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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
formiminoglutamase, formiminoglutamate hydrolase,
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N-formimino-L-glutamate formiminohydrolase
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formimidoyl-L-glutamate formiminohydrolase
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formiminoglutamase
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Formiminoglutamate hydrolase
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formylglutamate amidohydrolase
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N-formimino-L-glutamate formiminohydrolase
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N-formiminoglutamate hydrolase
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hydrolysis of C-N bond of an amidino group
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N-formimidoyl-L-glutamate formimidoylhydrolase
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N-formimidoyl-L-glutamate + H2O
L-glutamate + formamide
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additional information
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additional information
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no activity with L-arginine, agmatine, L-acetylarginine, alpha-guanidinoglutaric acid, L-homoarginine, L-2-amino-3-guanidinopropionic acid, 3-guanidinopropionic acid, and 4-guanidinobutyric acid
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additional information
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no activity with L-arginine, agmatine, L-acetylarginine, alpha-guanidinoglutaric acid, L-homoarginine, L-2-amino-3-guanidinopropionic acid, 3-guanidinopropionic acid, and 4-guanidinobutyric acid
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N-formimidoyl-L-glutamate + H2O
L-glutamate + formamide
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9 - 160
N-formimidoyl-L-glutamate
9
N-formimidoyl-L-glutamate
mutant enzyme R144E, at pH 8.5 and 22°C
20
N-formimidoyl-L-glutamate
mutant enzyme N114H, at pH 8.5 and 22°C
40
N-formimidoyl-L-glutamate
wild type enzyme, at pH 8.5 and 22°C
90
N-formimidoyl-L-glutamate
mutant enzyme R144A, at pH 8.5 and 22°C
160
N-formimidoyl-L-glutamate
mutant enzyme R144K, at pH 8.5 and 22°C
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0.05 - 200
N-formimidoyl-L-glutamate
0.05
N-formimidoyl-L-glutamate
mutant enzyme R144E, at pH 8.5 and 22°C
3.2
N-formimidoyl-L-glutamate
mutant enzyme R144A, at pH 8.5 and 22°C
80
N-formimidoyl-L-glutamate
mutant enzyme N114H, at pH 8.5 and 22°C
190
N-formimidoyl-L-glutamate
mutant enzyme R144K, at pH 8.5 and 22°C
200
N-formimidoyl-L-glutamate
wild type enzyme, at pH 8.5 and 22°C
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0.0052 - 5.2
N-formimidoyl-L-glutamate
0.0052
N-formimidoyl-L-glutamate
mutant enzyme R144E, at pH 8.5 and 22°C
0.037
N-formimidoyl-L-glutamate
mutant enzyme R144A, at pH 8.5 and 22°C
1.2
N-formimidoyl-L-glutamate
mutant enzyme R144K, at pH 8.5 and 22°C
3.4
N-formimidoyl-L-glutamate
mutant enzyme N114H, at pH 8.5 and 22°C
5.2
N-formimidoyl-L-glutamate
wild type enzyme, at pH 8.5 and 22°C
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UniProt
brenda
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Q4DSA0_TRYCC
Trypanosoma cruzi (strain CL Brener)
308
0
33556
TrEMBL
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hanging drop vapor diffusion method, using 25% PEG (w/v) 3350, 0.1 M sodium acetate (pH 4.6)
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N114H
the mutant exhibits 35% loss of catalytic efficiency compared to the wild type enzyme
R144A
the mutant exhibits 99.3% loss of catalytic efficiency compared to the wild type enzyme
R144E
the mutant exhibits 99.9% loss of catalytic efficiency compared to the wild type enzyme
R144K
the mutant exhibits 77% loss of catalytic efficiency compared to the wild type enzyme
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Talon column chromatography and Q-HP anion exchange column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Hai, Y.; Dugery, R.J.; Healy, D.; Christianson, D.W.
Formiminoglutamase from Trypanosoma cruzi is an arginase-like manganese metalloenzyme
Biochemistry
52
9294-9309
2013
Trypanosoma cruzi (Q4DSA0), Trypanosoma cruzi
brenda