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3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
3-cyano-L-alanine + H2O
L-aspartate + NH3
beta-cyano-L-alanine + H2O
L-aspartate + NH3
phenyl-3-propionitrile + H2O
phenyl-3-propionic acid + NH3
Substrates: -
Products: -
?
additional information
?
-
3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
Substrates: -
Products: -
?
3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
Substrates: -
Products: -
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
Substrates: -
Products: enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
Substrates: -
Products: enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: high activity substrate for isozyme complexes NIT4A/B2 and NIT4A/B1
Products: -
ir
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: -
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
Substrates: L-asparagine is formed as an intermediate
Products: -
?
additional information
?
-
-
Substrates: indole-3-acetonitrile is no substrate for NIT-T4
Products: -
?
additional information
?
-
-
Substrates: isoform NIT4B does not show beta-cyano-L-alanine-hydrolyzing activity
Products: -
?
additional information
?
-
Substrates: no substrate: indole-3-acetonitrile
Products: -
?
additional information
?
-
Substrates: no substrate: indole-3-acetonitrile
Products: -
?
additional information
?
-
-
Substrates: no substrates are various nitrile or amide compounds, overview
Products: -
?
additional information
?
-
-
Substrates: no substrates are various nitrile or amide compounds, overview
Products: -
?
additional information
?
-
-
Substrates: recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile
Products: -
?
additional information
?
-
-
Substrates: isoform NIT4B1 does not show any beta-cyano-L-alanine-hydrolyzing activity
Products: -
?
additional information
?
-
-
Substrates: does not use indole-3-acetonitrile as a substrate
Products: -
?
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Yanase, H.; Sakai, T.; Tonomura, K.
Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
Pseudomonas sp., Pseudomonas sp. 13
-
brenda
Piotrowski, M.; Volmer, J.J.
Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog
Plant Mol. Biol.
61
111-122
2006
Lupinus angustifolius (Q3LRV4), Lupinus angustifolius (Q5QGZ8)
brenda
Kriechbaumer, V.; Park, W.J.; Piotrowski, M.; Meeley, R.B.; Gierl, A.; Glawischnig, E.
Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis
J. Exp. Bot.
58
4225-4233
2007
Zea mays
brenda
Ishikawa, T.; Okazaki, K.; Kuroda, H.; Itoh, K.; Mitsui, T.; Hori, H.
Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development
Mol. Plant Pathol.
8
623-637
2007
Brassica rapa
brenda
Jenrich, R.; Trompetter, I.; Bak, S.; Olsen, C.E.; Moller, B.L.; Piotrowski, M.
Evolution of heteromeric nitrilase complexes in Poaceae with new functions in nitrile metabolism
Proc. Natl. Acad. Sci. USA
104
18848-18853
2007
Sorghum bicolor
brenda
Howden, A.J.; Harrison, C.J.; Preston, G.M.
A conserved mechanism for nitrile metabolism in bacteria and plants
Plant J.
57
243-253
2009
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
brenda
Gupta, N.; Balomajumder, C.; Agarwal, V.
Enzymatic mechanism and biochemistry for cyanide degradation: A review
J. Hazard. Mater.
176
1-13
2010
Chromobacterium violaceum, Escherichia coli, Priestia megaterium
brenda
Janowitz, T.; Trompetter, I.; Piotrowski, M.
Evolution of nitrilases in glucosinolate-containing plants
Phytochemistry
70
1680-1686
2009
Allium cepa, Arabidopsis thaliana, Brassica rapa, Capsella rubella, Carica papaya, Curcuma longa, Glycine max, Hordeum vulgare, Lactuca sativa, Linum usitatissimum, Lotus japonicus, Lupinus angustifolius, Manihot esculenta, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pinus taeda, Populus trichocarpa, Ricinus communis, Saccharum officinarum, Selaginella moellendorffii, Solanum lycopersicum, Sorghum bicolor, Tarenaya spinosa, Triticum aestivum, Tropaeolum majus, Vitis vinifera, Zea mays
brenda
O'Leary, B.; Preston, G.M.; Sweetlove, L.J.
Increased beta-cyanoalanine nitrilase activity improves cyanide tolerance and assimilation in Arabidopsis
Mol. Plant
7
231-243
2014
Pseudomonas fluorescens
brenda
Machingura, M.; Sidibe, A.; Wood, A.J.; Ebbs, S.D.
The beta-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana
Plant Physiol. Biochem.
63
159-169
2013
Arabidopsis thaliana
brenda
Acera, F.; Carmona, M.I.; Castillo, F.; Quesada, A.; Blasco, R.
A cyanide-induced 3-cyanoalanine nitrilase in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes strain CECT 5344
Appl. Environ. Microbiol.
83
e00089-17
2017
Pseudomonas oleovorans (W6R265), Pseudomonas oleovorans CECT 5344 (W6R265)
brenda