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Information on EC 3.5.1.72 - D-benzoylarginine-4-nitroanilide amidase
for references in articles please use BRENDA:EC3.5.1.72
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EC Tree 3 Hydrolases
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.5.1 In linear amides
3.5.1.72 D-benzoylarginine-4-nitroanilide amidase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
d-bapaase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
amidase, benzoyl-D-arginine aryl-
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-
-
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benzoyl-D-arginine arylamidase
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-
-
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D-BAPA-ase
-
-
-
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D-BAPA-hydrolyzing enzyme
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-
-
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D-BAPAase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-benzoyl-D-arginine-4-nitroanilide + H2O = N-benzoyl-D-arginine + 4-nitroaniline
stereospecific enzyme activity to D-isomer
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
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-
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-
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SYSTEMATIC NAME
IUBMB Comments
N-benzoyl-D-arginine-4-nitroanilide amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
119345-26-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
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Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
marine aerobic sporeforming bacterium 6A10
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Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
N-benzoyl-D-Arg-4-nitroanilide + H2O
N-benzoyl-D-Arg + 4-nitroaniline
-
Substrates: D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
additional information
?
-
-
Substrates: ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CaCl2
MgCl2
NaCl
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
N-benzoyl-D-Arg-4-nitroanilide
-
substrate inhibition at concentration above 1 mM
N-benzoyl-D-Arg-4-nitroanilide
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substrate inhibition at concentration above 0.5 mM
additional information
-
no inhibition by EDTA; strain 168, no inhibition by lysozyme, 2 mg/ml, or cell wall lysate
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
-
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
brenda
additional information
-
enzyme is more active in vegetative cells than in germinating spores
-
brenda
additional information
marine aerobic sporeforming bacterium 6A10
-
enzyme is more active in vegetative cells than in germinating spores
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
strain 168, subcellular distribution, no activity in cell wall lysate and membranes
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
66000
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
-
strain 168, 2 min, 40% loss of activity in 0.05 M Tris buffer, pH 7.8, 60% loss of activity with 5 mM MgCl2, 80% loss of activity with 5 mM CaCl2
additional information
-
strain 168, Ca2+ and Mg2+ ions decrease thermostability
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gofshtein-Gandman, L.V.; Keynan, A.; Milner, Y.
Bacteria of the genus Bacillus have a hydrolase stereospecific to the D isomer of benzoyl-arginine-p-nitroanilide
J. Bacteriol.
170
5895-5900
1988
Bacillaceae, Bacillus subtilis, Bacillus cereus, Lysinibacillus sphaericus, Bacillus thuringiensis, Clostridium sp., marine aerobic sporeforming bacterium 6A10, no activity in Streptococcus mutans serotype C, no activity in Streptococcus pneumoniae, no activity in Corynebacterium bovis, no activity in Escherichia coli, no activity in Acetobacter xylinum, no activity in Escherichia coli JM83, Bacillus cereus T, Lysinibacillus sphaericus 2362
brenda
Oren, A.; Gofshtein-Gandman, L.V.; Keynan, A.
Hydrolysis of N'-benzoyl-D-arginine-p-nitroanilide by members of the Haloanaerobiaceae: additional evidence that Haloanaerobium praevalens is related to endospore-forming bacteria
FEMS Microbiol. Lett.
58
5-10
1989
Bacillus cereus, Clostridium sp., Halanaerobium praevalens, Halobacteroides halobius, Orenia marismortui, no activity in Sporohalobacter lortetii, no activity in Vibrio costicola, Bacillus cereus T
-
brenda
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