We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 3.5.2.7 - imidazolonepropionase for references in articles please use BRENDA:EC3.5.2.7Word Map on EC 3.5.2.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of peptide bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-histidine degradation I
-
-
L-histidine degradation II
-
-
L-histidine degradation III
-
-
L-histidine degradation VI
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate amidohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-imidazolone-5-propanoate amidohydrolase
-
-
-
-
imidazolone-5-propanoate hydrolase
-
Imidazolone-5-propionate hydrolase
HutI
-
Imidazolone-5-propionate hydrolase
-
-
-
-
Imidazolone-5-propionate hydrolase
-
-
Imidazolone-5-propionate hydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
grown on histidine
-
-
brenda
grown on histidine
-
-
brenda
-
-
-
brenda
grown on histidine
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4(5)-imidazolone-5(4)-propionic acid + H2O
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
imidazole-4-acetic acid + H2O
?
-
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
r
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
?
4(5)-imidazolone-5(4)-propionic acid + H2O
N-formimino-L-glutamate
-
-
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4(5)-imidazolone-5(4)-propionic acid + H2O
?
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
4(5)-imidazolone-5(4)-propionic acid + H2O
?
-
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Zn2+
-
contains zinc
Zn2+
-
contains a four-coordinated Zn2+ active site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-(2,5-dioxoimidazolidin-4-yl)propionic acid
-
-
p-chloromercuribenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
glutathione
-
reduced, partially reversed inhibition of the enzyme by p-chloromercuribenzoate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.1 - 1.54
4(5)-imidazolone-5(4)-propionic acid
0.1
4(5)-imidazolone-5(4)-propionic acid
-
-
0.2
4(5)-imidazolone-5(4)-propionic acid
-
-
1.54
4(5)-imidazolone-5(4)-propionic acid
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.12
-
streptomycin-treated extract of Bacillus subtilis, not purified
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.4
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240);
Agrobacterium fabrum (strain C58 / ATCC 33970);
Bacillus subtilis (strain 168);
A0KF84
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240);
A0KF84
Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240);
Q8U8Z6
Agrobacterium fabrum (strain C58 / ATCC 33970);
Q8U8Z6
Agrobacterium fabrum (strain C58 / ATCC 33970);
P42084
Bacillus subtilis (strain 168);
P42084
Bacillus subtilis (strain 168);
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homodimer
-
in the crystalline state, X-ray crystallography
homodimer
-
x-ray crystallography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
apoenzyme crystals for HutI are obtained by the sitting drop vapour diffusion method with 1.8 M triammonium citrate (pH 7.0) and 25% (w/v) PEG 3350, 0.1 M Bis-Tris (pH 5.5), and 0.2 M MgCl2, N-formimino-L-glutamate is cocrystallized with HutI via hanging drop vapor diffusion at 20°C against a reservoir solution containing 25% (w/v) PEG 3350, 0.1 M Bis-Tris (pH 5.5), and 0.2 M MgCl2
-
sitting drop vapour diffusion method with 25% (w/v) PEG 3350, 0.1M Bis-Tris pH 5.5, and 0.2 M MgCl2
-
co-crystallization with imidazole-4-acetic acid sodium in 20% (w/v) polyethylene glycol 4000, 0.1 M Tris-HCl, pH 7.5, 0.2 M sodium acetate, and 2% (w/v) benzamidine hydrochloride
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, 20 h, 50% loss of activity, stabilized by addition of MgCl2
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
expression in Escherichia coli
-
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Consevage, M.W.; Porter, R.D.; Phillips, A.T.
Cloning and expression in Escherichia coli of histidine utilization genes from Pseudomonas putida
J. Bacteriol.
162
138-146
1985
Pseudomonas putida
brenda
Kendrick, K.E.; Wheelis, M.L.
Histidine dissimilation in Streptomyces coelicolor
J. Gen. Microbiol.
128
2029-2040
1982
Streptomyces coelicolor
brenda
Snyder, S.H.
Imidazolonepropionic acid hydrolase (Rat liver)
Methods Enzymol.
17B
92-95
1971
Rattus norvegicus
-
brenda
Magasanik, B.; Kaminskas, E.; Kimhi, Y.
Imidazolonepropionic acid hydrolase (Bacillus subtilis)
Methods Enzymol.
17B
55-57
1971
Bacillus subtilis
-
brenda
Holm, L.; Sander, C.
An evolutionary treasure: Unification of a broad set of amidohydrolases related to urease
Proteins Struct. Funct. Genet.
28
72-82
1997
Caenorhabditis elegans
brenda
Smith, G.R.; Halpern, Y.S.; Magasanik, B.
Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase
J. Biol. Chem.
246
3320-3329
1971
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Snyder, S.H.; Silva, O.L.; Kies, M.W.
The mammalian metabolism of L-histidine: IV. Purification and properties of imidazolone propionic acid hydrolase
J. Biol. Chem.
236
2996-2998
1961
Rattus norvegicus
brenda
Rao, D.R.; Greenberg, D.M.
Studies on the enzymic decomposition of urocanic acid
J. Biol. Chem.
236
1758-1763
1961
Bos taurus, Cavia porcellus, Clostridium tetanomorphum, Klebsiella aerogenes, Ovis aries, Pseudomonas fluorescens, Rattus norvegicus, Sus scrofa
brenda
Yu, Y.; Li, L.; Zheng, X.; Liang, Y.; Su, X.
Protein preparation, crystallization and preliminary x-ray analysis of imidazolonepropionase from Bacillus subtilis
Biochim. Biophys. Acta
1764
153-156
2006
Bacillus subtilis, Bacillus subtilis (P42084)
brenda
Tyagi, R.; Eswaramoorthy, S.; Burley, S.K.; Raushel, F.M.; Swaminathan, S.
A common catalytic mechanism for proteins of the HutI family
Biochemistry
47
5608-5615
2008
Agrobacterium tumefaciens, Agrobacterium tumefaciens (Q8U8Z6)
brenda
Yu, Y.; Liang, Y.H.; Brostromer, E.; Quan, J.M.; Panjikar, S.; Dong, Y.H.; Su, X.D.
A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis
J. Biol. Chem.
281
36929-36936
2006
Bacillus subtilis, Bacillus subtilis (P42084)
brenda
Yang, F.; Chu, W.; Yu, M.; Wang, Y.; Ma, S.; Dong, Y.; Wu, Z.
Local structure investigation of the active site of the imidazolonepropionase from Bacillus subtilis by XANES spectroscopy and ab initio calculations
J. Synchrotron Radiat.
15
129-133
2008
Bacillus subtilis, Bacillus subtilis (P42084)
brenda
Tyagi, R.; Kumaran, D.; Burley, S.K.; Swaminathan, S.
X-ray structure of imidazolonepropionase from Agrobacterium tumefaciens at 1.87 A resolution
Proteins Struct. Funct. Bioinform.
69
652-658
2007
Agrobacterium tumefaciens, Agrobacterium tumefaciens (Q8U8Z6)
brenda
html completed