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3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
3-cyano-L-alanine + H2O
L-aspartate + NH3
beta-cyano-L-alanine + H2O
L-aspartate + NH3
phenyl-3-propionitrile + H2O
phenyl-3-propionic acid + NH3
-
-
-
?
additional information
?
-
3-cyano-L-alanine + 2 H2O

L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + 2 H2O
L-aspartate + NH3
-
-
-
?
3-cyano-L-alanine + H2O

L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
L-asparagine is formed as an intermediate
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
high activity substrate for isozyme complexes NIT4A/B2 and NIT4A/B1
-
-
ir
3-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O

L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
-
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
-
L-asparagine is formed as an intermediate
-
-
?
additional information

?
-
-
indole-3-acetonitrile is no substrate for NIT-T4
-
-
?
additional information
?
-
-
isoform NIT4B does not show beta-cyano-L-alanine-hydrolyzing activity
-
-
?
additional information
?
-
no substrate: indole-3-acetonitrile
-
-
?
additional information
?
-
no substrate: indole-3-acetonitrile
-
-
?
additional information
?
-
-
no substrates are various nitrile or amide compounds, overview
-
-
?
additional information
?
-
-
no substrates are various nitrile or amide compounds, overview
-
-
?
additional information
?
-
-
recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile
-
-
?
additional information
?
-
-
isoform NIT4B1 does not show any beta-cyano-L-alanine-hydrolyzing activity
-
-
?
additional information
?
-
-
does not use indole-3-acetonitrile as a substrate
-
-
?
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