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Enzyme Nomenclature
Information on EC 3.5.1.66 - 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
3.5.1.66
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RECOMMENDED NAME
GeneOntology No.
2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + 2 H2O = acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
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deaminating, decarboxylating
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
2-(hydroxymethyl)-3-(acetamidomethylene)succinate amidohydrolase (deaminating, decarboxylating)
Involved in the degradation of pyridoxin by Pseudomonas and Arthrobacter.
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CAS REGISTRY NUMBER
COMMENTARY
95829-26-8
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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involved in pyridoxine degradation
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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?
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
acetate + 2-(hydroxymethyl)-4-oxobutanoate + NH3 + CO2
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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?
3-(acetamidomethylene)succinate + H2O
acetate + 4-oxobutanoate + NH3 + CO2
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compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
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?
3-(acetamidomethylene)succinate + H2O
acetate + 4-oxobutanoate + NH3 + CO2
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compound A, alpha-(N-acetylaminomethylene)succinic acid: poor substrate
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?
additional information
?
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enzyme highly specific, inducible amidohydrolase
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additional information
?
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enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
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additional information
?
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enzyme highly specific, inducible amidohydrolase
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additional information
?
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enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
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additional information
?
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enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
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additional information
?
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enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
-
compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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involved in pyridoxine degradation
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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2-(hydroxymethyl)-3-(acetamidomethylene)succinate + H2O
?
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compound B, alpha-hydroxymethyl-alpha'-(N-acetylaminomethylene)succinic acid
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additional information
?
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enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
-
-
-
additional information
?
-
-
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
-
-
-
additional information
?
-
-
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
-
-
-
additional information
?
-
-
enzyme of vitamin B6 degradation, utilizing pyridoxine as sole source of carbon and nitrogen
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
sulfite
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noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
additional information
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no inhibition by: organic protein reagents, 0.1 mM, 15 min, p-chloromercuribenzoate, 1.0 mM Ni2+, 1.0 mM Co2+, 1.0 mM Mn2+, 1.0 mM Mg2+, 1.0 mM Ca2+, 1.0 mM Cu2+, 1.0 mM Cs+, 1.0 mM Li+, SO42-, NO3-, Cl-, 10 mM dithiothreitol, 100 mM monocarboxylic acids
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.025
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
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compound B, 25°C, Tris-HCl, pH 7.0
0.123
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
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compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.0
0.25
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
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compound B, Tris-HCl, pH 8.0
0.56
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
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compound B, 2-(N-morpholino)ethane-sulfonic acid, pH 6.5
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.3
2-(Hydroxymethyl)-3-(acetamidomethylene)succinate
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turnover no./subunit, 25°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
multimer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.5.1.66)
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