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Information on EC 3.5.4.6 - AMP deaminase and Organism(s) Arabidopsis thaliana and UniProt Accession O80452

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.6 AMP deaminase
IUBMB Comments
cf. EC 3.5.4.17 adenosine-phosphate deaminase.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O80452
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
+
=
+
Synonyms
amp deaminase, ampd1, amp-deaminase, adenylate deaminase, ampd2, myoadenylate deaminase, ampd3, adenosine monophosphate deaminase, muscle amp deaminase, ampda, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
embryonic factor 1
5-adenylate deaminase
-
-
-
-
5-adenylic acid deaminase
-
-
-
-
5-AMP aminohydrolase
-
-
-
-
5-AMP deaminase
-
-
-
-
adenosine 5-monophosphate deaminase
-
-
-
-
adenosine 5-phosphate aminohydrolase
-
-
-
-
adenosine 5-phosphate deaminase
-
-
-
-
adenosine monophosphate deaminase
-
-
-
-
adenyl deaminase
-
-
-
-
adenylate aminohydrolase
-
-
-
-
adenylate deaminase
-
-
-
-
adenylate desaminase
-
-
-
-
adenylic acid deaminase
-
-
-
-
adenylic deaminase
-
-
-
-
AMP aminase
-
-
-
-
AMP deaminase H-type
-
-
-
-
deaminase, adenylate
-
-
-
-
EMBRYONIC FACTOR1
-
-
Erythrocyte AMP deaminase
-
-
-
-
Heart-type AMPD
-
-
-
-
Myoadenylate deaminase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amidine hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
AMP aminohydrolase
cf. EC 3.5.4.17 adenosine-phosphate deaminase.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-10-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AMP + H2O
IMP + NH3
show the reaction diagram
the enzyme converts AMP to IMP to maintain the energy potential
-
-
?
AMP + H2O
IMP + NH3
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
AMP + H2O
IMP + NH3
show the reaction diagram
the enzyme converts AMP to IMP to maintain the energy potential
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
coformycin 5'-phosphate
-
1-bromo-4-[2-(8-hydroxy-7,8-dihydroimidazo[4,5-d][1,3]diazepin-3(6H)-yl)ethyl]-5,6,7,8-tetrahydronaphthalene-2-carboxylic acid
-
-
3-[2-(3-carboxy-5,6,7,8-tetrahydronaphthyl)-ethyl]imidazo[2,1-f][1,2,4]triazine
-
-
3-[2-(8-hydroxy-7,8-dihydroimidazo[4,5-d][1,3]diazepin-3(6H)-yl)ethyl]benzoic acid
-
-
3-[2-(imidazo[2,1-f][1,2,4]triazin-7-yl)ethyl]benzoic acid
-
-
4-[2-(8-hydroxy-7,8-dihydroimidazo[4,5-d][1,3]diazepin-3(6H)-yl)ethyl]-5,6,7,8-tetrahydronaphthalene-2-carboxylic acid
-
-
adenine
-
0.25 mM
coformycin 5'-phosphate
-
extremely potent AMPD inhibitor
deaminoformycin
-
0.0003 mM, strong inhibitor
deaminoformycin 5'-monophosphate
-
potent inhibitor
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000601
deaminoformycin 5'-monophosphate
-
in 25 mM imidazole, pH 6.5, 150 mM potassium chloride, 2.4 mM AMP, and 0.2 mg/ml bovine serum albumin
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
3-[2-(3-carboxy-5,6,7,8-tetrahydronaphthyl)-ethyl]imidazo[2,1-f][1,2,4]triazine
Arabidopsis thaliana
-
pH and temperature not specified in the publication
1.4
3-[2-(imidazo[2,1-f][1,2,4]triazin-7-yl)ethyl]benzoic acid
Arabidopsis thaliana
-
pH and temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
significantly increased FAC1 expression in the zygote, early embryo and endosperm. During somatic embryogenesis, a high level of FAC1 expression is observed in developing embryos including putative embryogenic cells. FAC1 represents one of the earliest expressed genes known in plants. It may act through AMP depletion to provide sufficient energy for the zygote to proceed through development
Manually annotated by BRENDA team
significantly increased FAC1 expression in the zygote, early embryo and endosperm
Manually annotated by BRENDA team
significantly increased FAC1 expression in the zygote, early embryo and endosperm
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
reversible association between the catalytic domain of the enzyme and the intracellular membrane
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AMPD_ARATH
839
1
95130
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3.3 A globular catalytic domain X-ray crystal structure with a bound herbicide-based transition state inhibitor (coformycin 5'-phosphate)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Sf9 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Han, B.W.; Bingman, C.A.; Mahnke, D.K.; Bannen, R.M.; Bednarek, S.Y.; Sabina, R.L.; Phillips, G.N.
Membrane association, mechanism of action, and structure of Arabidopsis embryonic factor 1 (FAC1)
J. Biol. Chem.
281
14939-14947
2006
Arabidopsis thaliana (O80452)
Manually annotated by BRENDA team
Xu, J.; Zhang, H.Y.; Xie, C.H.; Xue, H.W.; Dijkhuis, P.; Liu, C.M.
EMBRYONIC FACTOR 1 encodes an AMP deaminase and is essential for the zygote to embryo transition in Arabidopsis
Plant J.
42
743-756
2005
Arabidopsis thaliana (O80452)
Manually annotated by BRENDA team
Sabina, R.L.; Paul, A.L.; Ferl, R.J.; Laber, B.; Lindell, S.D.
Adenine nucleotide pool perturbation is a metabolic trigger for AMP deaminase inhibitor-based herbicide toxicity
Plant Physiol.
143
1752-1760
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Lindell, S.; Maechling, S.; Sabina, R.
Synthesis and biochemical testing of 3-(carboxyphenylethyl)imidazo[2,1-f] [1,2,4]triazines as inhibitors of AMP deaminase
ACS Med. Chem. Lett.
1
286-289
2010
Arabidopsis thaliana, Homo sapiens
Manually annotated by BRENDA team