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Information on EC 3.5.4.33 - tRNA(adenine34) deaminase and Organism(s) Aquifex aeolicus and UniProt Accession O67050

for references in articles please use BRENDA:EC3.5.4.33
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EC Tree
IUBMB Comments
The enzyme is involved in editing of tRNA. The active site contains Zn2+ .
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This record set is specific for:
Aquifex aeolicus
UNIPROT: O67050
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Word Map
  • 3.5.4.33
  • deamination
  • wobble
  • anticodons
  • heterodimeric
  • a-to-i
  • deaminases
  • chloroplast
  • decode
  • intellectual
  • trna-specific
  • disability
  • trypanosoma
  • trnaarg
  • trypanosomatids
  • adats
  • base-pairing
  • cytidine-to-uridine
  • post-transcriptionally
  • brucei
The taxonomic range for the selected organisms is: Aquifex aeolicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
adenine34 in tRNA
+
=
hypoxanthine34 in tRNA
+
Synonyms
adat2/3, tada protein, trna:adenosine 34 deaminase, attada, sptad, adat2-adat3 complex, trna a:34 deaminase, trna adenosine deaminase arginine, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tadA protein
-
-
tRNA:A34 deaminase
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA(adenine34) aminohydrolase
The enzyme is involved in editing of tRNA. The active site contains Zn2+ [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
show the reaction diagram
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
show the reaction diagram
the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
TadA contains endogenous Zn2+ ions. Zn2+ is tetrahedrally coordinated by His52, Cys82, Cys85, and a water
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, 1.8 A resolution. The large cavity formed around the catalytic site with the zinc ion seems to be important for accommodating the anticodon loop stem of tRNAArg-2
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichi coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kuratani, M.; Ishii, R.; Bessho, Y.; Fukunaga, R.; Sengoku, T.; Shirouzu, M.; Sekine, S.; Yokoyama, S.
Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus
J. Biol. Chem.
280
16002-16008
2005
Aquifex aeolicus (O67050)
Manually annotated by BRENDA team
Elias, Y.; Huang, R.H.
Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA
Biochemistry
44
12057-12065
2005
Agrobacterium tumefaciens (A9CK16), Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
Manually annotated by BRENDA team
Su, A.A.; Randau, L.
A-to-I and C-to-U editing within transfer RNAs
Biochemistry
76
932-937
2011
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Streptococcus pyogenes
Manually annotated by BRENDA team