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3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
3-cyano-L-alanine + H2O
L-aspartate + asparagine
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
beta-cyano-L-alanine + H2O
L-aspartate + NH3
phenyl-3-propionitrile + H2O
phenyl-3-propionic acid + NH3
-
-
-
?
additional information
?
-
3-cyano-L-alanine + 2 H2O

L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + H2O

L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
high activity substrate for isozyme complexes NIT4A/B2 and NIT4A/B1
-
-
ir
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O

L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
additional information

?
-
-
indole-3-acetonitrile is no substrate for NIT-T4
-
-
?
additional information
?
-
-
isoform NIT4B does not show beta-cyano-L-alanine-hydrolyzing activity
-
-
?
additional information
?
-
no substrate: indole-3-acetonitrile
-
-
?
additional information
?
-
no substrate: indole-3-acetonitrile
-
-
?
additional information
?
-
-
no substrates are various nitrile or amide compounds, overview
-
-
?
additional information
?
-
-
no substrates are various nitrile or amide compounds, overview
-
-
?
additional information
?
-
-
recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile
-
-
?
additional information
?
-
-
isoform NIT4B1 does not show any beta-cyano-L-alanine-hydrolyzing activity
-
-
?
additional information
?
-
-
does not use indole-3-acetonitrile as a substrate
-
-
?
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NRL4B_LUPAN
350
0
38055
Swiss-Prot
other Location (Reliability: 2)
NRL4B_TOBAC
348
0
37534
Swiss-Prot
other Location (Reliability: 3)
NRL4_ARATH
355
0
38895
Swiss-Prot
other Location (Reliability: 3)
NRL4_ORYSJ
362
0
38676
Swiss-Prot
other Location (Reliability: 3)
NRL4A_LUPAN
349
0
37762
Swiss-Prot
other Location (Reliability: 3)
NRL4A_TOBAC
349
0
37555
Swiss-Prot
other Location (Reliability: 3)
A0A158S7C5_9SPHN
311
0
33363
TrEMBL
-
W6VD67_PSESZ
Pseudomonas sp. (strain GM41(2012))
307
0
32918
TrEMBL
-
C6CJF0_DICC1
Dickeya chrysanthemi (strain Ech1591)
308
0
33017
TrEMBL
-
A0A396I498_MEDTR
205
0
22701
TrEMBL
other Location (Reliability: 2)
A0A6I8LXC3_9PSEU
280
0
29675
TrEMBL
-
A0A212IW40_9DELT
315
0
34524
TrEMBL
-
K0KKW9_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
304
0
33351
TrEMBL
other Location (Reliability: 2)
E6W9U7_PANSA
Pantoea sp. (strain At-9b)
306
0
33273
TrEMBL
-
A0A2I0A9E6_9ASPA
351
0
37737
TrEMBL
other Location (Reliability: 4)
A0A212KM05_9PROT
311
0
33250
TrEMBL
-
A0A3B0RGR6_9ZZZZ
311
0
33930
TrEMBL
Secretory Pathway (Reliability: 5)
D4YM24_9MICO
283
0
31075
TrEMBL
-
F0Q9A2_ACIAP
Acidovorax avenae (strain ATCC 19860 / DSM 7227 / JCM 20985 / NCPPB 1011)
316
0
33638
TrEMBL
-
A0A251SW76_HELAN
344
0
37072
TrEMBL
other Location (Reliability: 3)
A0A6J4JBT1_9ACTN
325
0
34960
TrEMBL
-
W6VWG3_9PSED
307
0
32843
TrEMBL
-
A0A067Z3P3_GLUOY
321
0
35085
TrEMBL
-
A0A0B6AAE0_BACMB
Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512)
318
0
35116
TrEMBL
-
A0A1L8CLB8_9PROT
311
0
34480
TrEMBL
-
A0A2G9HT68_9LAMI
233
0
25494
TrEMBL
other Location (Reliability: 4)
H1KNZ3_METEX
318
0
33627
TrEMBL
-
A0A251VDZ8_HELAN
344
0
37140
TrEMBL
other Location (Reliability: 2)
A0A1W6KTS3_9RHIZ
325
0
35351
TrEMBL
-
T2LBZ8_9GAMM
311
0
33827
TrEMBL
-
A0A3B0R8Y0_9ZZZZ
161
0
18232
TrEMBL
other Location (Reliability: 2)
A0A6J4UTC9_9CHLR
312
0
33376
TrEMBL
-
A0A396IC97_MEDTR
125
0
13487
TrEMBL
other Location (Reliability: 5)
A0A072UKA3_MEDTR
350
0
37868
TrEMBL
other Location (Reliability: 2)
A0A069AD49_CLODI
308
0
34356
TrEMBL
-
W6R265_PSEP5
Pseudomonas pseudoalcaligenes (strain CECT 5344)
324
0
35380
TrEMBL
-
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Yanase, H.; Sakai, T.; Tonomura, K.
Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13
Agric. Biol. Chem.
47
473-482
1983
Pseudomonas sp., Pseudomonas sp. 13
-
brenda
Piotrowski, M.; Volmer, J.J.
Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog
Plant Mol. Biol.
61
111-122
2006
Lupinus angustifolius (Q3LRV4), Lupinus angustifolius (Q5QGZ8)
brenda
Kriechbaumer, V.; Park, W.J.; Piotrowski, M.; Meeley, R.B.; Gierl, A.; Glawischnig, E.
Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis
J. Exp. Bot.
58
4225-4233
2007
Zea mays
brenda
Ishikawa, T.; Okazaki, K.; Kuroda, H.; Itoh, K.; Mitsui, T.; Hori, H.
Molecular cloning of Brassica rapa nitrilases and their expression during clubroot development
Mol. Plant Pathol.
8
623-637
2007
Brassica rapa
brenda
Jenrich, R.; Trompetter, I.; Bak, S.; Olsen, C.E.; Moller, B.L.; Piotrowski, M.
Evolution of heteromeric nitrilase complexes in Poaceae with new functions in nitrile metabolism
Proc. Natl. Acad. Sci. USA
104
18848-18853
2007
Sorghum bicolor
brenda
Howden, A.J.; Harrison, C.J.; Preston, G.M.
A conserved mechanism for nitrile metabolism in bacteria and plants
Plant J.
57
243-253
2009
Pseudomonas fluorescens, Pseudomonas fluorescens SBW25
brenda
Gupta, N.; Balomajumder, C.; Agarwal, V.
Enzymatic mechanism and biochemistry for cyanide degradation: A review
J. Hazard. Mater.
176
1-13
2010
Priestia megaterium, Chromobacterium violaceum, Escherichia coli
brenda
Janowitz, T.; Trompetter, I.; Piotrowski, M.
Evolution of nitrilases in glucosinolate-containing plants
Phytochemistry
70
1680-1686
2009
Allium cepa, Arabidopsis thaliana, Brassica rapa, Carica papaya, Ricinus communis, Glycine max, Hordeum vulgare, Lactuca sativa, Linum usitatissimum, Lotus japonicus, Lupinus angustifolius, Solanum lycopersicum, Manihot esculenta, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pinus taeda, Populus trichocarpa, Saccharum officinarum, Sorghum bicolor, Triticum aestivum, Tropaeolum majus, Vitis vinifera, Zea mays, Curcuma longa, Selaginella moellendorffii, Capsella rubella, Tarenaya spinosa
brenda
O'Leary, B.; Preston, G.M.; Sweetlove, L.J.
Increased beta-cyanoalanine nitrilase activity improves cyanide tolerance and assimilation in Arabidopsis
Mol. Plant
7
231-243
2014
Pseudomonas fluorescens
brenda
Machingura, M.; Sidibe, A.; Wood, A.J.; Ebbs, S.D.
The beta-cyanoalanine pathway is involved in the response to water deficit in Arabidopsis thaliana
Plant Physiol. Biochem.
63
159-169
2013
Arabidopsis thaliana
brenda
Acera, F.; Carmona, M.I.; Castillo, F.; Quesada, A.; Blasco, R.
A cyanide-induced 3-cyanoalanine nitrilase in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes strain CECT 5344
Appl. Environ. Microbiol.
83
e00089-17
2017
Pseudomonas oleovorans (W6R265), Pseudomonas oleovorans CECT 5344 (W6R265)
brenda