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Information on EC 3.5.2.6 - beta-lactamase and Organism(s) Pseudomonas fluorescens and UniProt Accession P85302

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.6 beta-lactamase
IUBMB Comments
A group of enzymes of varying specificity hydrolysing beta-lactams; some act more rapidly on penicillins, some more rapidly on cephalosporins. The latter were formerly listed as EC 3.5.2.8, cephalosporinase.
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This record set is specific for:
Pseudomonas fluorescens
UNIPROT: P85302
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas fluorescens
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-lactamase, carbapenemase, extended-spectrum beta-lactamase, ndm-1, penicillinase, tem-1, blandm-1, ges-1, blactx-m-15, kpc-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
class C beta-lactamase
-
Beta lactamase OXA-10
-
-
-
-
beta-lactamase
-
-
-
-
beta-lactamase AME I
-
-
-
-
beta-lactamase II
-
-
-
-
BLAIMP
-
-
-
-
carbapenemase
-
-
-
-
Carbenicillinase
-
-
-
-
cefotaximase
-
-
-
-
ceftazidimase
-
-
-
-
cefurooximase
-
-
-
-
Cefuroximase
-
-
-
-
Cephalosporinase
-
-
-
-
Imipenem-cefoxitin hydrolyzing enzyme
-
-
-
-
imipenemase
-
-
-
-
metallo-beta-lactamase
-
-
-
-
neutrapen
-
-
-
-
Oxacillinase
-
-
-
-
penicillinase
-
-
-
-
SHV-2A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-lactam hydrolase
A group of enzymes of varying specificity hydrolysing beta-lactams; some act more rapidly on penicillins, some more rapidly on cephalosporins. The latter were formerly listed as EC 3.5.2.8, cephalosporinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9073-60-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ampicillin + H2O
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
show the reaction diagram
-
-
-
?
benzylpenicillin + H2O
(2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
show the reaction diagram
-
-
-
?
carbenicillin + H2O
(2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
show the reaction diagram
-
-
-
?
cefazolin + H2O
(2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
show the reaction diagram
-
-
-
?
cephalexin + H2O
(2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
show the reaction diagram
-
-
-
?
cloxacillin + H2O
(2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
show the reaction diagram
-
-
-
?
nitrocefin + H2O
(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
show the reaction diagram
-
-
-
?
oxacillin + H2O
(2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0051
ampicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.0023
benzylpenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.0037
carbenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.15
cefazolin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.05
cephalexin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.00016
Cloxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.116
nitrocefin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.000118
Oxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
ampicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
34.3
benzylpenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.52
carbenicillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
61
cefazolin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
31
cephalexin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.42
Cloxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
550
nitrocefin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
0.21
Oxacillin
at 30°C in 50mM sodium phosphate buffer (pH 7.0)
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AMPC_PSEFL
358
0
38728
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38720
MALDI-TOF mass spectrometry
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method with 20% poly(ethylene)glycol 6 K in 0.1 M Tris (pH 8.0)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Michaux, C.; Massant, J.; Kerff, F.; Frere, J.M.; Docquier, J.D.; Vandenberghe, I.; Samyn, B.; Pierrard, A.; Feller, G.; Charlier, P.; Van Beeumen, J.; Wouters, J.
Crystal structure of a cold-adapted class C beta-lactamase
FEBS J.
275
1687-1697
2008
Psychrobacter immobilis, Serratia marcescens, Enterobacter cloacae (P05364), Pseudomonas fluorescens (P85302), Pseudomonas fluorescens, Pseudomonas fluorescens TAE4 (P85302), Enterobacter cloacae 908R (P05364)
Manually annotated by BRENDA team