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Information on EC 3.5.4.43 - hydroxydechloroatrazine ethylaminohydrolase
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3.5.4.43 hydroxydechloroatrazine ethylaminohydrolaseIUBMB Comments
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
adenosine aminohydrolase 3, hydroxyatrazine ethylaminohydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosine aminohydrolase 3
AtzB
EC 3.5.99.3
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formerly
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FRAAL1473
FraEuI1c_5875
HAEA
hydroxyatrazine ethylaminohydrolase
Hydroxyatrazine hydrolase
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additional information
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the enzyme belongs to the amidohydrolase superfamily
AtzB
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AtzB
Clavibacter michiganese ATZ1
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HAEA
Clavibacter michiganese ATZ1
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hydroxyatrazine ethylaminohydrolase
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hydroxyatrazine ethylaminohydrolase
Clavibacter michiganese ATZ1
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine
Involved in a pathway by which the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, is degraded in bacteria via N-isopropylammelide, 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of C-N bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
hydroxyatrazine ethylaminohydrolase
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
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CAS REGISTRY NUMBER
COMMENTARY
188496-88-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
?
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?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
?
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?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-chloro-4-amino-6-hydroxy-s-triazine + H2O
4-amino-2,6-dihydroxy-s-triazine + HCl
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ammelide is the sole reaction product
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?
2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
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?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
?
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
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ir
4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O
?
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?
4-amino-6-ethylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + ethylamine
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?
4-amino-6-isopropylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + isopropylamine
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?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
?
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
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i.e. hydroxyatrazine
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
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i.e. hydroxyatrazine
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
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i.e. hydroxyatrazine
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
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i.e. hydroxyatrazine
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?
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
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ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
Clavibacter michiganese ATZ1
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ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
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ir
additional information
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the isolated strain is capable of degrading atrazine. Displacement of the three substituents on the s-triazine ring is mediated by three enzymatic steps encoded by the genes atzA, atzB, and atzC. AtzB catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide
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additional information
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the enzyme performs deamination and dechlorination, substrate specificity, overview
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
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ir
additional information
?
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the isolated strain is capable of degrading atrazine. Displacement of the three substituents on the s-triazine ring is mediated by three enzymatic steps encoded by the genes atzA, atzB, and atzC. AtzB catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
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i.e. hydroxyatrazine
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?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
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i.e. hydroxyatrazine
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?
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
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ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
Clavibacter michiganese ATZ1
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ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
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ir
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from Manfredi soil in Argentina used to grow corn and are treated with atrazine for weed control during 3 years, gene atzB
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brenda
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Pseudoaminobacter sp.
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brenda
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Nocardiodes sp.
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brenda
use of atrazine as sole nitrogen source, but not as sole carbon source. Comparison of degradation products with those from Pseudoaminobacter sp. and Nocardiodes sp.
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brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
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the enzyme is involved in degradation of the herbicide atrazine
physiological function
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strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. In the growth medium, the amount of atrazine decreases rapidly, and hydroxyatrazine is observed to accumulate transiently and then undergo gradual degradation accompanied by the accumulation of cyanuric acid
physiological function
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strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. In the growth medium, the amount of atrazine decreases rapidly, and hydroxyatrazine is observed to accumulate transiently and then undergo gradual degradation accompanied by the accumulation of cyanuric acid
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A7C0W258_9BACT
193
0
22022
TrEMBL
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C7PZT0_CATAD
Catenulispora acidiphila (strain DSM 44928 / JCM 14897 / NBRC 102108 / NRRL B-24433 / ID139908)
455
0
47687
TrEMBL
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A0A424T2Q1_9PROT
131
0
14797
TrEMBL
-
V9VRM1_9RHOB
446
0
47675
TrEMBL
-
A0A0R2TGN2_9RHOB
446
0
47790
TrEMBL
-
Q472L9_CUPPJ
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197)
493
0
52989
TrEMBL
-
A0A098TJW9_9CYAN
472
0
51959
TrEMBL
-
A0A2P9I0E2_9ACTN
463
0
49479
TrEMBL
-
A0P3R3_ROSAI
Roseibium aggregatum (strain ATCC 25650 / DSM 13394 / JCM 20685 / NBRC 16684 / NCIMB 2208 / IAM 12614 / B1)
467
0
50358
TrEMBL
-
A0A2M7F3F2_9RHOB
185
0
20148
TrEMBL
-
B3R761_CUPTR
Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 / R1)
467
0
49740
TrEMBL
-
A0A072Y9X7_CLONO
461
0
51567
TrEMBL
-
A0A7V5LUK9_9BACT
185
0
20124
TrEMBL
-
A0A160TGE1_9ZZZZ
449
0
49386
TrEMBL
other Location (Reliability: 5)
A0A0B4XDW6_9HYPH
459
0
50176
TrEMBL
-
C5AWX2_METEA
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
452
0
48945
TrEMBL
-
Q0FT42_SALBH
Salipiger bermudensis (strain DSM 26914 / JCM 13377 / KCTC 12554 / HTCC2601)
446
0
47494
TrEMBL
-
A0A656G479_PSEA0
451
0
48853
TrEMBL
-