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Information on EC 3.5.5.1 - nitrilase and Organism(s) Saccharolobus solfataricus and UniProt Accession P95896

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase
IUBMB Comments
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
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This record set is specific for:
Saccharolobus solfataricus
UNIPROT: P95896
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Word Map
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
nitrilase, nitrilase 1, cyc705, nitrilase bll6402, nlase, nitrile aminohydrolase, benzonitrilase a, nitras-atii, nitmc-fb, nitrilase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SSO2122
ordered locus name
acetonitrilase
-
-
-
-
Arylacetonitrilase
-
-
-
-
benzonitrilase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nitrile aminohydrolase
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
157297-79-5
Arabidopsis thaliana columbiana and Lansberg gene NIT2
157575-01-4
Arabidopsis thaliana columbiana gene NIT3
157575-02-5
Arabidopsis thaliana columbiana gene NIT4
205331-43-7
Arabidopsis thaliana nit2 isoenzyme2
205394-78-1
Arabidopsis thaliana nit3 isoenzyme3
205394-80-5
Arabidopsis thaliana nit1 isoenzyme1
9024-90-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-anilinoproprionitrile + 2 H2O
3-anilinopropionate + NH3
show the reaction diagram
-
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
show the reaction diagram
-
-
-
?
cinnamonitrile + 2 H2O
cinnamic acid + NH3
show the reaction diagram
cinnamonitrile is a substrate for mutant enzyme K96R, no activity with wild-type enzyme
-
-
?
malononitrile + 2 H2O
malonate + NH3
show the reaction diagram
-
-
-
?
phenylglycinenitrile + 2 H2O
phenylglycine + NH3
show the reaction diagram
-
-
-
?
trichloroacetonitrile + 2 H2O
trichloroacetate + NH3
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phenylmethanesulfonyl fluoride
inhibits amide conversion more than 94% and completely suppresses nitrile hydrolysis
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000000103 - 0.000000157
3-anilinoproprionitrile
0.000005 - 0.000006
Benzonitrile
0.0000000097
Cinnamonitrile
pH 7.4, 70°C, mutant enzyme K96R
0.0000000495 - 0.000000609
Malononitrile
0.000000009 - 0.000000024
phenylglycinenitrile
0.0000000005 - 0.00000013
trichloroacetonitrile
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 0.28
3-anilinoproprionitrile
0.09
Benzonitrile
pH 7.4, 70°C, wild-type enzyme
0.016
Cinnamonitrile
pH 7.4, 70°C, mutant enzyme K96R
0.14 - 1.05
Malononitrile
0.016 - 0.04
phenylglycinenitrile
0.01 - 0.225
trichloroacetonitrile
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0006
pH 7.4, 70°C, substrate: benzonitrile
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K96R
retains the ability to hydrolyse amides but hydrolyses nitriles more efficiently than the wild-type enzyme
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
enzyme remains active for about 6 days
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cilia, E.; Fabbri, A.; Uriani, M.; Scialdone, G.G., Ammendola, S.
The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles. Ser195 and Cys145 are predicted to be the active site nucleophiles
FEBS J.
272
4716-4724
2005
Saccharolobus solfataricus (P95896), Saccharolobus solfataricus P2 (P95896)
Manually annotated by BRENDA team