Reference on EC 3.5.5.7 - Aliphatic nitrilase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kobayashi, M.; Yanak, N.; Nagasawa, T.; Yamada, H.
Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodocrous K22 and expression of its gene and identification of its active site residue
Biochemistry
31
9000-9007
1992
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Kobayashi, M.; Yanaka, N.; Nagasawa, T.; Yamada, H.
Purification and characterization of a novel nitrilase of Rhodococcus rhodochrous K22 that acts on aliphatic nitriles
J. Bacteriol.
172
4807-4815
1990
Rhodococcus rhodochrous, Rhodococcus rhodochrous K22
Levy-Schil, S.; Soubrier, F.; Crutz-Le Coq, A.M.; Faucher, D.; Crouzet, J.; Petre, D.
Aliphatic nitrilase from a soil-isolated Comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure
Gene
161
15-20
1995
Comamonas testosteroni
Dhillon, J.; Chhatre, S.; Shanker, R.; Shivaraman, N.
Transformation of aliphatic and aromatic nitriles by a nitrilase from Pseudomonas sp.
Can. J. Microbiol.
45
811-815
1999
Pseudomonas sp., Pseudomonas sp. S1
-
Chauhan, S.; Wu, S.; Blumerman, S.; Fallon, R.D.; Gavagan, J.E.; DiCosimo, R.; Payne, M.S.
Purification, cloning, sequencing and over-expression in Escherichia coli of a regioselective aliphatic nitrilase from Acidovorax facilis 72W
Appl. Microbiol. Biotechnol.
61
118-122
2003
Acidovorax facilis, Acidovorax facilis 72W
Dias, J.C.T.; Rezende, R.P.; Rosa, C.A.; Lachance, M.A.; Linardi, V.R.
Enzymatic degradation of nitriles by a Candida guilliermondii UFMG-Y65
Can. J. Microbiol.
46
525-531
2000
Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65
Brenner, C.
Catalysis in the nitrilase superfamily
Curr. Opin. Struct. Biol.
12
775-782
2002
Arabidopsis thaliana (P46011), Rhodococcus rhodochrous (Q03217)
Bergeron, S.; Chaplin, D.A.; Edwards, J.H.; Ellis, B.S.; Hill, C.L.; Holt-Tiffin, K.; Knight, J.R.; Mahoney, T.; Osborne, A.P.; Ruecroft, G.
Nitrilase-catalyzed desymmetrization of 3-hydroxyglutaronitrile: preparation of a statin side-chain intermediate
Org. Proc. Res. Dev.
10
661-665
2006
Pseudomonas fluorescens
-
Mueller, P.; Egorova, K.; Vorgias, C.E.; Boutou, E.; Trauthwein, H.; Verseck, S.; Antranikian, G.
Cloning, overexpression, and characterization of a thermoactive nitrilase from the hyperthermophilic archaeon Pyrococcus abyssi
Protein Expr. Purif.
47
672-681
2006
Pyrococcus abyssi, no activity in Aeropyrum pernix, no activity in Pyrococcus horikoshii, no activity in Pyrococcus furiosus, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
Hann, E.C.; Sigmund, A.E.; Fager, S.K.; Cooling, F.B.; Gavagan, J.E.; Bramucci, M.G.; Chauhan, S.; Payne, M.S.; DiCosimo, R.
Regioselective biocatalytic hydrolysis of (E,Z)-2-methyl-2-butenenitrile for production of (E)-2-methyl-2-butenoic acid
Tetrahedron
60
577-581
2004
Acidovorax facilis, Acidovorax facilis 72W
-
Yeom, S.J.; Kim, H.J.; Lee, J.K.; Kim, D.E.; Oh, D.K.
A determinant residue of substrate specificity in nitrilase from Rhodococcus rhodochrous ATCC 33278 for aliphatic and aromatic nitriles
Biochem. J.
415
401-407
2008
Rhodococcus rhodochrous, Rhodococcus rhodochrous (Q03217)
Holtze, M.S.; Sorensen, J.; Hansen, H.C.; Aamand, J.
Transformation of the herbicide 2,6-dichlorobenzonitrile to the persistent metabolite 2,6-dichlorobenzamide (BAM) by soil bacteria known to harbour nitrile hydratase or nitrilase
Biodegradation
17
503-510
2006
Pseudomonas putida, Pseudomonas fluorescens, Rhizobium sp., Pseudomonas putida 11388, Pseudomonas fluorescens 11387, Rhizobium sp. 11401
Mukherjee, C.; Zhu, D.; Biehl, E.R.; Hua, L.
Exploring the synthetic applicability of a cyanobacterium nitrilase as catalyst for nitrile hydrolysis
Eur. J. Org. Chem.
23
5238-5242
2006
Synechocystis sp.
-
Khandelwal, A.K.; Nigam, V.K.; Choudhury, B.; Mohan, M.K.; Ghosh, P.
Optimization of nitrilase production from a new thermophilic isolate
J. Chem. Technol. Biotechnol.
82
646-651
2007
Streptomyces sp., Streptomyces sp. MTCC 7546
-
Luo, H.; Fan, L.; Chang, Y.; Ma, J.; Yu, H.; Shen, Z.
Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli
Appl. Biochem. Biotechnol.
160
393-400
2008
Rhodococcus rhodochrous, Rhodococcus rhodochrous tg1-A6
Kim, J.S.; Tiwari, M.K.; Moon, H.J.; Jeya, M.; Ramu, T.; Oh, D.K.; Kim, I.W.; Lee, J.K.
Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5
Appl. Microbiol. Biotechnol.
83
273-283
2009
Pseudomonas fluorescens (Q4KCL8), Pseudomonas fluorescens, Pseudomonas fluorescens Pf-5 (Q4KCL8)
Nigam, V.K.; Khandelwal, A.K.; Gothwal, R.K.; Mohan, M.K.; Choudhury, B.; Vidyarthi, A.S.; Ghosh, P.
Nitrilase-catalysed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp.
J. Biosci.
34
21-26
2009
Streptomyces sp.
Bayer, S.; Birkemeyer, C.; Ballschmiter, M.
A nitrilase from a metagenomic library acts regioselectively on aliphatic dinitriles
Appl. Microbiol. Biotechnol.
89
91-98
2011
uncultured bacterium (E1A0Z9)
Thuku, R.; Brady, D.; Benedik, M.; Sewell, B.
Microbial nitrilases: Versatile, spiral forming, industrial enzymes
J. Appl. Microbiol.
106
703-727
2009
Acinetobacter sp., Acidovorax facilis, Pseudomonas sp., Pyrococcus abyssi, Rhodococcus rhodochrous (Q02068), Rhodococcus rhodochrous (Q03217), Synechocystis sp. (Q55949), Comamonas testosteroni (Q59329), Pseudomonas sp. S1, Acinetobacter sp. AK226, Rhodococcus rhodochrous K22 (Q02068), Acidovorax facilis 72W, Rhodococcus rhodochrous J1 (Q03217)
Sharma, N.; Kushwaha, R.; Sodhi, J.; Bhalla, T.
In silico analysis of amino acid sequences in relation to specificity and physiochemical properties of some microbial nitrilases
J. Proteomics Bioinform.
2
185-192
2009
Bradyrhizobium sp., Bradyrhizobium sp. ORS278, Methylibium petroleiphilum (A2SEG6), Pseudomonas syringae pv. syringae (Q500U1), Rhodococcus rhodochrous, Rhodococcus rhodochrous J1, Rhodococcus rhodochrous K22, Synechococcus elongatus (A0A0H3K0Y4)
-
He, Y.C.; Ma, C.L.; Xu, J.H.; Zhou, L.
A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry
Appl. Microbiol. Biotechnol.
89
817-823
2011
Alcaligenes sp., Rhodococcus erythropolis, Rhodococcus erythropolis CGMCC 1.2362, Alcaligenes sp. ECU0401
Fang, S.; An, X.; Liu, H.; Cheng, Y.; Hou, N.; Feng, L.; Huang, X.; Li, C.
Enzymatic degradation of aliphatic nitriles by Rhodococcus rhodochrous BX2, a versatile nitrile-degrading bacterium
Biores. Technol.
185
28-34
2015
Rhodococcus rhodochrous, Rhodococcus rhodochrous BX2
Yusuf, F.; Rather, I.A.; Jamwal, U.; Gandhi, S.G.; Chaubey, A.
Cloning and functional characterization of nitrilase from Fusarium proliferatum AUF-2 for detoxification of nitriles
Funct. Integr. Genomics
15
413-424
2015
Fusarium proliferatum (A0A0E3D8K7), Fusarium proliferatum AUF-2 (A0A0E3D8K7)
Zhang, L.; Yin, B.; Wang, C.; Jiang, S.; Wang, H.; Yuan, Y.A.; Wei, D.
Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Synechocystis sp. PCC6803
J. Struct. Biol.
188
93-101
2014
Synechocystis sp. (Q55949)
Wang, H.; Li, G.; Li, M.; Wei, D.; Wang, X.
A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization
World J. Microbiol. Biotechnol.
30
245-252
2014
Cereibacter sphaeroides (G5DDB2), Cereibacter sphaeroides LHS-305 (G5DDB2)
Sharma, N.; Verma, R.; Savitri, R.; Bhalla, T.C.
Classifying nitrilases as aliphatic and aromatic using machine learning technique
3 Biotech
8
68
2018
Agrobacterium rhizogenes, Variovorax paradoxus, Achromobacter xylosoxidans, Burkholderia gladioli, Comamonas testosteroni, Mesorhizobium loti, Afipia carboxidovorans, Rhizobium leguminosarum, Rhizoctonia solani, Rhodococcus rhodochrous, Starkeya novella, Sorangium cellulosum, Teredinibacter turnerae, Bradyrhizobium elkanii, Methyloversatilis universalis, Methylibium petroleiphilum, Janthinobacterium sp. Marseille, Paraburkholderia kururiensis, Saccharomonospora viridis, Burkholderia sp. BT03, Nocardia sp. C-14-1, Rhizobium leguminosarum bv. viciae 3841, Danaus plexippus, Polycyclovorans algicola, Methylobacterium sp. L2-4, Bosea sp. 117, Bradyrhizobium sp. th.b2, Azospirillum halopraeferens, Rhizobium sp. JGI 0001019-L19, Paraburkholderia mimosarum, Amycolatopsis taiwanensis, Variovorax sp. P21, Acidovorax oryzae, Methylobacterium sp. 88A, Methylopila sp. 73B, Xanthobacter sp. 126, Colletotrichum fioriniae, Marinomonas ushuaiensis, Betaproteobacteria bacterium, Cupriavidus sp. WS, Methylopila sp. M107, Serratia sp. M24T3, Bradyrhizobium sp. ORS 278 (A4YWK0), Shimwellia blattae (I2BBF1), Sphingopyxis alaskensis (Q1GTC0), Synechococcus elongatus PCC 6301 (Q31PZ9), Pseudomonas syringae pv. syringae (Q500U1), Colletotrichum fioriniae PJ7, Saccharomonospora viridis DSM 43017, Sorangium cellulosum So0157-2, Agrobacterium rhizogenes ATCC 15834, Variovorax paradoxus EPS, Marinomonas ushuaiensis DSM 15871, Sphingopyxis alaskensis DSM 13593 (Q1GTC0), Rhodococcus rhodochrous K22, Rhizoctonia solani 123E, Danaus plexippus F2, Pseudomonas syringae pv. syringae B728a (Q500U1), Shimwellia blattae ATCC 29907 (I2BBF1), Betaproteobacteria bacterium MOLA814, Afipia carboxidovorans OM5, Rhodococcus rhodochrous J1
Novikov, A.; Riabchenko, L.; Leonova, T.; Larikova, G.; Lavrov, K.; Glinskii, S.; Yanenko, A.
Bacterial strain Alcaligenes denitrificans C-32 containing two nitrilases with different substrate specificities
Appl. Biochem. Microbiol.
53
786-791
2017
Achromobacter denitrificans (A0A286S9Z8), Achromobacter denitrificans (A0A291PNG0), Achromobacter denitrificans C-32 (A0A286S9Z8), Achromobacter denitrificans C-32 (A0A291PNG0)
-
Fan, H.; Chen, L.; Sun, H.; Wang, H.; Ren, Y.; Wei, D.
A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production
Bioprocess Biosyst. Eng.
40
1271-1281
2017
Cupriavidus necator
Liu, D.; Xi, L.; Han, D.; Dou, K.; Su, S.; Liu, J.
Cloning, expression, and characterization of a novel nitrilase, PaCNit, from Pannonibacter carbonis Q4.6
Biotechnol. Lett.
41
583-589
2019
Pannonibacter carbonis, Pannonibacter carbonis Q4.6
Chen, Z.; Chen, H.; Ni, Z.; Tian, R.; Zhang, T.; Jia, J.; Yang, S.
Expression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8
J. Microbiol. Biotechnol.
25
1660-1669
2015
Thermotoga maritima (Q9WYX6)
Han, C.; Yao, P.; Yuan, J.; Duan, Y.; Feng, J.; Wang, M.; Wu, Q.; Zhu, D.
Nitrilase-catalyzed hydrolysis of 3-aminopropionitrile at high concentration with a tandem reaction strategy for shifting the reaction to beta-alanine formation
J. Mol. Catal. B
115
113-118
2015
Bradyrhizobium japonicum, Bradyrhizobium japonicum USDA110
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