We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
methylhydantoin amidase, N-methylhydantoin amidohydohydrolase, N-methylhydantoin amidohydrolase, N-methylhydantoin hydrolase, N-methylhydantoinase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methylhydantoin amidase
-
-
-
-
N-methylhydantoin amidohydohydrolase
-
-
-
-
N-methylhydantoin amidohydrolase
A0A222FYV4; A0A222FYQ1
-
N-methylhydantoin hydrolase
-
-
-
-
N-methylhydantoinase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + N-methylhydantoin + 2 H2O = ADP + phosphate + N-carbamoylsarcosine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of amide bond
-
-
-
-
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N-methylimidazolidine-2,4-dione amidohydrolase (ATP-hydrolysing)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + 2,4-thiazolidinedione + H2O
?
-
-
-
-
?
ATP + 2-imidazolidone + H2O
?
-
-
-
-
?
ATP + 2-oxazolidone + H2O
?
-
-
-
-
?
ATP + 2-pyrrolidone + H2O
?
-
-
-
-
?
ATP + D,L-5-methylhydantoin + H2O
?
-
-
-
-
?
ATP + delta-valerolactam + H2O
?
-
-
-
-
?
ATP + dihydrothymine + H2O
?
-
-
-
-
?
ATP + dihydrouracil + H2O
?
-
-
-
-
?
ATP + glutarimide + H2O
?
-
-
-
-
?
ATP + hydantoin + H2O
?
-
-
-
-
?
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
ATP + succimide + H2O
?
-
-
-
-
?
ATP + thymine + H2O
?
-
-
-
-
?
ATP + uracil + H2O
?
-
-
-
-
?
CTP + dihydrouracil + H2O
?
-
-
-
-
?
dATP + dihydrouracil + H2O
?
-
-
-
-
?
dATP + N-methylimidazolidine-2,4-dione + H2O
dADP + phosphate + N-carbamoylsarcosine
-
-
-
-
?
dGTP + dihydrouracil + H2O
?
-
-
-
-
?
dITP + dihydrouracil + H2O
?
-
-
-
-
?
dUTP + dihydrouracil + H2O
?
-
-
-
-
?
GTP + dihydrouracil + H2O
?
-
-
-
-
?
ITP + dihydrouracil + H2O
?
-
-
-
-
?
N-methylpyrrolidone +
gamma-N-methylaminobutyric acid
A0A222FYV4; A0A222FYQ1
-
-
-
?
UTP + dihydrouracil + H2O
?
-
-
-
-
?
XTP + dihydrouracil + H2O
?
-
-
-
-
?
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
-
-
-
-
?
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
-
-
-
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
-
-
-
-
?
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
-
-
-
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
K+
-
-
Mg2+
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,5'-dithio-bis(2-nitrobenzoic acid)
-
0.1 mM
8-hydroxyquinoline
-
1 mM
N-methylhydantoin
-
inhibits the hydrolysis of other nucleoside triphosphates than ATP and dATP
p-chloromercuribenzoate
-
0.1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10
2,4-Thiazolidinedione
-
-
2
D,L-5-methylhydantoin
-
-
0.11
delta-valerolactam
-
-
0.032
N-methylhydantoin
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45
-
ATP in presence of N-methylhydantoin
50
-
CTP in absence of N-methylhydantoin
55
-
CTP in presence of N-methylhydantoin
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A0A222FYV4: N-methylhydantoin amidohydrolase alpha-subunit. A0A222FYQ1: N-methylhydantoin amidohydrolase beta-subunit
A0A222FYV4; A0A222FYQ1
UniProt
brenda
77
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
A0A222FYV4; A0A222FYQ1
the enzyme is involved in the biodegradation of N-methylpyrrolidone, a widely used industrial solvent that produces skin irritation in humans and is teratogenic in rats
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
70000
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
80000
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
tetramer
-
alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
addition of small portions of solid ammonium sulfate to the concentrated enzyme solution
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10.6
-
loss of 30% in activity
246618
5
-
loss of 25% in activity
246618
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
60
-
about 70% of initial activity remains
70
-
about 20% of initial activity remains
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
analysis
-
measurement of serum and urine creatinine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ogawa, J.; Min Kim, J.; Nirdnoy, W.; Amano, Y.; Yamada, H.; Shimizu, S.
Purification and characterization of an ATP-dependent amidohydrolase, N-methylhydantoin amidohydrolase, from Pseudomonas putida 77
Eur. J. Biochem.
229
184-190
1995
Pseudomonas putida
-
brenda
Ogawa, J.; Warawadee, N.; Masayoshi, T.; Hideaki, Y.; Sakayu, S.
A new enzymatic method for measurement of creatinine involving a novel ATP-dependent enzyme, N-methylhydantoin amidohydrolase
Biosci. Biotechnol. Biochem.
59
2292-2294
1995
Pseudomonas putida
brenda
Ogawa, J.; Warawadee, N.; Hideaki, Y.; Sakayu, S.
Nucleoside triphosphatase activity of an ATP-dependent enzyme, N-methylhydantoin amidohydrolase
Biosci. Biotechnol. Biochem.
59
1737-1739
1995
Pseudomonas putida
-
brenda
Min Kim, J.; Shimizu, S.; Yamada, H.
Amidohydrolysis of N-methylhydantoin coupled with ATP hydrolysis
Biochem. Biophys. Res. Commun.
142
1006-1012
1987
Pseudomonas putida
brenda
Solís-González, C.; Domínguez-Malfavón, L.; Vargas-Suárez, M.; Gaytán, I.; Cevallos, M.; Lozano, L.; Cruz-Gómez, M.; Loza-Tavera, H.
Novel metabolic pathway for N-methylpyrrolidone degradation in Alicycliphilus sp. strain BQ1
Appl. Environ. Microbiol.
84
e02136-17
2018
Alicycliphilus denitrificans (A0A222FYV4 AND A0A222FYQ1)
-
brenda
Select items on the left to see more content.
html completed