Information on EC 3.5.1.55 - long-chain-fatty-acyl-glutamate deacylase

for references in articles please use BRENDA:EC3.5.1.55
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

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EC NUMBER
COMMENTARY hide
3.5.1.55
-
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RECOMMENDED NAME
GeneOntology No.
long-chain-fatty-acyl-glutamate deacylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-long-chain-fatty-acyl-L-glutamate + H2O = a long-chain carboxylate + L-glutamate
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
N-long-chain-fatty-acyl-L-glutamate amidohydrolase
Does not act on acyl derivates of other amino acids. Optimum chain length of acyl residue is 12 to 16.
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CAS REGISTRY NUMBER
COMMENTARY hide
82249-69-2
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-arachidonoyl-L-glutamate + H2O
arachidonate + L-glutamate
show the reaction diagram
-
32% of the activity with N-decanoyl-L-glutamate
-
?
N-benzoyl-L-glutamate + H2O
benzoate + L-glutamate
show the reaction diagram
-
74% of the activity with N-decanoyl-L-glutamate
-
?
N-decanoyl-L-glutamate + H2O
decanoate + L-glutamate
show the reaction diagram
N-lauroyl-L-aspartate + H2O
lauroate + L-aspartate
show the reaction diagram
N-lauroyl-L-glutamate + H2O
lauroate + L-glutamate
show the reaction diagram
N-lauroylglycine + H2O
lauroate + glycine
show the reaction diagram
-
17.5% of the activity with palmitoyl-L-glutamate
-
?
N-myristoyl-L-glutamate + H2O
myristoate + L-glutamate
show the reaction diagram
N-octanoyl-L-glutamate + H2O
octanoate + L-glutamate
show the reaction diagram
-
80% of the activity with decanoyl-L-glutamate
-
?
N-oleoyl-L-glutamate + H2O
oleate + L-glutamate
show the reaction diagram
-
67% of the activity with N-decanoyl-L-glutamate
-
?
N-palmitoyl-L-glutamate + H2O
palmitate + L-glutamate
show the reaction diagram
N-pentadecanoyl-L-glutamate + H2O
pentadecanoate + L-glutamate
show the reaction diagram
-
best substrate
-
?
N-stearoyl-L-glutamate + H2O
stearate + L-glutamate
show the reaction diagram
N-undecanoyl-L-glutamate + H2O
undecanoate + L-glutamate
show the reaction diagram
additional information
?
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates
Cd2+
-
activates
Cu2+
-
activates
Mg2+
-
activates
Ni2+
-
activates
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ba2+
-
slight inhibition
Cu2+
-
slight inhibition
iodoacetamide
-
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
-
reduced glutathione
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
N-lauroyl-L-aspartate
-
-
0.17 - 7.1
N-lauroyl-L-glutamate
2
N-lauroylglycine
-
-
0.083 - 5
N-myristoyl-L-glutamate
0.21
N-oleolyl-L-glutamate
-
-
0.21
N-oleoyl-L-glutamate
-
-
0.14 - 4.9
N-palmitoyl-L-glutamate
0.33 - 2
N-stearoyl-L-glutamate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
substrate lauroyl-DL-valine, lauroylglycine
9
-
substrate N-tearoyl-L-glutamate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
8 * 40000, enzyme I, SDS -PAGE
55000
-
4 * 55000, enzyme II, SDS-PAGE
192000
-
gel filtration
220000
-
enzyme II, gel filtration
300000
-
enzyme I, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
8 * 40000, enzyme I, SDS -PAGE
tetramer
-
4 * 55000, enzyme II, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
4°C, 24 h, stable, enzyme form II
209198
6
-
4°C, 24 h, 60% loss of activity, enzyme form I
209197
6 - 8
-
30°C, 5 h, stable
209199
7 - 11
-
4°C, 24 h, stable, enzyme form I
209197
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
pH 7, 10 min, almost complete loss of activity, enzyme II
60
-
pH 7, 10 min, quite stable, enzyme form I
70
-
pH 7, 10 min, complete inactivation, enzyme form I
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 3.5.1.55)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)