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Information on EC 3.5.4.33 - tRNA(adenine34) deaminase and Organism(s) Escherichia coli and UniProt Accession P68398

for references in articles please use BRENDA:EC3.5.4.33
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IUBMB Comments
The enzyme is involved in editing of tRNA. The active site contains Zn2+ .
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This record set is specific for:
Escherichia coli
UNIPROT: P68398
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Word Map
  • 3.5.4.33
  • deamination
  • wobble
  • anticodons
  • heterodimeric
  • a-to-i
  • deaminases
  • chloroplast
  • decode
  • intellectual
  • trna-specific
  • disability
  • trypanosoma
  • trnaarg
  • trypanosomatids
  • adats
  • base-pairing
  • cytidine-to-uridine
  • post-transcriptionally
  • brucei
The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
adenine34 in tRNA
+
=
hypoxanthine34 in tRNA
+
Synonyms
adat2/3, tada protein, trna:adenosine 34 deaminase, attada, sptad, adat2-adat3 complex, trna a:34 deaminase, trna adenosine deaminase arginine, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tadA/ecADAT2
-
tadA protein
-
-
tRNA:A34 deaminase
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA(adenine34) aminohydrolase
The enzyme is involved in editing of tRNA. The active site contains Zn2+ [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
-
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
disruption of the wild-type chromosomal copy of tadA results in viable Escherichia coli only when an additional plasmid-borne tadA gene is also present. TadA is essential for viability of eubacterial organisms
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18718
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence
homodimer
-
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D64E
D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichi coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luo, M.; Schramm, V.L.
Transition state structure of E. coli tRNA-specific adenosine deaminase
J. Am. Chem. Soc.
130
2649-2655
2008
Escherichia coli
Manually annotated by BRENDA team
Elias, Y.; Huang, R.H.
Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA
Biochemistry
44
12057-12065
2005
Agrobacterium tumefaciens (A9CK16), Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
Manually annotated by BRENDA team
Su, A.A.; Randau, L.
A-to-I and C-to-U editing within transfer RNAs
Biochemistry
76
932-937
2011
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Streptococcus pyogenes
Manually annotated by BRENDA team
Wolf, J.; Gerber, A.P.; Keller, W.
tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli
EMBO J.
21
3841-3851
2002
Escherichia coli (P68398), Escherichia coli
Manually annotated by BRENDA team