Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5-fluorocytosine + H2O
5-fluorouracil + NH3
5-methylcytosine + H2O
thymine + NH3
-
-
-
?
cytosine + H2O
uracil + NH3
pyrimidin-2-one + H2O
(4R)-hydroxyl-3,4-dihydropyrimidine + ?
stabilization of a transition-state analogue at the active site of cytosine deaminase with importance of proton transfer from the Zn hydroxide group to Glu64 during the nucleophilic attack, quantum mechanical/molecular mechanical molecular dynamics and free energy simulations, active site structure, overview
-
-
?
5-bromocytosine + H2O
5-bromouracil + NH3
-
-
-
-
?
5-chlorocytosine + H2O
5-chlorouracil + NH3
-
-
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
5-iodocytosine + H2O
5-iodouracil + NH3
-
-
-
-
?
5-methylcytosine + H2O
thymine + NH3
-
-
-
-
?
cytosine + H2O
uracil + NH3
additional information
?
-
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
inside a cell, cytosine and 5-fluorocytosine compete for the active site of the enzyme
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
activation of the prodrug to the cytotoxic drug in cancer therapy, gene therapy with the FCY1 gene, encoding the enzyme, alone is not effective in treatement of pancreatic cancer, co-expression with uracil phosphoribosyltranferase encoding gene FUR1, as fusion enzyme, can confer sensitivity to 5-fluorouracil to some pancreatic cancer cells, but not to all, thus it might also not be an effective therapy in pancreatic cancer, overview
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
activation of the prodrug, 5-fluorouracil is an inhibitor of DNA synthesis and RNA function
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
recombinant protein of cytosine deaminase used as antigen for biopanning approach, antibody-directed enzyme-prodrug therapy (GDEPT/ADEPT), binding of specific single chain fragment variable (scFv) with cytosine deaminase does not interfere with enzyme activity
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
recombinant cytosine deaminase in gene therapy, expression and function in transgenic cell lines and xenograft tumors
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
recombinant enzyme in gene therapy, expression analysis in transgenic mice with xenograft tumors, optical imaging technologies
-
-
?
cytosine + H2O
uracil + NH3
-
-
-
?
cytosine + H2O
uracil + NH3
-
-
-
-
?
cytosine + H2O
uracil + NH3
-
-
-
?
cytosine + H2O
uracil + NH3
-
-
-
-
?
cytosine + H2O
uracil + NH3
-
-
-
?
cytosine + H2O
uracil + NH3
-
uracil binding structure at the active site, analysis by ab initio ONIOM-MD simulations showing that uracil is strongly perturbed by Ile33, which sandwiches uracil with His62, through the steric contact due to the thermal motion, detailed overview
-
?
cytosine + H2O
uracil + NH3
the enzyme is a pyrimidine salvage pathway enzyme, inside a cell, cytosine and 5-fluorocytosine compete for the active site of the enzyme
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
-
209486, 209493, 209494, 209497, 209501, 209507, 209508, 654095, 687192, 713064, 718934, 719208 -
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug 5-fluorocytosine to the anticander drug 5-fluorouracil
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
release of 5-fluorouracil is rate-limiting and may involve multiple steps
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug by the recombinant fusion enzyme, overview
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
recombinant enzyme in gene therapy in human glioblastoma cell lines Y87-1 and Y87-2
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug to the cytotoxic drug in cancer therapy by recombinant enzyme in melanoma cells, leads to increased 5-fluorouracil-mediated cell killing, overview
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug, product release is the rate-limiting step during the activation to the anticancer drug 5-fluorouracil
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
activation of the prodrug, the cytosine deaminase gene is an alternative gene for negative selection producing the toxic metabolite 5-fluorouracil, sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase, which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
-
reaction of cytosine deaminase, the uracil phosphoribosyl transferase part in a chimeric bifunctional enzyme converts 5-fluorouracil into 5-fluoro-UMP, which is a precursor for the cell toxic 5-fluoro-UTP, used for negative selection of transgenic Plasmodium falciparum parasites
-
-
?
cytosine + H2O
uracil + NH3
-
-
209484, 209485, 209486, 209487, 209488, 209489, 209492, 209493, 209494, 209495, 209497, 209501, 209506, 209507, 209508, 654095, 667664, 673932, 685615, 685937, 687192, 688612, 713064 -
-
?
cytosine + H2O
uracil + NH3
-
molecular dynamics study of the ligand release path in yeast cytosine deaminase, the active site is well protected by the C-terminal helix, residues 150158, and F-114 loop, residues 111117, substrate binding structure, overview
-
-
?
cytosine + H2O
uracil + NH3
-
the cytosine deamination proceeds via a sequential mechanism involving the protonation of the N3 of cytosine, a nucleophilic attack on C4 by the Zn-coordinated hydroxide, and the cleavage of the C4-N4 bond, combined two-layer ONIOM quantum chemical-molecular dynamics study, method, development, overview
-
-
?
additional information
?
-
the rate-determining step of the reaction concerns the breaking of the product-metal binding state and has a barrier of about 18 kcal/mol. The protonation of cytosine by Glu64 in the substrate binding stage as well as the product transport step are also important and influence the whole catalytic efficiency, due to their considerable barriers (12-13 kcal/mol). The driving force for the overall enzymatic reaction likely comes from the reactant delivery to the active site of the protein
-
-
?
additional information
?
-
-
the rate-determining step of the reaction concerns the breaking of the product-metal binding state and has a barrier of about 18 kcal/mol. The protonation of cytosine by Glu64 in the substrate binding stage as well as the product transport step are also important and influence the whole catalytic efficiency, due to their considerable barriers (12-13 kcal/mol). The driving force for the overall enzymatic reaction likely comes from the reactant delivery to the active site of the protein
-
-
?
additional information
?
-
-
cytosine deaminase gene therapy combined with radiation treatment in breast cancer is evaluated in a 4T1murine breast carcinoma model, overview
-
-
?
additional information
?
-
-
cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cell lines Y87-1 and Y87-2 in vitro, overview
-
-
?
additional information
?
-
-
the cytosine deaminase gene acts as a suicide gene by catalyzing the conversion of 5-fluorocytosine to cytotoxic 5-fluorouracil, fusion of the HSV-1 tegument protein vp22 to cytosine deaminase confers enhanced bystander effect and increased therapeutic benefit in cancer gene therapy of gliosarcoma cells and murine 9L tumors, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A23L/D92E/V108I/I140L
construction of superimposed mutants by combining randomly generated single nucleotide mutants with the triple mutant, the superimposed mutant does not show enhanced activity with 5-fluorouracil and negates the effect introduced by the triple substitutions
A23L/I140L
site-directed mutagenesis, the mutant display elevated unfolding temperatures in denaturation experiments and increased half-lives of catalytic activity at elevated temperatures, the mutant cells show an about 30% reduced sensitivity to 5-fluorouracil compared to the wild-type cells
A23L/M93L/V108I/I140L
construction of superimposed mutants by combining randomly generated single nucleotide mutants with the triple mutant, the superimposed mutant does not show enhanced activity with 5-fluorouracil and negates the effect introduced by the triple substitutions
A23L/V108I/I140L
site-directed mutagenesis, display elevated unfolding temperatures in denaturation experiments and increased half-lives of catalytic activity at elevated temperatures, the mutant cells show an about 50% reduced sensitivity to 5-fluorouracil compared to the wild-type cells
A23L/V108I/I140L/I98L
construction of superimposed mutants by combining randomly generated single nucleotide mutants with the triple mutant, the superimposed mutant does not show enhanced activity with 5-fluorouracil and negates the effect introduced by the triple substitutions
D92E
random mutagenesis, the mutation is located at the enzyme's dimer interface, the mutant shows increased thermal stability with elevated Tm values and increased activity half-life compared to the wild-type enzyme, the mutant cells show an about 30% reduced sensitivity to 5-fluorouracil compared to the wild-type cells
E64D
-
the effect of E64D mutation are slightly milder than the E64A mutation
E64A
-
the mutant lacks enzyme activity
E64A
-
E64A mutation causes a decrease in kcat of 5 orders of magnitude and an increase in Km of 2 orders of magnitude, resulting in a decrease in kcat/Km of 8 orders of magnitude
additional information
construction of a fusion protein of yeast cytosine deaminase with yeast uracil phosphoribosyltransferase
additional information
-
construction of a fusion protein of yeast cytosine deaminase with yeast uracil phosphoribosyltransferase
additional information
to create enzyme variants with increased activity to 5-fluorocytosine, 11 codons within the most conserved region of the enzyme, T83, L84, Y85, T86, L88, S89, D92, M93, T95, G96, and I98 are subjected to regiospecific, partially randomizing mutagenesis, Absolutely conserved residues, T87, P90, C91, and C94, within this same region, assumed to be critical for activity, are omitted from randomization, overview
additional information
-
to create enzyme variants with increased activity to 5-fluorocytosine, 11 codons within the most conserved region of the enzyme, T83, L84, Y85, T86, L88, S89, D92, M93, T95, G96, and I98 are subjected to regiospecific, partially randomizing mutagenesis, Absolutely conserved residues, T87, P90, C91, and C94, within this same region, assumed to be critical for activity, are omitted from randomization, overview
additional information
construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages
additional information
-
co-expression of the uracil phosphoribosyltransferase gene with a chimeric truncated human nerve growth factor receptor/cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosine, for positive selection, overview, construct NG/CDiU expressing UPRT and NG/CD, using a bicistronic message, provides the greatest UPRT activity and killing, reducing the lethal dose of 5-FC sufficient to eradicate 90% of cells
additional information
-
construction of a chimeric bifuntional enzyme comprising cytosine deaminase and uracil phosphoribosyl transferase activities, overview
additional information
-
construction of a fusion gene consisting of the SV40 minimal promoter, nine copies of a hypoxia-responsive element, and the yeast CD gene
additional information
-
construction of a fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene
additional information
-
construction of a His6-tagged fusion protein using the yeast cytosine deaminase and a single chain fragment variable human antibody specific for carcinoembryonic antigen, CEA, cell surfaces under control of the lac promoter, the chimeric enzyme shows high affinity for binding to Mel P5 and LoVo melanoma cells and improves tumor-selective prodrug activation, overview
additional information
-
fusion of the HSV-1 tegument protein vp22, as full-length protein and as truncated protein lacking the trafficking residues, to cytosine deaminase and stable expression in 9L tumors confers enhanced bystander effect and increased therapeutic benefit in cancer gene therapy, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ipata, P.L.; Marmocchi, F.; Magni, G.; Felicioli, R.; Polidoro, G.
Baker's yeast cytosine deaminase. Some enzymic properties and allosteric inhibition by nucleosides and nucleotides
Biochemistry
10
4270-4276
1971
Saccharomyces cerevisiae, Escherichia coli
brenda
Balestreri, E.; Felicioli, R.A.; Ipata, P.L.
The effect of pH on the alosteric properties of baker's yeast cytosine deaminase
Biochim. Biophys. Acta
293
443-448
1973
Saccharomyces cerevisiae
-
brenda
Holldorf, A.W.
Cytosine
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
3
1964-1966
1974
Saccharomyces cerevisiae
-
brenda
Sakai, T.; Yu, T.; Tabe, H.; Omata, S.
Purification of cytosine deaminase from Serratia marcescens
Agric. Biol. Chem.
39
1623-1629
1975
Saccharomyces cerevisiae, Escherichia coli, Serratia marcescens
-
brenda
Sakai, T.; Yu, T.; Taniguchi, K.; Omata, S.
Purification of cytosine deaminase from Pseudomonas aureofaciens
Agric. Biol. Chem.
39
2015-2020
1975
Klebsiella aerogenes, Enterobacter cloacae, Alcaligenes faecalis, Bacillus subtilis, Saccharomyces cerevisiae, Citrobacter freundii, Escherichia coli, Micrococcus flavus, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas chlororaphis subsp. aureofaciens, Pseudomonas chlororaphis, Pseudomonas cruciviae, Pseudomonas fluorescens, Pseudomonas fragi, Pseudomonas schuylkilliensis, Ralstonia solanacearum, Pseudomonas coronafaciens pv. striafaciens, Pseudomonas taetrolens, Pseudomonas trifolii, Serratia marcescens, Serratia polymuthicum, Alcaligenes faecalis type S, Serratia marcescens type S, Enterobacter cloacae IAM 1221, Pseudomonas schuylkilliensis type S, Escherichia coli K12 AKU 0005, Pseudomonas chlororaphis type S, Proteus vulgaris type S, Pseudomonas aeruginosa type S, Pseudomonas putida type S, Proteus mirabilis type S, Pseudomonas trifolii type S, Enterobacter cloacae type S, Alcaligenes faecalis AKU 0101, Serratia polymuthicum AKU 0062, Pseudomonas cruciviae type S, Micrococcus flavus AKU 0502, Klebsiella aerogenes type S, Ralstonia solanacearum type S
-
brenda
Yu, T.; Sakai, T.; Omata, S.
Kinetic properties of cytosine deaminase from Serratia marcescens
Agric. Biol. Chem.
40
543-549
1976
Saccharomyces cerevisiae, Escherichia coli, Pseudomonas chlororaphis subsp. aureofaciens, Serratia marcescens
-
brenda
Ipata, P.L.; Cercignani, G.
Cytosine and cytidine deaminase from yeast
Methods Enzymol.
51
394-401
1978
Saccharomyces cerevisiae
brenda
West, T.P.; Shanley, M.S.; O'Donovan, G.A.
Purification and some properties of cytosine deaminase from Salmonella typhimurium
Biochim. Biophys. Acta
791
251-258
1982
Saccharomyces cerevisiae, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2
brenda
West, T.P.
Effect of pyrophosphate and orotidine monophosphate on cytosine deaminase regulatory properties
Experientia
41
1563-1564
1988
Saccharomyces cerevisiae, Pseudomonas chlororaphis subsp. aureofaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Salmonella enterica subsp. enterica serovar Typhimurium HD11-AE2
brenda
Kornblatt, M.J.; Tee, O.S.
Inhibition of yeast cytosine deaminase by 5-bromo-2-pyrimidinone and its covalent hydrate
Eur. J. Biochem.
756
297-300
1986
Saccharomyces cerevisiae
brenda
Katsuragi, T.; Sakai, T.; Matsumoto, K.; Tonomura, K.
Cytosine deaminase from Escherichia coli-production, purification and some characteristics
Agric. Biol. Chem.
50
1721-1730
1986
Saccharomyces cerevisiae, Escherichia coli, Pseudomonas chlororaphis subsp. aureofaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens
-
brenda
Yu, T.S.; Kim, J.K.; Kasuragi, T.; Sakai, T.; Tonomura, K.
Purification and properties of cytosine deaminase from Aspergillus fumigatus
J. Ferment. Bioeng.
72
266-269
1991
Arthrobacter sp., Aspergillus fumigatus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Arthrobacter sp. JH-13, Aspergillus fumigatus IFO5840
-
brenda
Erbs, P.; Exinger, F.; Jund, R.
Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans
Curr. Genet.
31
1-6
1997
Aspergillus fumigatus, Saccharomyces cerevisiae, Candida albicans, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Aspergillus fumigatus IFO5840
brenda
Yu, T.S.; Kim, J.; Kim, H.S.
Chemical modification of cytosine deaminase from Aspergillus fumigatus
J. Microbiol.
36
39-42
1998
Aspergillus fumigatus, Saccharomyces cerevisiae, Chromobacterium violaceum, Escherichia coli, Pseudomonas chlororaphis subsp. aureofaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Chromobacterium violaceum YK 391
-
brenda
Kim, T.H.; Yu, T.S.
Chemical modification of extracellular cytosine deaminase from Chromobacterium violaceum YK 391
J. Microbiol. Biotechnol.
8
581-587
1998
Arthrobacter sp., Aspergillus fumigatus, Paenibacillus polymyxa, Saccharomyces cerevisiae, Chromobacterium violaceum, Escherichia coli, Pseudomonas chlororaphis subsp. aureofaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Chromobacterium violaceum YK 391
-
brenda
Hsu, Y.H.; Hu, C.Y.; Lin, J.J.; Liaw, S.H.
Crystallization and preliminary crystallographic analysis of yeast cytosine deaminase
Acta Crystallogr. Sect. D
59
950-952
2003
Saccharomyces cerevisiae
brenda
Ko, T.P.; Lin, J.J.; Hu, C.Y.; Hsu, Y.H.; Wang, A.H.; Liaw, S.H.
Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution
J. Biol. Chem.
278
19111-19117
2003
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Ireton, G.C.; Stoddard, B.L.
Microseed matrix screening to improve crystals of yeast cytosine deaminase. [Erratum to document cited in CA141:019564]
Acta Crystallogr. Sect. D
D60
801
2004
Saccharomyces cerevisiae
-
brenda
Yao, L.; Li, Y.; Wu, Y.; Liu, A.; Yan, H.
Product release is rate-limiting in the activation of the prodrug 5-fluorocytosine by yeast cytosine deaminase
Biochemistry
44
5940-5947
2005
Saccharomyces cerevisiae
brenda
Yao, L.; Sklenak, S.; Yan, H.; Cukier, R.I.
A molecular dynamics exploration of the catalytic mechanism of yeast cytosine deaminase
J. Phys. Chem. B
109
7500-7510
2005
Saccharomyces cerevisiae
brenda
O'Brien, T.A.; Tuong, D.T.; Basso, L.M.; McIvor, R.S.; Orchard, P.J.
Coexpression of the uracil phosphoribosyltransferase gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosine
Hum. Gene Ther.
17
518-530
2006
Saccharomyces cerevisiae
brenda
Xing, L.; Deng, X.; Kotedia, K.; Ackerstaff, E.; Ponomarev, V.; Clifton Ling, C.; Koutcher, J.A.; Li, G.C.
Non-invasive molecular and functional imaging of cytosine deaminase and uracil phosphoribosyltransferase fused with red fluorescence protein
Acta Oncol.
47
1211-1220
2008
Saccharomyces cerevisiae (Q12178)
brenda
Yao, L.; Yan, H.; Cukier, R.I.
A molecular dynamics study of the ligand release path in yeast cytosine deaminase
Biophys. J.
92
2301-2310
2007
Saccharomyces cerevisiae
brenda
Ou-Yang, F.; Lan, K.L.; Chen, C.T.; Liu, J.C.; Weng, C.L.; Chou, C.K.; Xie, X.; Hung, J.Y.; Wei, Y.; Hortobagyi, G.N.; Hung, M.C.
Endostatin-cytosine deaminase fusion protein suppresses tumor growth by targeting neovascular endothelial cells
Cancer Res.
66
378-384
2006
Saccharomyces cerevisiae
brenda
Lee, K.C.; Hamstra, D.A.; Bullarayasamudram, S.; Bhojani, M.S.; Moffat, B.A.; Dornfeld, K.J.; Ross, B.D.; Rehemtulla, A.
Fusion of the HSV-1 tegument protein vp22 to cytosine deaminase confers enhanced bystander effect and increased therapeutic benefit
Gene Ther.
13
127-137
2006
Saccharomyces cerevisiae
brenda
Zamboni, S.; Mallano, A.; Flego, M.; Ascione, A.; Dupuis, M.L.; Gellini, M.; Barca, S.; Cianfriglia, M.
Genetic construction, expression, and characterization of a single chain anti-CEA antibody fused to cytosine deaminase from yeast
Int. J. Oncol.
32
1245-1251
2008
Saccharomyces cerevisiae
brenda
Chen, J.K.; Hu, L.J.; Wang, D.; Lamborn, K.R.; Deen, D.F.
Cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cells in vitro
Int. J. Radiat. Oncol. Biol. Phys.
67
1538-1547
2007
Saccharomyces cerevisiae
brenda
Matsubara, T.; Dupuis, M.; Aida, M.
An insight into the environmental effects of the pocket of the active site of the enzyme. Ab initio ONIOM-molecular dynamics (MD) study on cytosine deaminase
J. Comput. Chem.
29
458-465
2008
Saccharomyces cerevisiae (Q12178)
brenda
Stolworthy, T.S.; Korkegian, A.M.; Willmon, C.L.; Ardiani, A.; Cundiff, J.; Stoddard, B.L.; Black, M.E.
Yeast cytosine deaminase mutants with increased thermostability impart sensitivity to 5-fluorocytosine
J. Mol. Biol.
377
854-869
2008
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Yao, L.; Yan, H.; Cukier, R.I.
A combined ONIOM quantum chemical-molecular dynamics study of zinc-uracil bond breaking in yeast cytosine deaminase
J. Phys. Chem. B
110
26320-26326
2006
Saccharomyces cerevisiae
brenda
Xu, Q.; Guo, H.; Gorin, A.; Guo, H.
Stabilization of a transition-state analogue at the active site of yeast cytosine deaminase: importance of proton transfers
J. Phys. Chem. B
111
6501-6506
2007
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Maier, A.G.; Braks, J.A.; Waters, A.P.; Cowman, A.F.
Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination
Mol. Biochem. Parasitol.
150
118-121
2006
Saccharomyces cerevisiae
brenda
Fogar, P.; Navaglia, F.; Basso, D.; Greco, E.; Zambon, C.F.; Fadi, E.; Falda, A.; Stranges, A.; Vannozzi, F.; Danesi, R.; Pedrazzoli, S.; Plebani, M.
Suicide gene therapy with the yeast fusion gene cytosine deaminase/uracil phosphoribosyltransferase is not enough for pancreatic cancer
Pancreas
35
224-231
2007
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Mallano, A.; Zamboni, S.; Carpinelli, G.; Santoro, F.; Flego, M.; Ascione, A.; Gellini, M.; Tombesi, M.; Podo, F.; Cianfriglia, M.
Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from yeast
BMC Biotechnol.
8
68
2008
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Kucerova, L.; Matuskova, M.; Pastorakova, A.; Tyciakova, S.; Jakubikova, J.; Bohovic, R.; Altanerova, V.; Altaner, C.
Cytosine deaminase expressing human mesenchymal stem cells mediated tumour regression in melanoma bearing mice
J. Gene Med.
10
1071-1082
2008
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae
brenda
Park, J.I.; Cao, L.; Platt, V.M.; Huang, Z.; Stull, R.A.; Dy, E.E.; Sperinde, J.J.; Yokoyama, J.S.; Szoka, F.C.
Antitumor therapy mediated by 5-fluorocytosine and a recombinant fusion protein containing TSG-6 hyaluronan binding domain and yeast cytosine deaminase
Mol. Pharm.
6
801-812
2009
Saccharomyces cerevisiae
brenda
Wang, J.; Sklenak, S.; Liu, A.; Felczak, K.; Wu, Y.; Li, Y.; Yan, H.
Role of glutamate 64 in the activation of the prodrug 5-fluorocytosine by yeast cytosine deaminase
Biochemistry
51
475-486
2012
Saccharomyces cerevisiae
brenda
Kucerova, L.; Matuskova, M.; Hlubinova, K.; Bohovic, R.; Feketeova, L.; Janega, P.; Babal, P.; Poturnajova, M.
Bystander cytotoxicity in human medullary thyroid carcinoma cells mediated by fusion yeast cytosine deaminase and 5-fluorocytosine
Cancer Lett.
311
101-112
2011
Saccharomyces cerevisiae
brenda
Zhao, Y.; She, N.; Zhang, X.; Wang, C.; Mo, Y.
Product release mechanism and the complete enzyme catalysis cycle in yeast cytosine deaminase (yCD) A computational study
Biochim. Biophys. Acta
1865
1020-1029
2017
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q12178)
brenda
Blacklock, K.M.; Yachnin, B.J.; Woolley, G.A.; Khare, S.D.
Computational design of a photocontrolled cytosine deaminase
J. Am. Chem. Soc.
140
14-17
2018
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q12178)
brenda
Leonhardt, N.; Divol, F.; Chiarenza, S.; Deschamps, S.; Renaud, J.; Giacalone, C.; Laurent, N.; Berthome, R.; Peret, B.
Tissue-specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology
Plant J.
101
731-741
2019
Saccharomyces cerevisiae (Q12178), Saccharomyces cerevisiae ATCC 204508 (Q12178)
brenda