We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The taxonomic range for the selected organisms is: Bos taurus The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
ddah1, dimethylarginine dimethylaminohydrolase, ddah2, ddah-1, ddah-2, dimethylaminohydrolase, dimethylarginine dimethylaminohydrolase 1, dimethylargininase, human ddah-1, ddah-i,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimethylarginine dimethylaminohydrolase
-
Dimethylargininase
-
-
-
-
dimethylarginine dimethylaminohydrolase
dimethylarginine dimethylaminohydrolase 1
-
-
Nomega,Nomega-dimethyl-L-arginine dimethylaminohydrolase-1
-
DDAH-1
-
-
dimethylarginine dimethylaminohydrolase
-
-
-
-
dimethylarginine dimethylaminohydrolase
-
-
dimethylarginine dimethylaminohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of linear amidines
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Nomega,Nomega'-dimethyl-L-arginine dimethylamidohydrolase
Also acts on Nomega-methyl-L-arginine.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimethyl-L-arginine + H2O
L-citrulline + dimethylamine
-
-
-
?
Ngamma-monomethyl-L-arginine + H2O
L-citrulline + methylamine
-
-
-
?
S-methyl-L-thiocitrulline + H2O
L-citrulline + methanethiol
-
-
-
?
dimethyl-L-arginine + H2O
L-citrulline + dimethylamine
-
-
-
?
N,N-dimethyl-L-arginine + H2O
dimethylamine + L-citrulline
-
-
-
?
NG,NG-dimethyl-L-arginine + H2O
dimethylamine + L-citrulline
NG-monomethyl-L-arginine + H2O
citrulline + methylamine
-
-
-
-
?
Ngamma-monomethyl-L-arginine + H2O
L-citrulline + methylamine
-
-
-
?
S-methyl-L-thiocitrulline + H2O
L-citrulline + methanethiol
-
-
-
?
NG,NG-dimethyl-L-arginine + H2O
dimethylamine + L-citrulline
-
-
-
-
?
NG,NG-dimethyl-L-arginine + H2O
dimethylamine + L-citrulline
-
enzyme is involved in regulation of the levels of the natural occuring free arginine derivatives L-Nomega,Nomega-dimethylarginine and L-Nomega-methylarginine, which are reversible inhibitors of nitric oxide synthase
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NG,NG-dimethyl-L-arginine + H2O
dimethylamine + L-citrulline
-
enzyme is involved in regulation of the levels of the natural occuring free arginine derivatives L-Nomega,Nomega-dimethylarginine and L-Nomega-methylarginine, which are reversible inhibitors of nitric oxide synthase
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Zn2+
-
contains one tightly bound zinc ion
Zn2+
Zn2+ binding to DDAH-1 protects the enzyme from S-nitrosylation by free nitric oxide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N-nitro-L-arginine methylester
1mM, 1 h, 47.1% relative activity
Zn2+
20 microM, 40% relative activity
2-(N,N-dimethylamino)-diazenolate-2-oxide
-
S-nitrosylation of apo-enzyme, holo-enzyme resists, reaction with two of five Cys-residues
methylamine
-
10 mM, 80% of activity
N-nitro-L-arginine methylester
1 mM, 1 h, 47.1% relative activity
S-nitroso-L-cysteine
9-15% inhibition of the zinc-free enzyme at 0.5 mM, S-nitrosylation of the active site Cys273 in DDAH-1
additional information
5 mM of L-cysteine, L-homocysteine, glutathione, dithiothreitol, or Tris(2-carboxyethyl)phosphine-HCl for 30 min at 37°C cannot restore inhibited activity, suggesting that the covalent product N-thiosulfoximide will remain stable under the reducing conditions present in the cytosol
-
S-nitroso-L-homocysteine
-
S-nitroso-L-homocysteine
96% inhibition of the zinc-free enzyme at 0.5 mM, leads to the unique formation of an N-thiosulfoximide
Zn2+
-
Zn2+
-
besides the tightly bound zinc, additional Zn2+ inhibit competitively
Zn2+
-
endogenous inhibitor, regulatory role
Zn2+
-
one mol per mol of enzyme, apo-enzyme is active, holoenzyme is activated by removal of Zn2+
Zn2+
20 microM, 40% relative activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-histidine
-
activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine
histamine
-
activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine
imidazole
-
activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine
L-histidine
-
activating ability in decreasing order: histamine, imidazole, L-histidine, D-histidine
additional information
-
type of buffer dramatically influences enzyme activity, activity in imidazole/HCl buffer is about 25% higher than in phosphate buffer
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.18
dimethyl-L-arginine
-
0.18
dimethyl-L-arginine
-
1.31
NG,NG-dimethyl-L-arginine
-
-
1.6
NG-monomethyl-L-arginine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.27
NG,NG-dimethyl-L-arginine
-
-
0.34
NG-monomethyl-L-arginine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.69
S-nitroso-L-homocysteine
in the presence of 5 mM EDTA, at pH 7.3 and 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.075
S-nitroso-L-homocysteine
Bos taurus
in 50 mM HEPES/NaOH (pH 7.3), 150 mM KCl, and 5 mM EDTA for 30 min at 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.8
-
imidazole/HCl buffer
additional information
-
type of buffer dramatically influences enzymatic activity
6.2
-
phosphate buffer
6.2
-
hydrolysis of NG,NG-dimethyl-L-arginine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
isoform DDAH-2
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
brenda
aortic endothelial cell
brenda
-
brenda
-
brenda
-
brenda
-
brenda
-
-
brenda
aortic endothelial cell
brenda
-
brenda
-
-
brenda
-
brenda
-
brenda
-
brenda
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
overexpression of DDAH-2 increases endothelial nitric oxide by 18%. Small interfering RNA-mediated down-regulation of DDAH-2 reduces nitric oxide bioavailability by 57%. L-Arg and asymmetric dimethylarginine are unchanged in the DDAH-2-silenced cells, and L-Arg supplementation has no effect on nitric oxide
physiological function
overexpression of DDAH-1 increases endothelial nitric oxide by 24%. Small interfering RNA-mediated down-regulation of DDAH-1 reduces nitric oxide bioavailability by 27%. The reduction in nitric oxide production following DDAH-1 gene silencing is associated with a 48% reduction in L-Arg/asymmetric dimethylarginine and is partially restored with L-Arg supplementation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DDAH2_BOVIN
285
0
29780
Swiss-Prot
other Location (Reliability: 2 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
31200
electrospray ionization mass spectrometry
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
by sitting drop vapor diffusion, at 1.08-2.0 A resolution and at pH 6.3 and pH 9.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50
-
2 h, holo-enzyme retains ca. 56% of the original activity, no residual activity of the apoenzyme. Thermal denaturation of both protein forms is irreversible
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Zn2+ has a structure-stabilizing role
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
anion exchange chromatography and gel filtration chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
method for reversible preparation of metal-free and metal-containing enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
medicine
influence on atherosclerosis in experimental models by increased ADMA concentrations and reduced NO synthesis
medicine
-
expression and secretion of the vascular endothelial growth factor is not increased in DDAH1-transfected cells
medicine
structure-based development of specific DDAH-1 inhibitors that might be useful in the therapeutic treatment of NOS dysfunction-related diseases
medicine
influence on atherosclerosis in experimental models by increased ADMA concentrations and reduced NO synthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Bogumil, R.; Knipp, M.; Fundel, S.M.; Vasak, M.
Characterization of dimethylargininase from bovine brain: evidence for a zinc binding site
Biochemistry
37
4791-4798
1998
Bos taurus
brenda
Knipp, M.; Charnock, J.M.; Garner, C.D.; Vasak, M.
Structural and functional characterization of the Zn(II) site in dimethylargininase-1 (DDAH-1) from bovine brain. Zn(II) release activates DDAH-1
J. Biol. Chem.
276
40449-40456
2001
Bos taurus
brenda
Knipp, M.; Braun, O.; Gehrig, P.M.; Sack, R.; Vasak, M.
Zn(II)-free dimethylargininase-1 (DDAH-1) is inhibited upon specific Cys-S-nitrosylation
J. Biol. Chem.
278
3410-3416
2003
Bos taurus
brenda
Hasegawa, K.; Wakino, S.; Tanaka, T.; Kimoto, M.; Tatematsu, S.; Kanda, T.; Yoshioka, K.; Homma, K.; Sugano, N.; Kurabayashi, M.; Saruta, T.; Hayashi, K.
Dimethylarginine dimethylaminohydrolase 2 increases vascular endothelial growth factor expression through Sp1 transcription factor in endothelial cells
Arterioscler. Thromb. Vasc. Biol.
26
1488-1494
2006
Bos taurus, Homo sapiens
brenda
Frey, D.; Braun, O.; Briand, C.; Vak, M.; Grtter, M.G.
Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: a basis for the design of specific inhibitors
Structure
14
901-911
2006
Bos taurus (P56965), Bos taurus
brenda
Braun, O.; Knipp, M.; Chesnov, S.; Vasak, M.
Specific reactions of S-nitrosothiols with cysteine hydrolases: A comparative study between dimethylargininase-1 and CTP synthetase
Protein Sci.
16
1522-1534
2007
Bos taurus (P56965)
brenda
Wadham, C.; Mangoni, A.A.
Dimethylarginine dimethylaminohydrolase regulation: a novel therapeutic target in cardiovascular disease
Expert. Opin. Drug Metab. Toxicol.
5
303-319
2009
Bos taurus, Bos taurus (Q3SX44), Oryctolagus cuniculus, Rattus norvegicus (O08557), Rattus norvegicus (Q6MG60), Homo sapiens (O94760), Homo sapiens (O95865), Mus musculus (Q99LD8), Mus musculus (Q9CWS0)
brenda
Pope, A.J.; Karrupiah, K.; Kearns, P.N.; Xia, Y.; Cardounel, A.J.
Role of dimethylarginine dimethylaminohydrolases in the regulation of endothelial nitric oxide production
J. Biol. Chem.
284
35338-35347
2009
Bos taurus (P56965), Bos taurus (Q3SX44)
brenda