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Information on EC 3.5.4.2 - adenine deaminase and Organism(s) Schizosaccharomyces pombe and UniProt Accession Q9P6I7

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.2 adenine deaminase
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: Q9P6I7 not found.
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Word Map
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
adenine deaminase, adenine aminohydrolase, adase, aah1p, adenase, pa0148, aaur1117, sgx9403e, sgx9403g, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADase
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-
-
-
adenase
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-
-
-
adenine aminase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amidines
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
adenine aminohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-68-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
-
-
-
ir
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
show the reaction diagram
3.8% of the activity of adenine
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ir
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
show the reaction diagram
2.8% of the activity of adenine
-
-
ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
show the reaction diagram
4.0% of the activity of adenine
-
-
ir
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
show the reaction diagram
2.9% of the activity of adenine
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-
ir
6-chloropurine + H2O
?
show the reaction diagram
-
-
-
-
?
6-chloropurine + H2O
hypoxanthine + HCl
show the reaction diagram
-
-
-
-
?
adenine + H2O
?
show the reaction diagram
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
kinetin + H2O
?
show the reaction diagram
N6-(2-isopentenyl)adenine + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
-
-
-
ir
6-chloropurine + H2O
?
show the reaction diagram
-
-
-
-
?
adenine + H2O
?
show the reaction diagram
-
-
-
-
?
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
kinetin + H2O
?
show the reaction diagram
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
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-
?
N6-(2-isopentenyl)adenine + H2O
?
show the reaction diagram
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
adenine
0.2 M phosphate buffer, 33°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
0.2 M phosphate buffer, 33°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33
0.2 M phosphate buffer, pH 6.7
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; wild-type 972 h
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41200
deduced from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 48000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after dissolving the freeze-dried enzyme a significant decrease od activity is observed within 3-4 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder
at 4°C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20°C in presence of 50% glycerol and at -80°C in presence of 10-20% glycerol, or as freeze-dried powder
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography
gel filtration, SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli BL21 (DE3)
expressed in Escherichia coli, pDrive, pet100/D-TOPO, pet151/D-TOPO vectors
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Abbondandolo, A.; Weyer, A.; Heslot, H.; Lambert, M.
Study of adenine aminohydrolase in the yeast, Schizosaccharomyces pombe
J. Bacteriol.
108
959-963
1971
Schizosaccharomyces pombe
Manually annotated by BRENDA team
Pospisilova, H.; Sebela, M.; Novak, O.; Frebort, I.
Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases
Biosci. Rep.
28
335-347
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P53909), Schizosaccharomyces pombe, Schizosaccharomyces pombe (Q9P6I7)
Manually annotated by BRENDA team