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Enzyme Nomenclature
Information on EC 3.5.1.24 - choloylglycine hydrolase
for references in articles please use BRENDA:EC3.5.1.24
Word Map on EC 3.5.1.24
- 3.5.1.24
- lactobacillus
-
deconjugation
- plantarum
- taurine
-
cholic
- bifidobacterium
-
taurodeoxycholic
-
glycocholic
-
taurocholic
-
cholesterol-lowering
-
glycodeoxycholic
- acidophilus
-
oxgall
-
taurine-conjugated
- pentosus
- animalis
-
glycine-conjugated
- fermentum
-
auto-aggregation
- drug development
- medicine
- food industry
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Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
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EC NUMBER 
COMMENTARY 
3.5.1.24
-
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RECOMMENDED NAME
GeneOntology No.
choloylglycine hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glycocholate + H2O = cholate + glycine
-
-
-
-
glycocholate + H2O = cholate + glycine
substrate binding pocket and active site structure, taurine has a reversed orientation pointing with its sulfo group towards Cys2 and leaving the active site with its amino group ahead, overview
-
glycocholate + H2O = cholate + glycine
substrate specificity and active site structure, residues Cys1, Arg17, Asp20, Asn174, and Arg227 are important for catalysis, Trp21 plays a selective role in binding of bile salt while it suppresses productive binding of substrate analogue penicillin V
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
hydrolysis
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine amidohydrolase
Also acts on the 3alpha,12alpha-dihydroxy-derivative, and on choloyl-taurine.
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CAS REGISTRY NUMBER
COMMENTARY
37289-07-9
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subps. animalis , isolated from rat feces
UniProt
subsp. pseudolongum, isolated from bovine rumen
UniProt
human intestinal strain 016, human intestinal strain L1, porcine intestinal strain ATCC 43121
-
-
BSH C; formerly Lactobacillus acidophilus strain PF01, gene PF01_00682, isozyme BSH C
F7SHH3
UniProt
isozyme BSH A; formerly Lactobacillus acidophilus strain PF01, gene PF01_00515, isozyme BSH A
F7SGU2
UniProt
isozyme BSH B; formerly Lactobacillus acidophilus strain PF01, gene PF01_01578, isozyme BSH B
F7SGA1
UniProt
strain 100-100, two different antigenic conjugated BSHs, BSHalpha and BSHbeta
-
-
BSH C; formerly Lactobacillus acidophilus strain PF01, gene PF01_00682, isozyme BSH C
F7SHH3
UniProt
isozyme BSH A; formerly Lactobacillus acidophilus strain PF01, gene PF01_00515, isozyme BSH A
F7SGU2
UniProt
isozyme BSH B; formerly Lactobacillus acidophilus strain PF01, gene PF01_01578, isozyme BSH B
F7SGA1
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
physiological function
additional information
evolution
-
the BSH from Bifidobactrium longum demonstrates a evolutionary relationship with penicillin V acylase
evolution
comparative genomic, structural and biochemical analysis of the enzyme from Lactobacillus acidophilus and Lactobacillus salivarius, overview. The enzymes share similar structure by showing the typical canonic alphabetabetaalpha-folding pattern. The critical amino acids are also superimposed very well, particularly with respect to the typical Cys2, which serves as an N-terminal nucleophile, and Arg16, which play a potentially essential role in catalytic functioning of the enzyme
evolution
-
comparative genomic, structural and biochemical analysis of the enzyme from Lactobacillus acidophilus and Lactobacillus salivarius, overview. The enzymes share similar structure by showing the typical canonic alphabetabetaalpha-folding pattern. The critical amino acids are also superimposed very well, particularly with respect to the typical Cys2, which serves as an N-terminal nucleophile, and Arg16, which play a potentially essential role in catalytic functioning of the enzyme
evolution
phylogenetic tree; phylogenetic tree; phylogenetic tree
evolution
-
comparative genomic, structural and biochemical analysis of the enzyme from Lactobacillus acidophilus and Lactobacillus salivarius, overview. The enzymes share similar structure by showing the typical canonic alphabetabetaalpha-folding pattern. The critical amino acids are also superimposed very well, particularly with respect to the typical Cys2, which serves as an N-terminal nucleophile, and Arg16, which play a potentially essential role in catalytic functioning of the enzyme
-
evolution
-
comparative genomic, structural and biochemical analysis of the enzyme from Lactobacillus acidophilus and Lactobacillus salivarius, overview. The enzymes share similar structure by showing the typical canonic alphabetabetaalpha-folding pattern. The critical amino acids are also superimposed very well, particularly with respect to the typical Cys2, which serves as an N-terminal nucleophile, and Arg16, which play a potentially essential role in catalytic functioning of the enzyme
-
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
physiological function
-
BSH is an enzyme produced by several bacterial species in the human or animal gastrointestinal tract that catalyzes the glycine- or taurine-linked bile salt deconjugation reaction. Presence of bile salt hydrolase in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol level of the host, physiological functions of probiotics, overview
additional information
-
effect of oral administration to Wistar rats of the immobilized bile salt hydrolase enzyme on serum cholesterol, triglyceride, high density lipoprotein levels and its application in the therapeutic treatment of hypercholesteremia, overview
additional information
-
probiotic organisms colonizing the host gastrointestinal tract need to be bile salt tolerant
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,12-dioxo-5beta-cholanoylglycine + H2O
3,12-dioxo-5beta-cholanate + Gly
-
-
-
-
?
3,12-dioxo-5beta-cholanoyltaurine + H2O
3,12-dioxo-5beta-cholanate + taurine
-
-
-
-
?
3,7-dioxo-5beta-cholanoylglycine + H2O
3,7-dioxo-5beta-cholanate + Gly
-
-
-
-
?
3,7-dioxo-5beta-cholanoyltaurine + H2O
3,7-dioxo-5beta-cholanate + taurine
-
-
-
-
?
3-oxo-5beta-cholanoylglycine + H2O
3-oxo-5beta-cholanate + Gly
-
-
-
-
?
3-oxo-5beta-cholanoyltaurine + H2O
3-oxo-5beta-cholanate + taurine
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oylglycine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + glycine
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholan-24-oyltaurine + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-homocholanate + taurine
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-alpha-aminomethanesulfonic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + aminomethanesulfonic acid
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-beta-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + beta-Ala
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoyl-DL-Ala + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + DL-Ala
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholanoylsarcosine + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + sarcosine
-
low activity
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + Gly
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-cholestan-26-oyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-cholestanate + taurine
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoylglycine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + Gly
-
-
-
-
?
3alpha,7alpha-dihydroxy-5beta-homocholanoyltaurine + H2O
3alpha,7alpha-dihydroxy-5beta-homocholanate + taurine
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3alpha,7beta-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoylglycine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + Gly
-
-
-
-
?
3alpha-hydroxy-12-oxo-5beta-cholanoyltaurine + H2O
3alpha-hydroxy-12-oxo-5beta-cholanate + taurine
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanylglycine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + Gly
-
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholanyltaurine + H2O
3alpha-hydroxy-7-oxo-5beta-cholanate + taurine
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
3beta,7alpha-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoylglycine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
3beta,7beta-dihydroxy-5beta-cholanoyltaurine + H2O
3beta,7beta-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
cholyl-alpha-aminomethanesulfonic acid + H2O
cholic acid + aminomethanesulfonic acid
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycodeoxycholate + H2O
3alpha,12alpha-dihydroxy-5-beta-cholanate + glycine
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,12alphadihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
taurine + deoxycholate
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
-
-
-
-
?
glycochenodeoxycholate + H2O
glycine + chenodeoxycholate
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
J7H3P9
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
J7H3P9
best substrate
-
-
?
glycocholate + H2O
cholate + glycine
preferred substrate
-
-
?
glycocholate + H2O
cholate + glycine
preferred substrate
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
activity assay
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + Gly
-
activity assay
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
strain CK102
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
-
-
-
-
?
glycodeoxycholate + H2O
glycine + deoxycholate
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + Gly
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
I3QNS9
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
about 80% activity compared to glycocholic acid
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
isoform BSH1 shows much higher hydrolysis on glycodeoxycholic acid than isoforms BSH2, BSH3 and BSH4
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
J7H3P9
-
-
-
?
glycohyodeoxycholate + H2O
glycine + hyodeoxycholate
-
-
-
-
?
glycoursodeoxycholate + H2O
glycine + ursodeoxycholate
-
-
-
-
?
glycoursodeoxycholate + H2O
glycine + ursodeoxycholate
-
-
-
-
?
taurochenodeoxycholate + H2O
chenodeoxycholate + taurin
-
-
-
?
taurochenodeoxycholate + H2O
chenodeoxycholate + taurin
-
-
-
?
taurochenodeoxycholate + H2O
chenodeoxycholate + taurin
-
-
-
?
taurochenodeoxycholate + H2O
chenodeoxycholate + taurin
-
-
-
?
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
-
-
-
-
?
taurochenodeoxycholate + H2O
taurine + chenodeoxycholate
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
3alpha,7alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
J7H3P9
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
J7H3P9
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
hydrolyzing activity against tauroconjugated bile salts, but not glycoconjugated bile salts
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
-
-
-
-
?
taurodeoxycholate + H2O
taurine + deoxycholate
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
I3QNS9
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
less than 20% activity compared to glycocholic acid
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
J7H3P9
-
-
-
?
additional information
?
-
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
-
the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts
-
-
-
additional information
?
-
-
substrate specificity profile, overview
-
-
-
additional information
?
-
-
BSH can hydrolyze all the six major human bile salts and at least two animal bile salts. BSH from five strains show a better deconjugation rate on glycine-conjugated bile salts than on taurine-conjugated forms
-
-
-
additional information
?
-
-
the SH group in the N-terminal cysteine is responsible for BSH activity, and amino acids, viz. Asp20, Tyr82, Asn175, and Arg228, are believed to take part actively along with Cys in catalysis of bile salts
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
-
substrate specificity, overview
-
-
-
additional information
?
-
-
substrate specificity, overview
-
-
-
additional information
?
-
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
substrate specificity of the purified enzyme, overview
-
-
-
additional information
?
-
-
catalyzes the hydrolysis of glycine- and taurine-conjugated bile salts to amino acid residues and free bile salts
-
-
-
additional information
?
-
-
the enzyme hydrolyzes all of the six major human bile salts
-
-
-
additional information
?
-
-
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
-
BSH catalyzes the hydrolysis of amide bond in conjugated bile salts and free amino acids are released, which form the deconjugated bile acid, mainly cholic and quenodeoxycholic
-
-
-
additional information
?
-
-
BSHs is highly substrate-specific, the two isozymes, encoded by two bsh genes, show different substrate specificities. BSHs can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group. The recombinant BSH from strain PF01 is active with tauroconjugated bile salts, but not with glycoconjugated bile slats
-
-
-
additional information
?
-
-
strain JCM1069 exhibits hydrolase activity against the taurodeoxycholic acid but not against the taurocholic acid, although both acids have taurine as their amino acid moiety but vary in their steroid moieties at 7alpha position
-
-
-
additional information
?
-
-
BSHs is highly substrate-specific and can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group. No activity with taurocholic acid
-
-
-
additional information
?
-
the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates
-
-
-
additional information
?
-
the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates
-
-
-
additional information
?
-
-
the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates
-
-
-
additional information
?
-
F7SGA1
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
F7SGU2
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
F7SHH3
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
F7SGA1
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
F7SGU2
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
F7SHH3
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
-
isoyzme BSH A hydrolyses tauro-conjugated bile salts, whereas isozyme BSH C hydrolyses glycoconjugated bile salts. The enzymes have no preferential activities towards a specific cholyl moiety, the isozymes differ in their substrate specificities, overview
-
-
-
additional information
?
-
-
BSHs BSHs is highly substrate-specific and can identify its substrate, bile acids, on amino acid groups, glycine/taurine, and also on cholate steroid nucleus. BSH recognizes the cholate group
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
-
the recombinant enzyme is more efficient in hydrolyzing glycoconjugated bile salts than tauroconjugated bile salts
-
-
-
additional information
?
-
-
the recombinant enzyme is more efficient in hydrolyzing glycoconjugated bile salts than tauroconjugated bile salts
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
-
the Lactobacillus salivarius enzyme displays potent hydrolysis activity towards both glycoconjugated and tauroconjugated bile salts with broad substrate specificity
-
-
-
additional information
?
-
J7H3P9
broad substrate specificity
-
-
-
additional information
?
-
-
broad substrate specificity
-
-
-
additional information
?
-
-
the Lactobacillus salivarius enzyme displays potent hydrolysis activity towards both glycoconjugated and tauroconjugated bile salts with broad substrate specificity
-
-
-
additional information
?
-
-
oxgall is a substrate for the enzyme from different strains, overview
-
-
-
additional information
?
-
Lactobacillus spp.
-
enzyme activity is important for Lactobacillus colonization of human intestine
-
?
additional information
?
-
-
catalyzes the deconjugation of conjugated bile acids to liberate free primary bile acids and amino acids
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
glycodeoxycholic acid + H2O
3alpha,12alpha-dihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
G0YYC2
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
G0YYC2
about 75% activity compared to glycocholic acid
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
-
-
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
J7H3P9
best substrate
-
-
?
glycochenodeoxycholic acid + H2O
chenodeoxycholate + glycine
J7H3P9
best substrate
-
-
?
glycocholate + H2O
cholate + glycine
H6WSA2
preferred substrate
-
-
?
glycocholate + H2O
cholate + glycine
H6WSA2
preferred substrate
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
G0YYC2
100% activity
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
strain CK102
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine
-
-
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
G0YYC2
about 80% activity compared to glycocholic acid
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
isoform BSH1 shows much higher hydrolysis on glycodeoxycholic acid than isoforms BSH2, BSH3 and BSH4
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
-
best substrate
-
-
?
glycodeoxycholic acid + H2O
deoxycholate + glycine
J7H3P9
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
G0YYC2
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
G0YYC2
about 15% activity compared to glycocholic acid
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
J7H3P9
-
-
-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
J7H3P9
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
G0YYC2
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
G0YYC2
about 20% activity compared to glycocholic acid
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurocholic acid + H2O
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
G0YYC2
less than 20% activity compared to glycocholic acid
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
-
-
-
-
?
taurodeoxycholic acid + H2O
deoxycholate + taurine
J7H3P9
-
-
-
?
additional information
?
-
G0YYC2
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
G0YYC2
the enzyme exhibits clear preference for glycine-conjugated bile salts over taurine-conjugated forms
-
-
-
additional information
?
-
-
the enzyme catalyzes the deconjugation of glycine- or taurine-linked bile salts
-
-
-
additional information
?
-
-
BSH can hydrolyze all the six major human bile salts and at least two animal bile salts. BSH from five strains show a better deconjugation rate on glycine-conjugated bile salts than on taurine-conjugated forms
-
-
-
additional information
?
-
-
catalyzes the hydrolysis of glycine- and taurine-conjugated bile salts to amino acid residues and free bile salts
-
-
-
additional information
?
-
-
the enzyme hydrolyzes all of the six major human bile salts
-
-
-
additional information
?
-
-
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
Q2YS20
the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts
-
-
-
additional information
?
-
-
BSH catalyzes the hydrolysis of amide bond in conjugated bile salts and free amino acids are released, which form the deconjugated bile acid, mainly cholic and quenodeoxycholic
-
-
-
additional information
?
-
-
strain JCM1069 exhibits hydrolase activity against the taurodeoxycholic acid but not against the taurocholic acid, although both acids have taurine as their amino acid moiety but vary in their steroid moieties at 7alpha position
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
-
isoform BSH1 prefers deoxycholic salts over chenodeoxycholic and cholic acid salts
-
-
-
additional information
?
-
-
oxgall is a substrate for the enzyme from different strains, overview
-
-
-
additional information
?
-
Lactobacillus spp.
-
enzyme activity is important for Lactobacillus colonization of human intestine
-
?
additional information
?
-
-
catalyzes the deconjugation of conjugated bile acids to liberate free primary bile acids and amino acids
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Na+
additional information
additional information
poor activating effect at 30 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cholate
-
uncompetitive inhibition of glycocholic acid hydrolysis
phenylmethylsulfonyl fluoride
90.9% residual activity at 0.5 mM
Sodium periodate
-
about 99% inhibition at 0.1-2 mM, complete inhibition at 10 mM
taurine
-
uncompetitive inhibition of glycocholic acid hydrolysis
taurocholic acid
-
competitive inhibition of glycocholic acid hydrolysis
4-chloromercuriacetate
over 98% inhibition at 0.01 mM
-
4-chloromercuriacetate
over 98% inhibition at 0.01 mM
-
caffeic acid phenethyl ester
71.8% inhibition of recombinant enzyme at 5 mM, 80% at 10 mM, 50% at 03125 mM
demeclocycline
99.6% inhibition of recombinant enzyme at 2.5 mM
doxycycline
98.3% inhibition of recombinant enzyme at 2.5 mM
epicatechin monogallate
52.8% inhibition of recombinant enzyme at 5 mM
methacycline
99.2% inhibition of recombinant enzyme at 2.5 mM
oxytetracycline
99.6% inhibition of recombinant enzyme at 2.5 mM
Purpurogallin
36.1% inhibition of recombinant enzyme at 5 mM
riboflavin
96.5% inhibition of recombinant enzyme at 0.5 mM, 50% at 0.016 mM
additional information
-
BSH is vigorously inhibited by thiol enzyme inhibitors
-
additional information
-
the broad substrate specificity nature of Lactobacillus salivarius enzyme may make it an ideal candidate for screening desired BSH inhibitors targeting various BSH enzymes
-
additional information
-
identification of bile salt hydrolase inhibitors using an efficient high-throughput screening system, method optimization, overview; no inhibition by folic acid at 1.5 mM and bacitracin at 5 mM
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.028
glycochenodeoxycholate
-
pH 6.5, 40°C, recombinant wild-type enzyme
0.042
taurochenodeoxycholate
-
pH 6.5, 40°C, recombinant wild-type enzyme
0.91
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT
2.17
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT
15.02
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
15.04
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
0.5
glycocholic acid
-
pH 5.8-6.3, temperature not specified in the publication
0.00308
glycodeoxycholic acid
-
apparent value, calculated from a Lineweaver-Burk plot
0.00308
glycodeoxycholic acid
-
pH not specified in the publication, temperature not specified in the publication
11.88
glycodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
14.12
glycodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
2.5 - 3
taurochenodeoxycholic acid
-
pH 5.4, 37°C, recombinant enzyme
14.83
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
17.16
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
15.77
taurodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
18.12
taurodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
isozymes BSH1 and BSH2 are allosteric enzymes, and show positive cooperativity, catalytic efficiency and substrate preference, but with differences, overview. Michaelis-Menten kinetics in the presence of dithiothreitol, but not in its absence. Steady-state kinetics, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
33.52
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT
35.32
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT
53.82
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
83.77
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
48.76
glycodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
80.87
glycodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
632
glycodeoxycholic acid
-
apparent value, calculated from a Lineweaver-Burk plot
632
glycodeoxycholic acid
-
pH not specified in the publication, temperature not specified in the publication
91.53
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
93.11
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
83.15
taurodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
99.43
taurodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.58
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
5.57
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
16.82
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2, in presence of DTT
36.84
glycochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1, in presence of DTT
5.33
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH1
6.28
taurochenodeoxycholic acid
-
pH 6.5, 37°C, recombinant isozyme BSH2
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.86
4-amino-phenylacetic acid
-
pH 6.5, 40°C, recombinant wild-type enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4
-
pH 5.4, 37°C, purified recombinant enzyme, substrate glycodeoxycholic acid
5.6
-
pH 5.4, 37°C, purified recombinant enzyme, substrate taurocholic acid
7.7
-
pH 5.4, 37°C, purified recombinant enzyme, substrate glycocholic acid
8
-
pH 5.4, 37°C, purified recombinant enzyme, substrate taurochenodeoxycholic acid
8.5
-
pH 5.4, 37°C, purified recombinant enzyme, substrate taurodeoxycholic acid
17.7
-
pH 5.4, 37°C, purified recombinant enzyme, substrate glycochenodeoxycholic acid
additional information
additional information
very low activity in Bifidobacterium adolescentis strain DSM 20087
additional information
very high activity in Bifidobacterium animalis strain NRRL B-41405; very low activity in Bifidobacterium animalis strain NRRL B-41406
additional information
I3QNS9
very high activity in Bifidobacterium animalis strain NRRL B-41405; very low activity in Bifidobacterium animalis strain NRRL B-41406
additional information
-
bile salt hydrolase activity 1.0 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
low activity in Bifidobacterium bifidum strain DSM 20456
additional information
low activity in Bifidobacterium breve strain DSM 20091; low activity in Bifidobacterium breve strain NRRL B-41408
additional information
-
bile salt hydrolase activity 1.1 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
high activity in Bifidobacterium longum strain NRRL B-41407; low activity in Bifidobacterium longum strain ATCC 15697; low activity in Bifidobacterium longum subsp. longum strain NRRL B-41409
additional information
-
high activity in Bifidobacterium longum strain NRRL B-41407; low activity in Bifidobacterium longum strain ATCC 15697; low activity in Bifidobacterium longum subsp. longum strain NRRL B-41409
additional information
high activity in Bifidobacterium longum strain NRRL B-41407; low activity in Bifidobacterium longum strain ATCC 15697; low activity in Bifidobacterium longum subsp. longum strain NRRL B-41409
additional information
-
bile salt hydrolase activity 1.0 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information
-
lower activity in Bifidobacterium pseudocatenulatum strain DSM 20224; moderate activity in Bifidobacterium pseudocatenulatum strain DSM 20439
additional information
lower activity in Bifidobacterium pseudocatenulatum strain DSM 20224; moderate activity in Bifidobacterium pseudocatenulatum strain DSM 20439
additional information
very low activity in Bifidobacterium pseudolongum strain DSM 20095
additional information
-
glycocholic acid 2.2 U, glycodeoxycholic acid 2.7 U, glycochenodeoxycholic acid 1.7 U, taurocholic acid 1.7 U, taurodeoxycholic acid 1.7 U, taurochenodeoxycholic acid 1.7 U
additional information
-
BSH activity 1.9 Dp(cm), determined by the plate assay method, Dp, diameter of the precipitation zone
additional information