Information on EC 3.5.2.14 - N-methylhydantoinase (ATP-hydrolysing)
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EC Tree
The expected taxonomic range for this enzyme is: Pseudomonas putida
Synonyms
n-methylhydantoinase,
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methylhydantoin amidase
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N-methylhydantoin amidohydohydrolase
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N-methylhydantoin hydrolase
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N-methylhydantoinase
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ATP + N-methylhydantoin + 2 H2O = ADP + phosphate + N-carbamoylsarcosine
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hydrolysis of amide bond
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hydrolysis of phosphoric ester
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N-methylimidazolidine-2,4-dione amidohydrolase (ATP-hydrolysing)
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ATP + 2,4-thiazolidinedione + H2O
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?
ATP + 2-imidazolidone + H2O
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?
ATP + 2-oxazolidone + H2O
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?
ATP + 2-pyrrolidone + H2O
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?
ATP + D,L-5-methylhydantoin + H2O
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?
ATP + delta-valerolactam + H2O
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?
ATP + dihydrothymine + H2O
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?
ATP + dihydrouracil + H2O
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ATP + glutarimide + H2O
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?
ATP + hydantoin + H2O
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ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
ATP + succimide + H2O
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?
ATP + thymine + H2O
?
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?
ATP + uracil + H2O
?
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?
CTP + dihydrouracil + H2O
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?
dATP + dihydrouracil + H2O
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?
dATP + N-methylimidazolidine-2,4-dione + H2O
dADP + phosphate + N-carbamoylsarcosine
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?
dGTP + dihydrouracil + H2O
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?
dITP + dihydrouracil + H2O
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?
dUTP + dihydrouracil + H2O
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?
GTP + dihydrouracil + H2O
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ITP + dihydrouracil + H2O
?
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?
UTP + dihydrouracil + H2O
?
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?
XTP + dihydrouracil + H2O
?
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?
ATP + dihydrouracil + H2O
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ATP + dihydrouracil + H2O
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ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
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ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
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ir
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ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
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ATP + N-methylimidazolidine-2,4-dione + H2O
ADP + phosphate + N-carbamoylsarcosine
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ir
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K+
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monovalent cations
Mg2+
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divalent cations
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5,5'-dithio-bis(2-nitrobenzoic acid)
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0.1 mM
8-hydroxyquinoline
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1 mM
N-methylhydantoin
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inhibits the hydrolysis of other nucleoside triphosphates than ATP and dATP
p-chloromercuribenzoate
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0.1 mM
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10
2,4-Thiazolidinedione
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2
D,L-5-methylhydantoin
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0.11
delta-valerolactam
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0.032
N-methylhydantoin
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45
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ATP in presence of N-methylhydantoin
50
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CTP in absence of N-methylhydantoin
55
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CTP in presence of N-methylhydantoin
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77
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brenda
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brenda
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70000
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alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
80000
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alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
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tetramer
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alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE
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addition of small portions of solid ammonium sulfate to the concentrated enzyme solution
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5
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loss of 25% in activity
246618
10.6
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loss of 30% in activity
246618
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60
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about 70% of initial activity remains
70
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about 20% of initial activity remains
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analysis
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measurement of serum and urine creatinine
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Ogawa, J.; Min Kim, J.; Nirdnoy, W.; Amano, Y.; Yamada, H.; Shimizu, S.
Purification and characterization of an ATP-dependent amidohydrolase, N-methylhydantoin amidohydrolase, from Pseudomonas putida 77
Eur. J. Biochem.
229
184-190
1995
Pseudomonas putida
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brenda
Ogawa, J.; Warawadee, N.; Masayoshi, T.; Hideaki, Y.; Sakayu, S.
A new enzymatic method for measurement of creatinine involving a novel ATP-dependent enzyme, N-methylhydantoin amidohydrolase
Biosci. Biotechnol. Biochem.
59
2292-2294
1995
Pseudomonas putida
brenda
Ogawa, J.; Warawadee, N.; Hideaki, Y.; Sakayu, S.
Nucleoside triphosphatase activity of an ATP-dependent enzyme, N-methylhydantoin amidohydrolase
Biosci. Biotechnol. Biochem.
59
1737-1739
1995
Pseudomonas putida
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brenda
Min Kim, J.; Shimizu, S.; Yamada, H.
Amidohydrolysis of N-methylhydantoin coupled with ATP hydrolysis
Biochem. Biophys. Res. Commun.
142
1006-1012
1987
Pseudomonas putida
brenda
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3.5.2.14 N-methylhydantoinase (ATP-hydrolysing)
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