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Information on EC 3.6.1.1 - inorganic diphosphatase and Organism(s) Pyrococcus furiosus and UniProt Accession Q8U438

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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.1 inorganic diphosphatase
IUBMB Comments
Specificity varies with the source and with the activating metal ion. The enzyme from some sources may be identical with EC 3.1.3.1 (alkaline phosphatase) or EC 3.1.3.9 (glucose-6-phosphatase). cf. EC 7.1.3.1, H+-exporting diphosphatase.
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This record set is specific for:
Pyrococcus furiosus
UNIPROT: Q8U438
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The taxonomic range for the selected organisms is: Pyrococcus furiosus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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diphosphate
+
H2O
=
2
phosphate
+
=
2
Synonyms
pyrophosphatase, inorganic pyrophosphatase, v-ppase, h+-ppase, vacuolar h(+)-pyrophosphatase, sppase, e-ppase, vacuolar h(+)-ppase, soluble inorganic pyrophosphatase, ippase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
inorganic pyrophosphatase
-
H+-PPase
-
-
-
-
inorganic diphosphatase
-
-
-
-
inorganic pyrophosphatase
-
-
-
-
PPase
-
-
-
-
pyrophosphatase, inorganic
-
-
-
-
Pyrophosphate phospho-hydrolase
-
-
-
-
Pyrophosphate phosphohydrolase
-
-
-
-
Pyrophosphate-energized inorganic pyrophosphatase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
diphosphate phosphohydrolase
Specificity varies with the source and with the activating metal ion. The enzyme from some sources may be identical with EC 3.1.3.1 (alkaline phosphatase) or EC 3.1.3.9 (glucose-6-phosphatase). cf. EC 7.1.3.1, H+-exporting diphosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-82-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O
?
show the reaction diagram
-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
?
CDP + H2O
?
show the reaction diagram
-
-
-
?
CTP + H2O
?
show the reaction diagram
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
most specific substrate
-
-
?
GDP + H2O
?
show the reaction diagram
-
-
-
?
GTP + H2O
?
show the reaction diagram
-
-
-
?
IDP + H2O
?
show the reaction diagram
-
-
-
?
ITP + H2O
?
show the reaction diagram
-
-
-
?
triphosphate + H2O
?
show the reaction diagram
-
-
-
?
UDP + H2O
?
show the reaction diagram
-
-
-
?
UDP-glucose + H2O
?
show the reaction diagram
-
-
-
?
UTP + H2O
?
show the reaction diagram
weakest substrate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphate + H2O
2 phosphate
show the reaction diagram
most specific substrate
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe3+
activates to a lesser extent than Mg2+
Mg2+
best activating ion
Mn2+
activates to a lesser extent than Mg2+
Ni2+
activates to a lesser extent than Mg2+
additional information
not activated by Zn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.43
ADP
at pH 9.5 and 95°C
4.13
ATP
at pH 9.5 and 95°C
3.12
CDP
at pH 9.5 and 95°C
3.61
CTP
at pH 9.5 and 95°C
0.173
diphosphate
at pH 9.5 and 95°C
3.56
GDP
at pH 9.5 and 95°C
3.63
GTP
at pH 9.5 and 95°C
3.47
IDP
at pH 9.5 and 95°C
5.01
ITP
at pH 9.5 and 95°C
1.37
Triphosphate
at pH 9.5 and 95°C
3.32
UDP
at pH 9.5 and 95°C
6.09
UDP-glucose
at pH 9.5 and 95°C
11.8
UTP
at pH 9.5 and 95°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19.7
ADP
at pH 9.5 and 95°C
91.7
ATP
at pH 9.5 and 95°C
51.3
CDP
at pH 9.5 and 95°C
94.2
CTP
at pH 9.5 and 95°C
1230
diphosphate
at pH 9.5 and 95°C
39.9
GDP
at pH 9.5 and 95°C
91.7
GTP
at pH 9.5 and 95°C
57.3
IDP
at pH 9.5 and 95°C
116
ITP
at pH 9.5 and 95°C
226
Triphosphate
at pH 9.5 and 95°C
27.5
UDP
at pH 9.5 and 95°C
29.7
UDP-glucose
at pH 9.5 and 95°C
99.1
UTP
at pH 9.5 and 95°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.44
ADP
at pH 9.5 and 95°C
22.2
ATP
at pH 9.5 and 95°C
16.4
CDP
at pH 9.5 and 95°C
26.1
CTP
at pH 9.5 and 95°C
7100
diphosphate
at pH 9.5 and 95°C
11.2
GDP
at pH 9.5 and 95°C
25.2
GTP
at pH 9.5 and 95°C
16.5
IDP
at pH 9.5 and 95°C
23.2
ITP
at pH 9.5 and 95°C
164
Triphosphate
at pH 9.5 and 95°C
8.28
UDP
at pH 9.5 and 95°C
4.87
UDP-glucose
at pH 9.5 and 95°C
8.4
UTP
at pH 9.5 and 95°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0559
with diphosphate as substrate, at pH 9.5 and 95°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 11
over 50% of the maximal activity is retained in moderately alkaline conditions (pH ranging from 7.5 to 11.0 at 95°C), while less than 15% of the maximal activity of the enzyme is retained at pH values over 12.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 100
the enzyme activity increases sharply from 80°C to 95°C. A moderate decrease in activity is observed between 95 and 100°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21740
2 * 21740, calculated from amino acid sequence
22000
2 * 22000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85 - 100
the enzyme shows half-lives of about 6850, 2760 and 850 min at 85, 95 and 100°C in the presence of Mg2+, respectively, as well as 3940, 1900, and 490 min at 85, 95 and 100°C in the absence of Mg2+, respectively
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yan, X.; Dong, Q.; Zheng, M.; Yang, Z.
Characterization of a family I-liked alkaline-resistant inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus furiosus for rapid determination of sugar nucleotidyltransferase at high temperature
J. Mol. Catal. B
98
15-20
2013
Pyrococcus furiosus (Q8U438), Pyrococcus furiosus ATCC 43587 (Q8U438)
-
Manually annotated by BRENDA team