP + ribose 5-phosphate
-
77% of the activity with ADPribose
-
-
?
IDP-ribose + H2O
IMP + ribose 5-phosphate
-
at 59% of the activity with ADPribose
-
-
?
IDP-ribose + H2O
IMP + ribose 5-phosphate
9% of the activity with ADP-ribose
-
-
?
NADH + H2O
?
-
ADPRibase I, ADPribase II and ADPRibase-Mn in presence of Mn2+
-
-
?
UDP-glucose + H2O
UMP + glucose 5-phosphate
-
at 5% of the activity with ADPribose
-
-
?
UDP-glucose + H2O
UMP + glucose 5-phosphate
about 12% of the activity with ADP-mannose
-
-
?
UDP-glucose + H2O
UMP + glucose 5-phosphate
-
about 5% of the activity with ADP-ribose
-
-
?
UDP-mannose + H2O
UMP + D-mannose 1-phosphate
-
at 20% of the activity with ADPribose
-
-
?
UDP-mannose + H2O
UMP + D-mannose 1-phosphate
about 15% of the activity with ADP-mannose