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IUBMB Comments The enzyme from the leaves of Alangium lamarckii differs in enantiomeric specificity from EC 3.5.99.15 , deacetylisoipecoside synthase. The product is rapidly converted to demethylalangiside.
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deacetylipecoside + H2O = dopamine + secologanin
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dopamine-secologanine condensation
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deacetylipecoside dopamine-hydroyase (secologanin-forming)
The enzyme from the leaves of Alangium lamarckii differs in enantiomeric specificity from EC 3.5.99.15, deacetylisoipecoside synthase. The product is rapidly converted to demethylalangiside.
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dopamine + secologanin
(1R)-deacetylipecoside + H2O
dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
-
Substrates: -
?
dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
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Substrates: highly specific for dopamine
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dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
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Substrates: involved in production of tetrahydroquinoline monoterpene alkaloide derivates
?
dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
-
Substrates: -
?
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dopamine + secologanin
(1R)-deacetylipecoside + H2O
dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
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Substrates: involved in production of tetrahydroquinoline monoterpene alkaloide derivates
?
dopamine + secologanin
(1R)-deacetylipecoside + H2O
Alangium lamarckii
-
Substrates: -
?
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alangimarckine
Alangium lamarckii
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Ki: 0.01 mM
dehydroalangimarckine
Alangium lamarckii
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Ki: 0.01 mM
additional information
Alangium lamarckii
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not inhibited by dopamine, tyramine, tryptamine, cephaeline, emetine and tubulosine
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0.7
dopamine
Alangium lamarckii
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with fixed concentration of secologanin of 5 mM
0.9
secologanin
Alangium lamarckii
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with fixed concentration of dopamine of 5 mM
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0.078
Alangium lamarckii
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crude extract
43.02
Alangium lamarckii
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purified enzyme
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7.5
Alangium lamarckii
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-
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7.5
Alangium lamarckii
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-
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45
Alangium lamarckii
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-
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Alangium lamarckii
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-
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brenda
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Alangium lamarckii
-
-
brenda
Highest Expressing Human Cell Lines
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30000
Alangium lamarckii
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gel filtration, SDS-PAGE
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monomer
Alangium lamarckii
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gel filtration, SDS-PAGE
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-20 - 4°C, 50% loss of activity within 5 days, complete loss of activity within 7 days
Alangium lamarckii
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partial
Alangium lamarckii
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DeEknamkul, W.; Ounaroon, A.; Tanahashi, T.; Kutchan, T.; Zenk, M.H.
Enzymatic condensation of dopamine and secologanin by cell-free extracts of Alangium lamarckii
Phytochemistry
45
477-484
1997
Alangium lamarckii
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brenda
DeEknamkul, W.; Suttipanta, N.; Kutchan, T.
Purification and characterization of deacetylipecoside synthase from Alangium lamarckii Thw.
Phytochemistry
55
177-181
2000
Alangium lamarckii
brenda
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