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Information on EC 3.5.4.2 - adenine deaminase
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3.5.4.2 adenine deaminaseSpecify your search results
Word Map
- 3.5.4.2
- purine
- hypoxanthine
- inosine
- nucleosidase
-
2.4.2.7
-
2\'-deoxycoformycin
- deaminases
-
nickase
-
phosphoribosyltransferases
- guanase
- food industry
The enzyme appears in viruses and cellular organisms
Synonyms
adenine deaminase, adenine aminohydrolase, adase, aah1p, adenase, pa0148, aaur1117, sgx9403e, sgx9403g, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
aadA
Aah1p
ADA
ADase
-
-
-
-
adenase
-
-
-
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adenine aminase
-
-
-
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adenine deaminase
KLLAOE2317
MJ1459
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
adenine + H2O = hypoxanthine + NH3
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of amidines
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
adenine aminohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9027-68-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-[(E)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.8% of the activity of adenine
-
-
?
6-[(Z)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.7% of the activity of adenine
-
-
?
2,6-diaminopurine + H2O
?
73% activity compared to adenine
-
-
?
2,6-diaminopurine + H2O
?
73% activity compared to adenine
-
-
?
2-amino-6-bromopurine + H2O
?
109% activity compared to adenine
-
-
?
2-amino-6-bromopurine + H2O
?
109% activity compared to adenine
-
-
?
6-chloropurine + H2O
?
119% activity compared to adenine
-
-
?
6-chloropurine + H2O
?
119% activity compared to adenine
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
0.7% of the activity of adenine
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ir
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
3.8% of the activity of adenine
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ir
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
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-
?
kinetin + H2O
?
hydrolysis of adenine compounds, cleavage of cytokinins into inosine and the corresponding side chain amine
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-
?
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
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-
?
kinetin + H2O
?
hydrolysis of adenine compounds, cleavage of cytokinins into inosine and the corresponding side chain amine
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-
?
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
2.6% of the activity of adenine
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ir
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
2.8% of the activity of adenine
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ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
2.5% of the activity of adenine
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ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
4.0% of the activity of adenine
-
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ir
N6-(2-isopentenyl)adenine + H2O
?
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
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-
?
N6-(2-isopentenyl)adenine + H2O
?
hydrolysis of adenine compounds, low activity towards N6-substituted adenines, partial conversion, release of N6-side chain amines
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-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
hydrolysis of adenine compounds, low activity towards N6-substituted adenines, partial conversion, release of N6-side chain amines
-
-
?
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
2.1% of the activity of adenine
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-
ir
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
2.9% of the activity of adenine
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ir
additional information
?
-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
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-
?
additional information
?
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-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
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-
?
additional information
?
-
no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP
-
-
?
additional information
?
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gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
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-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
additional information
?
-
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the protein exhibits partial amino acid sequence similarity to the CHASE domain of the cytokinin receptor, which may account for its ability to bind and hydrolyse not only kinetin, but also other cytokinins and other cytokinin derivatives, albeit at lower rates than its natural substrate adenine
-
-
?
additional information
?
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the protein exhibits partial amino acid sequence similarity to the CHASE domain of the cytokinin receptor, which may account for its ability to bind and hydrolyse not only kinetin, but also other cytokinins and other cytokinin derivatives, albeit at lower rates than its natural substrate adenine
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N6-(2-isopentenyl)adenine + H2O
?
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Fe3+
-
[FeII/FeII]-ADE is oxidized to [FeIII/FeIII]-ADE with ferricyanide with inactivation of the deaminase activity
Mn2+
Zn2+
additional information
Fe2+
-
reducing [FeIII/FeIII]-ADE with dithionite restores the deaminase activity
Fe2+
-
purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal
Mn2+
-
purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal
Zn2+
-
purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol
additional information
Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity
additional information
-
Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity
additional information
-
the apo-enzyme is prepared and reconstituted with Fe2+, Zn2+, or Mn2+. In each case, two enzyme-equivalents of metal are necessary for reconstitution of the deaminase activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one
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an inhibition of ADA activity using 100 nM is observed, an increase in cellular viability is shown when 0.03 mM is used
purine