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Information on EC 3.5.4.5 - cytidine deaminase and Organism(s) Mus musculus and UniProt Accession P56389

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     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase
IUBMB Comments
Contains zinc. Catalyses the deamination of cytidine and 2'-deoxycytidine with similar efficiencies. The enzyme, which is widely distributed among organisms, is involved in salvage of both exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
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This record set is specific for:
Mus musculus
UNIPROT: P56389
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
cytidine deaminase, activation-induced cytidine deaminase, aicda, deoxycytidine deaminase, cr deaminase, cyd deaminase, t-cda, cytosine nucleoside deaminase, cytidine aminohydrolase, yeast cytidine deaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
activation-induced cytidine deaminase
-
-
-
-
AICDA
-
-
-
-
Cytidine aminohydrolase
-
-
-
-
cytosine nucleoside deaminase
-
-
-
-
DCD
-
-
-
-
dCDA
-
-
-
-
mammalian deminase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
-
-
-
-
amidine hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
cytidine/2'-deoxycytidine aminohydrolase
Contains zinc. Catalyses the deamination of cytidine and 2'-deoxycytidine with similar efficiencies. The enzyme, which is widely distributed among organisms, is involved in salvage of both exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-56-6
-
9025-06-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxycytidine + H2O
2'-deoxyuridine + NH3
show the reaction diagram
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
1-beta-D-arabinofuranosylcytosine + H2O
?
show the reaction diagram
2',5'-anhydroarabinosylcytosine + H2O
2',5'-anhydroarabinosyluracil + NH3
show the reaction diagram
-
-
-
-
?
2'-deoxycytidine + H2O
2'-deoxyuridine + NH3
show the reaction diagram
-
-
-
-
?
5'-bromoarabinosylcytosine + H2O
5'-bromoarabinosyluracil + NH3
show the reaction diagram
-
-
-
-
?
5'-chloroarabinosylcytosine + H2O
5'-chloroarabinosyluracil + NH3
show the reaction diagram
-
-
-
-
?
5'-methyl-2'-deoxycytidine + H2O
5'-methyl-2-deoxyuridine + NH3
show the reaction diagram
-
-
-
-
?
5-bromodeoxycytidine + H2O
5-bromodeoxyuridine + NH3
show the reaction diagram
-
-
-
-
?
5-iodocytidine + H2O
5-iodouridine + NH3
show the reaction diagram
-
-
-
-
?
5-iododeoxycytidine + H2O
5-iododeoxyuridine + NH3
show the reaction diagram
-
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
-
-
-
-
?
cytosine arabinoside + H2O
uridine arabinoside + NH3
show the reaction diagram
-
-
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
show the reaction diagram
N-4-monoacetylarabinosylcytosine
uracil arabinoside + carbamate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxycytidine + H2O
2'-deoxyuridine + NH3
show the reaction diagram
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
-
-
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-deazauridine
-
tetrahydrouridine
-
1-(beta-D-ribofuranosyl)-2-pyrimidone
-
-
2-beta-D-ribofuranosyl-1,2,4-triazol-3-one
-
-
3,4,5,6-Tetrahydrouridine
6-thioguanine
-
-
DTNB
-
-
dUTP
-
-
Mersalyl acid
-
-
N-ethylmaleimide
-
-
p-mercuribenzoate
tetrahydrouridine
-
i.e. THU, acts as as a transition-state analogue of the natural substrate cytidine, quantitative determination of the cytidine deaminase inhibitor in mouse plasma by liquid chromatography/electrospray ionization tandem mass spectrometry, pharmacokinetics, overview
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.063
cytidine
-
normal and leukemic spleen enzyme
0.079 - 0.34
deoxycytidine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
an active-site Thr/Ser is conserved in mammalian DNA cytidine deaminases, overview
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDD_MOUSE
146
0
16131
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
homotetramer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with tetrahydrouridine, 3-deazauridine, or cytidine. Two alternate conformations of R68 influence zinc-product interaction
purified recombinant CDA complexed with either tetrahydrouridine, 3-deazauridine, or cytidine, hanging drop vapor diffusion technique, 25°C, protein solution, containing 10-15 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 1 mM DTT, and 5 mM ligand, is mixed with crystallization solution containing 2.0 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, crystals appear after a few hours or several days, respectively, X-ray diffraction structure determination and analysis at 1.48-2.36 A resolutions, molecular replacement
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity
-
-20°C, 0.005 M dithiothreitol, enzymatic activity is stable for many weeks
-
4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain BL21 by two steps of anion exchange chromatography, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme expression in Escherichia coli strain BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ipata, P.L.; Cercignani, G.
Cytosine and cytidine deaminase from yeast
Methods Enzymol.
51
394-401
1978
Saccharomyces cerevisiae, Escherichia coli, Mus musculus, Mus musculus BALB/c
Manually annotated by BRENDA team
Cullen, B.R.; Bick, M.D.
Bromodeoxyuridine induction of deoxycytidine deaminase activity in a hamster cell line
Biochim. Biophys. Acta
517
158-168
1978
Cricetinae, Mus musculus
Manually annotated by BRENDA team
Chan, T.S.; Lakhchaura, B.D.; Hsu, T.F.
Differences in deoxycytidine metabolism in mouse and rat
Biochem. J.
210
367-371
1983
Mus musculus
Manually annotated by BRENDA team
Kang, M.S.; Rhee, J.G.; Cho, J.M.
Metabolism of pyrimidine deoxyribonucleotides and heat-resistivity of CdR-aminohydrolase in the mouse small intestine
Korean J. Zool.
17
107-116
1974
Mus musculus
-
Manually annotated by BRENDA team
Wang, T.P.; Sable, H.Z.; Lampen, J.O.
Enzymatic deamination of cytosine nucleosides
J. Biol. Chem.
184
17-28
1950
Bos taurus, Saccharomyces cerevisiae, Oryctolagus cuniculus, Escherichia coli, Mus musculus, Escherichia coli 15 (ATCC 9723), Mus musculus BALB/c
Manually annotated by BRENDA team
Cohen, R.M.; Wolfenden, R.
Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine
J. Biol. Chem.
246
7561-7565
1971
Escherichia coli, Escherichia coli B / ATCC 11303, Homo sapiens, Mus musculus, Mus musculus BALB/c, Ovis aries, Saccharomyces cerevisiae
Manually annotated by BRENDA team
Hosono, H.; Kuno, S.
The purification and properties of cytidine deaminase from Escherichia coli
J. Biochem.
74
797-803
1973
Bacillus cereus, Saccharomyces cerevisiae, Escherichia coli, Ovis aries, Mus musculus, Mus musculus BALB/c, Escherichia coli Y-70-272
Manually annotated by BRENDA team
Wentworth, D.F.; Wolfenden, R.
Cytidine deaminases (from Escherichia coli and human liver)
Methods Enzymol.
51
401-407
1978
Canis lupus familiaris, Cavia porcellus, Columba sp., Escherichia coli, Escherichia coli B / ATCC 11303, Felis catus domesticus, Homo sapiens, Macaca mulatta, Mus musculus, Mus musculus BALB/c, Oryctolagus cuniculus, Rana sp., Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Rothman, I.K.; Malathi, V.G.; Silber, R.
Cytidine deaminase from leukemic mouse spleen
Methods Enzymol.
51
408-412
1978
Homo sapiens, Mus musculus, Mus musculus BALB/c
Manually annotated by BRENDA team
Kara, J.; Bartova, M.; Ryba, M.; Hrebabecky, H.; Brokes, J.; Novotny, L.; Baranek, J.
Sensitivity of some arabinosylcytosine derivatives to enzymatic deamination by cytidine deaminase from mouse kidney
Collect. Czech. Chem. Commun.
47
2824-2830
1982
Mus musculus, Mus musculus BALB/c
-
Manually annotated by BRENDA team
Ashley, G.W.; Bartlett, P.A.
Purification and properties of cytidine deaminase from escherichia coli
J. Biol. Chem.
259
13615-13620
1984
Escherichia coli, Escherichia coli B / ATCC 11303, Homo sapiens, Mus musculus, Mus musculus BALB/c, Ovis aries, Saccharomyces cerevisiae, Zea mays
Manually annotated by BRENDA team
Holy, A.; Ludzisa, A.; Votruba, I.; Sediva, K.
Preparation of analogues of cytosine and 2-pyrimidinone nucleosides and their effect on bacterial (Escherichia coli A19) cytidine aminohydrolase
Collect. Czech. Chem. Commun.
50
393-417
1985
Saccharomyces cerevisiae, Escherichia coli, Macaca mulatta, Mus musculus, Mus musculus BALB/c, Escherichia coli A19
-
Manually annotated by BRENDA team
Nygaard, P.
On the role of cytidine deaminase in cellular metabolism
Adv. Exp. Med. Biol.
195B
415-420
1986
Saccharomyces cerevisiae, Escherichia coli, Ovis aries, Homo sapiens, Mus musculus, no activity in Lactobacillus sp., no activity in Moraxella sp., no activity in Pseudomonas sp., Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays, Mus musculus BALB/c
Manually annotated by BRENDA team
Cacciamani, T.; Vita, A.; Cristalli, G.; Vincenzetti, S.; Natalini, P.; Ruggieri, S.; Amici, A.; Magni, G.
Purification of human cytidine deaminase: Molecular and enzymatic characterization and inhibition by synthetic pyrimidine analogs
Arch. Biochem. Biophys.
290
285-292
1991
Canis lupus familiaris, Gallus gallus, Escherichia coli, Homo sapiens, Mus musculus, no activity in Lactobacillus sp., no activity in Moraxella sp., no activity in Pseudomonas sp., Mus musculus BALB/c
Manually annotated by BRENDA team
Barchi, J.J.; Haces, A.; Marquez, V.E.; McCormack, J.J.
Inhibition of cytidine deaminase by derivatives of 1-(beta-D-ribofuranosyl)-dihydropyrimidin-2-one(zebularine)
Nucleosides Nucleotides
11
1781-1793
1992
Homo sapiens, Mus musculus, Mus musculus BALB/c
-
Manually annotated by BRENDA team
Vincenzetti, S.; Cambi, A.; Neuhard, J.; Schnorr, K.; Grelloni, M.; Vita, A.
Cloning, expression, and purification of cytidine deaminase from Arabidopsis thaliana
Protein Expr. Purif.
15
8-15
1999
Arabidopsis thaliana, Bacillus subtilis, Gallus gallus, Escherichia coli, Haemophilus influenzae, Ovis aries, Mus musculus, Escherichia coli SO5201, Mus musculus BALB/c
Manually annotated by BRENDA team
Teh, A.H.; Kimura, M.; Yamamoto, M.; Tanaka, N.; Yamaguchi, I.; Kumasaka, T.
The 1.48 A resolution crystal structure of the homotetrameric cytidine deaminase from mouse
Biochemistry
45
7825-7833
2006
Mus musculus (P56389), Mus musculus
Manually annotated by BRENDA team
Parise, R.A.; Egorin, M.J.; Eiseman, J.L.; Joseph, E.; Covey, J.M.; Beumer, J.H.
Quantitative determination of the cytidine deaminase inhibitor tetrahydrouridine (THU) in mouse plasma by liquid chromatography/electrospray ionization tandem mass spectrometry
Rapid Commun. Mass Spectrom.
21
1991-1997
2007
Mus musculus
Manually annotated by BRENDA team