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Information on EC 3.5.2.7 - imidazolonepropionase and Organism(s) Bacillus subtilis and UniProt Accession P42084

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Bacillus subtilis
UNIPROT: P42084 not found.
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
imidazolonepropionase, imidazolone-5-propionate hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Imidazolone-5-propionate hydrolase
-
imidazolonepropionase
-
4-imidazolone-5-propanoate amidohydrolase
-
-
-
-
Imidazolone-5-propionate hydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-91-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O
N-formimidoyl-L-glutamate + H+
show the reaction diagram
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
show the reaction diagram
-
-
-
?
imidazole-4-acetic acid + H2O
?
show the reaction diagram
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
show the reaction diagram
-
-
-
ir
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O
N-formimidoyl-L-glutamate + H+
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.12
-
streptomycin-treated extract of Bacillus subtilis, not purified
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
around pH 7.5
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
imidazolonepropionase (HutI) catalyzes the hydrolytic cleavage of carbon-nitrogen bond in 4-imidazolone-5-propionic acid (IPA) to yield L-formiminoglutamic acid, which is the third step in the universal histidine degradation pathway
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.0 A resolution
co-crystallization with imidazole-4-acetic acid sodium in 20% (w/v) polyethylene glycol 4000, 0.1 M Tris-HCl, pH 7.5, 0.2 M sodium acetate, and 2% (w/v) benzamidine hydrochloride
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Magasanik, B.; Kaminskas, E.; Kimhi, Y.
Imidazolonepropionic acid hydrolase (Bacillus subtilis)
Methods Enzymol.
17B
55-57
1971
Bacillus subtilis
-
Manually annotated by BRENDA team
Yu, Y.; Li, L.; Zheng, X.; Liang, Y.; Su, X.
Protein preparation, crystallization and preliminary x-ray analysis of imidazolonepropionase from Bacillus subtilis
Biochim. Biophys. Acta
1764
153-156
2006
Bacillus subtilis (P42084), Bacillus subtilis
Manually annotated by BRENDA team
Yu, Y.; Liang, Y.H.; Brostromer, E.; Quan, J.M.; Panjikar, S.; Dong, Y.H.; Su, X.D.
A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis
J. Biol. Chem.
281
36929-36936
2006
Bacillus subtilis (P42084), Bacillus subtilis
Manually annotated by BRENDA team
Yang, F.; Chu, W.; Yu, M.; Wang, Y.; Ma, S.; Dong, Y.; Wu, Z.
Local structure investigation of the active site of the imidazolonepropionase from Bacillus subtilis by XANES spectroscopy and ab initio calculations
J. Synchrotron Radiat.
15
129-133
2008
Bacillus subtilis (P42084), Bacillus subtilis
Manually annotated by BRENDA team
Su, H.; Sheng, X.; Liu, Y.
Exploring the substrate specificity and catalytic mechanism of imidazolonepropionase (HutI) from Bacillus subtilis
Phys. Chem. Chem. Phys.
18
27928-27938
2016
Bacillus subtilis (P42084), Bacillus subtilis, Bacillus subtilis 168 (P42084)
Manually annotated by BRENDA team