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IUBMB CommentsOther diguanidinoalkanes with 3 to 10 methylene groups can also act, but more slowly.
The expected taxonomic range for this enzyme is: Pseudomonas putida
Synonyms
diguanidinobutane amidinohydrolase,
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diguanidinobutane amidinohydrolase
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1,4-diguanidinobutane + H2O = agmatine + urea
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hydrolysis of linear amidines
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1,4-diguanidinobutane amidinohydrolase
Other diguanidinoalkanes with 3 to 10 methylene groups can also act, but more slowly.
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1,3-diguanidinopropane + H2O
?
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-
-
-
?
1,4-diguanidinobutane + H2O
agmatine + urea
1,5-diguanidinopentane + H2O
?
-
-
-
-
?
1,6-diguanidinohexane + H2O
?
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-
-
-
?
1,7-diguanidinoheptane + H2O
?
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-
-
-
?
1,8-diguanidinooctane + H2O
?
-
-
-
-
?
1-amino-5-guanidinopentane + H2O
1,5-diaminopentane + urea
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-
-
-
?
1-amino-6-guanidinohexane + H2O
1,6-diaminohexane + urea
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-
-
-
?
1-amino-7-guanidinoheptane + H2O
1,7-diaminoheptane + urea
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-
-
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?
agmatine + H2O
putrescine + urea
diguanidinoalkanoate + H2O
aminoalkylguanidine + urea
1,4-diguanidinobutane + H2O
agmatine + urea
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?
1,4-diguanidinobutane + H2O
agmatine + urea
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?
agmatine + H2O
putrescine + urea
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low activity towards agmatine
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?
agmatine + H2O
putrescine + urea
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low activity towards agmatine
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?
diguanidinoalkanoate + H2O
aminoalkylguanidine + urea
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utilization of alkanoates with 3-10 methylen groups
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?
diguanidinoalkanoate + H2O
aminoalkylguanidine + urea
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utilization of alkanoates with 3-10 methylen groups
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?
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1,4-diguanidinobutane + H2O
agmatine + urea
1,4-diguanidinobutane + H2O
agmatine + urea
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?
1,4-diguanidinobutane + H2O
agmatine + urea
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?
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Co2+
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required for full activity
Ni2+
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required for full activity
Mn2+
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required for full activity, no inactivation by incubation with 10 mM EDTA
Mn2+
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two ions per subunit
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1,10-Diaminodecane
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41% inhibition at concentration of 3.0 mM
1,7-Diaminoheptane
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22% inhibition at concentration of 5.0 mM
1,8-diaminooctane
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34% inhibition at concentration of 3.0 mM
1,9-diaminononane
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38% inhibition at concentration of 3.0 mM
1-amino-5-guanidinopentane
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29% inhibition at concentration of 5.0 mM
5-Guanidinovalerate
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10% inhibition at concentration of 5.0 mM
agmatine
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28% inhibition at concentration of 5.0 mM
DTNB
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11% inhibition at concentration of 5.0 mM
N-carbamoylagmatine
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62% inhibition at concentration of 5.0 mM
p-chloromercuribenzoate
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complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
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0.91
1,3-diguanidinopropane
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0.65 - 0.7
1,4-Diguanidinobutane
0.5
1,5-Diguanidinopentane
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0.33
1,6-Diguanidinohexane
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-
0.24
1,7-Diguanidinoheptane
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-
0.23
1,8-Diguanidinooctane
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2.5
1-amino-5-guanidinopentane
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-
1.3
1-amino-6-guanidinohexane
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1
1-amino-7-guanidinoheptane
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-
0.65
1,4-Diguanidinobutane
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0.7
1,4-Diguanidinobutane
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-
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1330
1,4-Diguanidinobutane
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per subunit
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10 - 11
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10 - 11
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in sodium carbonate and piperazine hydrochloric buffers
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brenda
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tetramer
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4*38000 Da, SDS-PAGE
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10
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stable in piperazine hydrochloride
209444
7 - 10
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most stable
209443
7 - 8
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stable in potassium phosphate
209444
7 - 9
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stable in TRIS-HCl
209444
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30
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no loss of activity within 2 h in presence of 10 mM EDTA
50
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unstable on heating for 15 min
60
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complete inactivation in presence and absence of Mn2+
40
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stable on heating for 15 min
40
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stable on heating in presence of Mn2+
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synthesis
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preparation of pure agmatine sulfate
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Yorifuji, T.; Kaneoke, M.; Shimizu, E.; Shiota, K.; Matsuo, R.
Degradation of alpha,omega-diguanidinoalkanoates and a novel enzyme, diguanidinobutane amidohydrolase in Pseudomonas putida
Agric. Biol. Chem.
53
3003-3009
1989
Pseudomonas putida
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brenda
Shoeb, S.M.; Kaneoke, M.; Shimizu, E.; Yorifuji, T.
Diguanidinobutanase of Pseudomonas putida and enzymatic preparation of agmatine from 1,4-diguanidinobutanate
Appl. Microbiol. Biotechnol.
44
94-99
1995
Pseudomonas putida
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brenda
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3.5.3.20 diguanidinobutanase
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