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EC Tree
IUBMB Comments Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
The taxonomic range for the selected organisms is: Talaromyces marneffei The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
nitrilase, nitrilase 1, nitrilase bll6402,
cyc705 , nitrile aminohydrolase, benzonitrilase a, nlase, nitras-atii,
nitmc-fb , nitrilase i,
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Arylacetonitrilase
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-
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nitrile aminohydrolase
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
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157297-79-5
Arabidopsis thaliana columbiana and Lansberg gene NIT2
157575-01-4
Arabidopsis thaliana columbiana gene NIT3
157575-02-5
Arabidopsis thaliana columbiana gene NIT4
205331-43-7
Arabidopsis thaliana nit2 isoenzyme2
205394-78-1
Arabidopsis thaliana nit3 isoenzyme3
205394-80-5
Arabidopsis thaliana nit1 isoenzyme1
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2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
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-
-
?
3-chlorobenzonitrile + 2 H2O
3-chlorobenzoate + NH3
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-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
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-
-
?
4-chlorobenzonitrile + 2 H2O
4-chlorobenzoate + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
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-
-
?
benzonitrile + 2 H2O
benzoate + NH3
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-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
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-
-
?
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2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
3-chlorobenzonitrile + 2 H2O
3-chlorobenzoate + NH3
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
4-chlorobenzonitrile + 2 H2O
4-chlorobenzoate + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
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-
?
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11.7
2-Cyanopyridine
pH 8.0, 30°C, recombinant enzyme
11.1
3-chlorobenzonitrile
pH 8.0, 30°C, recombinant enzyme
34.4
3-Cyanopyridine
pH 8.0, 30°C, recombinant enzyme
2.6
4-chlorobenzonitrile
pH 8.0, 30°C, recombinant enzyme
15.4
4-cyanopyridine
pH 8.0, 30°C, recombinant enzyme
7.9
Benzonitrile
pH 8.0, 30°C, recombinant enzyme
9.1
phenylacetonitrile
pH 8.0, 30°C, recombinant enzyme
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0.24
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.7
recombinant enzyme coexpressed with GroEL/ES, pH 8.0, 30°C
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UniProt
brenda
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6.5 - 8.7
recombinant enzyme in cell-free extract, room temperature, 2 h, 70% activity remaining at pH 6.5 and pH 8.7, profile overview
718670
7
purified recombinant enzyme, room temperature, 2 h, completely stable
718670
9.5
and above, purified recombinant enzyme, room temperature, 2 h, no activity remaining
718670
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25 - 35
recombinant enzyme in cell-free extract, pH 8.0, 1 h, stable
40
recombinant enzyme in cell-free extract, pH 8.0, 1 h, 20% activity remaining
55
and above, recombinant enzyme in cell-free extract, pH 8.0, 1 h, no activity remaining
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recombinant enzyme 4fold from Escherichia coli strain BL21-Gold (DE3), co-purifiication with GroEL
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expression in Escherichia coli strain BL21-Gold (DE3), co-expression of GroEL/ES
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Petrickova, A.; Vesela, A.B.; Kaplan, O.; Kubac, D.; Uhnakova, B.; Malandra, A.; Felsberg, J.; Rinagelova, A.; Weyrauch, P.; K?en, V.; Bezouska, K.; Martinkova, L.
Purification and characterization of heterologously expressed nitrilases from filamentous fungi
Appl. Microbiol. Biotechnol.
93
1553-1561
2012
Fusarium verticillioides (Q19A54), Fusarium verticillioides, Talaromyces marneffei (B6Q5I3), Talaromyces marneffei ATCC 18224 (B6Q5I3), Talaromyces marneffei ATCC 18224
brenda