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IUBMB Comments Identified in Pseudomonas putida . Strict specificity for N- acetyl arylalkylamines, including N- acetyl-2-phenylethylamine, N- acetyl-3-phenylpropylamine, N- acetyldopamine, N- acetyl-serotonin and melatonin. It also accepts arylalkyl acetates but not acetanilide derivatives, which are common substrates of EC 3.5.1.13 , aryl acylamidase.
Synonyms
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Arylalkyl acylamidase
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N-Acetylarylalkylamine + H2O = arylalkylamine + acetate
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hydrolysis of linear amides
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N-Acetylarylalkylamine amidohydrolase
Identified in Pseudomonas putida. Strict specificity for N-acetyl arylalkylamines, including N-acetyl-2-phenylethylamine, N-acetyl-3-phenylpropylamine, N-acetyldopamine, N-acetyl-serotonin and melatonin. It also accepts arylalkyl acetates but not acetanilide derivatives, which are common substrates of EC 3.5.1.13, aryl acylamidase.
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(R)-N-acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-methyl-3-phenylpropylamine + acetate
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Substrates: -
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Acetic acid 2-phenylethyl ester + H2O
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Substrates: -
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Acetic acid 3-phenylpropyl ester + H2O
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Substrates: -
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Melatonin + H2O
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Substrates: -
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N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
N-Acetyl-2-phenylethylamine + H2O
2-Phenylethylamine + acetate
N-Acetyl-3-phenylpropylamine
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N-Acetylbenzylamine + H2O
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N-acetyldopamine + H2O
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Substrates: -
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N-Acetylserotonin + H2O
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Substrates: -
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additional information
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N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
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Substrates: -
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N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
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Substrates: (R)-isomer
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N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
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Substrates: (R)-isomer
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N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
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Substrates: -
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N-Acetyl-2-phenylethylamine + H2O
2-Phenylethylamine + acetate
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Substrates: -
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N-Acetyl-2-phenylethylamine + H2O
2-Phenylethylamine + acetate
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Substrates: -
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N-Acetyl-3-phenylpropylamine
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Substrates: -
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N-Acetyl-3-phenylpropylamine
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Substrates: -
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N-Acetylbenzylamine + H2O
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Substrates: -
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N-Acetylbenzylamine + H2O
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Substrates: -
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additional information
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Substrates: no activity with acetanilide derivatives
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additional information
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Substrates: no activity with acetanilide derivatives
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5,5'-dithiobis(2-nitrobenzoic acid)
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weak
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8.9
(R)-N-acetyl-1-methyl-3-phenylpropylamine
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0.31
N-acetyl-2-phenylethylamine
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1.6
N-Acetyl-3-phenylpropylamine
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41
N-Acetylbenzylamine
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0.34
N-acetylserotonin
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8
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with N-acetyl-2-phenylethylamine as substrate
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6 - 10
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pH 6.0: about 50% of maximal activity, pH 10: about 40% of maximal activity, with N-acetyl-2-phenylethalamine as substrate
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55 - 75
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55°C: about 45% of maximal activity, 75°C: about 20% of maximal activity
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Highest Expressing Human Cell Lines
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ARYA_PSEPU
28
0
3150
Swiss-Prot
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42000
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4 * 42000, SDS-PAGE
42630
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4 * 42630, SDS-PAGE
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tetramer
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4 * 42000, SDS-PAGE
tetramer
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4 * 42630, SDS-PAGE
tetramer
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4 * 42000, SDS-PAGE
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tetramer
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4 * 42630, SDS-PAGE
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7
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30°C, 30 min, most stable under alkaline conditions
31889
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60
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pH 7.0, stable below
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Shimizu, S.; Ogawa, J.; Chung, M.C.M.; Yamada, H.
Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2
Eur. J. Biochem.
209
375-382
1992
Pseudomonas putida, Pseudomonas putida Sc2
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Ogawa, J.; Shimizu, S.; Yamada, H.
Enzymatic asymetric synthesis of alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols by arylalkyl acylamidases
Bioorg. Med. Chem.
2
429-432
1994
Pseudomonas putida, Pseudomonas putida Sc2
brenda
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