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adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
adenine34 in tRNAAla(AGC) + H2O
hypoxanthine34 in tRNAAla(IGC) + NH3
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
adenine34 in tRNASer(AGA) + H2O
hypoxanthine34 in tRNASer(IGA) + NH3
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
adenine34 in tRNAVal + H2O
hypoxanthine34 in tRNAVal + NH3
-
-
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNAAla(AGC) + H2O
hypoxanthine34 in tRNAAla(IGC) + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the chloroplast tRNA editing protein is specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
complementation studies show that the C-terminal domain is sufficient for cognate tRNA deamination both in vitro and in planta. TADA is exclusively involved in editing of the prokaryote-type cp-tRNAArg(ACG). No editing of cytosolic tRNAAla(AGC), tRNAVal(AAC), and tRNAThr(AGU). TADA is also not involved in editing of nucleotide A37 of tRNAAla(AGC)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
kinetic isotope effects and quantum molecular modeling are applied to solve the transition state structure of the enzyme. The key events of the tRNA editing process are facilitated by the enzyme-promoted base flip and the Glu70-mediated proton shuttle
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
-
-
-
?
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system
-
-
?
adenine34 in tRNASer(AGA) + H2O
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
adenine34 in tRNASer(AGA) + H2O
hypoxanthine34 in tRNASer(IGA) + NH3
-
-
-
?
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
-
-
-
-
?
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
-
in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA
-
-
?
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adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system
-
-
?
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
-
-
-
-
?
adenine34 in tRNAVal + H2O
hypoxanthine34 in tRNAVal + NH3
-
-
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the chloroplast tRNA editing protein is specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0000023
adenine34 in tRNASer(AGA)
pH and temperature not specified in the publication
-
0.00075 - 0.003
adenine34 in tRNAVal
-
0.00075
adenine34 in tRNAVal
-
27°C, pH 8.0, wild-type enzyme
-
0.00076
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2
-
0.0015
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2
-
0.002
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R218A
-
0.0021
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2
-
0.0025
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R217A
-
0.0028
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R219A
-
0.0028
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3
-
0.003
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme K216A
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.3 - 4.3
adenine34 in tRNAVal
-
0.3
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3
-
0.55
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2
-
0.57
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme K216A
-
0.59
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R217A
-
0.79
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R219A
-
0.86
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2
-
1
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme R218A
-
3.03
adenine34 in tRNAVal
-
27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2
-
4.27
adenine34 in tRNAVal
-
27°C, pH 8.0, wild-type enzyme
-
4.3
adenine34 in tRNAVal
-
27°C, pH 8.0, wild-type enzyme
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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F144A
mutations results in a reduction in enzymatic activity of about 800-fold
F145A
inactive mutant enzyme
R149A
mutations results in a reduction in enzymatic activity of about 800-fold
R94A
mutation substantially 220fold reduces the enzymatic activity
D64E
D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells
C-terDELTA10
-
mutation in subunit ADAT2, inactive mutant enzyme
C-terDELTA5
-
mutation in subunit ADAT2, kcat/Km is 72% compared to the wild-type value
C136A
-
kcat/Km is 13% of the wild-type value, mutation in subunit ADAT2
C136A/ADAT3 C291A
-
inactive mutant enzyme, mutation in subunit ADAT2
C139A
-
inactive mutant enzyme, mutation in subunit ADAT2
C291A
-
inactive mutant enzyme, mutation in subunit ADAT3
C294A
-
inactive mutant enzyme, mutation in subunit ADAT3
E92A/K216A/R217A/K218A/R219A
-
inactive mutant enzyme
H252A
-
inactive mutant enzyme, mutation in subunit ADAT3
H90A
-
inactive mutant enzyme, mutation in subunit ADAT2
K216A
-
mutation in subunit ADAT2, kcat/Km is 12% compared to the wild-type value
K218A
-
mutation in subunit ADAT2, kcat/Km is 24% compared to the wild-type value
R217A
-
mutation in subunit ADAT2, kcat/Km is 16% compared to the wild-type value
R219A
-
mutation in subunit ADAT2, kcat/Km is 20% compared to the wild-type value
V254E
-
inactive mutant enzyme, mutation in subunit ADAT3
V254L
-
kcat/Km is 7% of the wild-type value, mutation in subunit ADAT3
V254T
-
kcat/Km is 20% of the wild-type value, mutation in subunit ADAT3
additional information
-
deletion of the last 10 amino acids at the C terminus of TbADAT2 abolishes tRNA binding. In addition, single alanine replacements of a string of positively charged amino acids (KRKRK) lead to binding defects that correlate with losses in enzyme activity
E56A
mutation in subunit Tad2, mutant enzyme has no deaminase activity in vitro
E56A
-
mutation in subunit Tad2, mutant enzyme has no deaminase activity in vitro
-
E92A
-
inactive mutant enzyme, mutation in subunit ADAT2
E92A
-
mutation in subunit ADAT2, inactive mutant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Kuratani, M.; Ishii, R.; Bessho, Y.; Fukunaga, R.; Sengoku, T.; Shirouzu, M.; Sekine, S.; Yokoyama, S.
Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus
J. Biol. Chem.
280
16002-16008
2005
Aquifex aeolicus (O67050)
brenda
Losey, H.C.; Ruthenburg, A.J.; Verdine, G.L.
Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA
Nat. Struct. Mol. Biol.
13
153-159
2006
Staphylococcus aureus (Q99W51)
brenda
Luo, M.; Schramm, V.L.
Transition state structure of E. coli tRNA-specific adenosine deaminase
J. Am. Chem. Soc.
130
2649-2655
2008
Escherichia coli
brenda
Delannoy, E.; Le Ret, M.; Faivre-Nitschke, E.; Estavillo, G.M.; Bergdoll, M.; Taylor, N.L.; Pogson, B.J.; Small, I.; Imbault, P.; Gualberto, J.M.
Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNAArg(ACG) and is essential for efficient chloroplast translation
Plant Cell
21
2058-2071
2009
Arabidopsis thaliana (Q9S7I0), Arabidopsis thaliana
brenda
Ku, M.J.; Lee, W.H.; Nam, K.H.; Rhee, K.H.; Lee, K.S.; Kim, E.E.; Yu, M.H.; Hwang, K.Y.
Crystallization and preliminary X-ray crystallographic analysis of the tRNA-specific adenosine deaminase from Streptococcus pyogenes
Acta Crystallogr. Sect. F
61
375-357
2005
Streptococcus pyogenes (Q5XE14), Streptococcus pyogenes, Streptococcus pyogenes ATCC BAA-946 (Q5XE14)
brenda
Elias, Y.; Huang, R.H.
Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA
Biochemistry
44
12057-12065
2005
Aquifex aeolicus, Escherichia coli, Agrobacterium tumefaciens (A9CK16), Agrobacterium tumefaciens, Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
brenda
Su, A.A.; Randau, L.
A-to-I and C-to-U editing within transfer RNAs
Biochemistry
76
932-937
2011
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Streptococcus pyogenes
brenda
Wolf, J.; Gerber, A.P.; Keller, W.
tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli
EMBO J.
21
3841-3851
2002
Escherichia coli (P68398), Escherichia coli
brenda
Tsutsumi, S.; Sugiura, R.; Ma, Y.; Tokuoka, H.; Ohta, K.; Ohte, R.; Noma, A.; Suzuki, T.; Kuno, T.
Wobble inosine tRNA modification is essential to cell cycle progression in G(1)/S and G(2)/M transitions in fission yeast
J. Biol. Chem.
282
33459-33465
2007
Schizosaccharomyces pombe
brenda
Spears, J.L.; Rubio, M.A.; Gaston, K.W.; Wywial, E.; Strikoudis, A.; Bujnicki, J.M.; Papavasiliou, F.N.; Alfonzo, J.D.
A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase
J. Biol. Chem.
286
20366-20374
2011
Trypanosoma brucei
brenda
Auxilien, S.; Crain, P.F.; Trewyn, R.W.; Grosjean, H.
Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA
J. Mol. Biol.
262
437-458
1996
Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
brenda
Rubio, M.A.; Pastar, I.; Gaston, K.W.; Ragone, F.L.; Janzen, C.J.; Cross, G.A.; Papavasiliou, F.N.; Alfonzo, J.D.
An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA
Proc. Natl. Acad. Sci. USA
104
7821-7826
2007
Trypanosoma brucei
brenda
Lee, W.H.; Kim, Y.K.; Nam, K.H.; Priyadarshi, A.; Lee, E.H.; Kim, E.E.; Jeon, Y.H.; Cheong, C.; Hwang, K.Y.
Crystal structure of the tRNA-specific adenosine deaminase from Streptococcus pyogenes
Proteins
68
1016-1019
2007
Streptococcus pyogenes (Q5XE14)
brenda
Karcher, D.; Bock, R.
Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme
RNA
15
1251-1257
2009
Arabidopsis thaliana (Q9S7I0), Arabidopsis thaliana
brenda
Ragone, F.L.; Spears, J.L.; Wohlgamuth-Benedum, J.M.; Kreel, N.; Papavasiliou, F.N.; Alfonzo, J.D.
The C-terminal end of the Trypanosoma brucei editing deaminase plays a critical role in tRNA binding
RNA
17
1296-1306
2011
Trypanosoma brucei
brenda
Gerber, A.P.; Keller, W.
An adenosine deaminase that generates inosine at the wobble position of tRNAs
Science
286
1146-1149
1999
Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
brenda