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EC Tree
The taxonomic range for the selected organisms is: Escherichia coli The enzyme appears in selected viruses and cellular organisms
Synonyms
glutaminase, l-glutaminase, phosphate-activated glutaminase, mitochondrial glutaminase, glutaminase 1, kidney-type glutaminase, phosphate activated glutaminase, glutaminase a, glnase, glutaminase c,
more
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glutamine aminohydrolase
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L-glutamine amidohydrolase
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phosphate activated glutaminase
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L-glutamine + H2O = L-glutamate + NH3
mechanism
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carboxylic acid amide hydrolysis
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L-glutamine amidohydrolase
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L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
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-
-
?
L-glutamine + H2O
L-glutamic acid + NH3
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-
-
?
gamma-ethyl glutamate + H2O
Glu + ethanol
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-
-
-
?
gamma-ethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ethanol
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-
-
-
?
gamma-glutamyl-hydrazide + H2O
Glu + hydroxyhydrazine
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-
-
-
?
gamma-glutamyl-hydrazide + hydroxylamine
Glu + hydroxyhydrazine
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-
-
-
?
gamma-glutamyl-hydroxamate + H2O
Glu + hydroxylamine
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-
-
-
?
gamma-glutamyl-hydroxamate + hydroxylamine
gamma-glutamyl hydroxamate + hydroxylamine
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-
-
-
?
gamma-glutamyl-methoxyamide + H2O
Glu + N-hydroxy-O-methylhydroxylamine
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-
-
-
?
gamma-glutamyl-methoxyamide + hydroxylamine
gamma-glutamyl hydroxamate + N-hydroxy-O-methylhydroxylamine
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-
-
-
?
gamma-glutamyl-methylamide + H2O
Glu + N-methylhydroxylamine
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-
-
-
?
gamma-glutamyl-methylamide + hydroxylamine
gamma-glutamyl hydroxamate + N-methylhydroxylamine
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-
-
-
?
gamma-methyl glutamate + H2O
Glu + methanol
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-
-
r
gamma-methyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + methanol
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-
-
-
?
gamma-thioethyl glutamate + H2O
Glu + thioethanol
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-
-
-
?
gamma-thioethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ?
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-
-
-
?
gamma-thiomethyl glutamate + H2O
Glu + thiomethanol
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-
-
-
?
gamma-thiomethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ?
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-
-
-
?
Gln + H2O
Glu + NH3
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-
-
-
?
Glu + hydroxylamine
gamma-glutamyl hydroxamate + H2O
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-
-
-
?
L-Gln + hydroxylamine
L-gamma-glutamyl hydroxamate + NH3
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-
-
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
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-
-
?
L-glutamine + H2O
L-glutamate + NH3
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?
L-glutamine + H2O
L-glutamic acid + NH3
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?
nitrocefin + H2O
(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
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-
-
?
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6-diazo-5-oxo-L-norleucine
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6-diazo-5-oxo-L-norleucine
Hg2+
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0.1 mM, complete inhibition
Ngamma,Ngamma-diethyl-L-glutamine
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Ngamma,Ngamma-dimethyl-L-glutamine
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Ngamma-ethyl-L-glutamine
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Ngamma-methyl-L-glutamine
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p-mercuribenzoate
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0.1 mM, complete inhibition, presence of Gln prevents inhibition
6-diazo-5-oxo-L-norleucine
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6-diazo-5-oxo-L-norleucine
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1,10-phenanthroline
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slight activation
dithiothreitol
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slight activation
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50
gamma-ethyl glutamate
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-
12
gamma-glutamyl hydrazide
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3.3
gamma-glutamyl methylamide
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64
gamma-methyl glutamate
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-
23
gamma-thioethyl glutamate
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10
gamma-thiomethyl glutamate
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-
additional information
additional information
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effect of pH on Km-value
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7.3
L-glutamine
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8.5
L-glutamine
mutant Q162A
23.7
L-glutamine
mutant G261A
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36
gamma-ethyl glutamate
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-
14
gamma-glutamyl hydrazide
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-
296
gamma-glutamyl methoxyamide
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-
8
gamma-glutamyl methylamide
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-
212
gamma-glutamyl-hydroxamate
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645
gamma-methyl glutamate
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-
300
gamma-thioethyl glutamate
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-
1260
gamma-thiomethyl glutamate
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-
additional information
additional information
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effect of pH on turnover number
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49.9
L-glutamine
mutant Q161A
79.9
L-glutamine
mutant Q162A
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3.5
ampicillin
competitive inhibitor
2.95
Ngamma,Ngamma-diethyl-L-glutamine
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37°C, pH 7.5
2.64
Ngamma,Ngamma-dimethyl-L-glutamine
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37°C, pH 7.5
1.58
Ngamma-ethyl-L-glutamine
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37°C, pH 7.5
1.11
Ngamma-methyl-L-glutamine
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37°C, pH 7.5
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1.7 - 5.5
Mg2+
Escherichia coli
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0.7 - 1.3
Mn2+
Escherichia coli
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17.2 - 17.4
PO43-
Escherichia coli
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0.2
Mg2+
Escherichia coli
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0.1
Mn2+
Escherichia coli
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39
Ngamma,Ngamma-diethyl-L-glutamine
Escherichia coli
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37°C, pH 7.5
35
Ngamma,Ngamma-dimethyl-L-glutamine
Escherichia coli
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37°C, pH 7.5
24
Ngamma-ethyl-L-glutamine
Escherichia coli
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37°C, pH 7.5
20
Ngamma-methyl-L-glutamine
Escherichia coli
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37°C, pH 7.5
17.2 - 17.4
PO43-
Escherichia coli
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additional information
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7.1 - 9
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hydrolysis of Gln, glutaminase B
8.3 - 9.2
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formation of gamma-glutamyl hydroxamate from L-Gln and NH2OH
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6 - 11
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pH 6.0: about 55% of maximal activity, pH 11.0: about 55% of maximal activity, hydrolysis of Gln
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SwissProt
brenda
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brenda
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33500
2 * 33500, SDS-PAGE
32900
4 * 32900, SDS-PAGE
90000
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glutaminase B, gel filtration
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homodimer
2 * 33500, SDS-PAGE
homotetramer
4 * 32900, SDS-PAGE
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G261A
54,7% wild-type activity, Km: 23.7 mM
Q162A
wild-type level of activity
S260A
16.5% of wild-type activity
Y29A
32.5% of wild-type activity
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4
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low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity
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significant loss of activity upon freezing at -20°C and -80°C in presence and absence of 20% glycerol
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sodium borate is the most effective stabilizing agent against cold inactivation
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4°C, in presence of sodium borate, stable for 5 days
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stable at -196°C (unstable at -80°C)
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analysis
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construction of a L-glutaminase enzyme reactor based on immobilization of enzyme onto bamboo sticks through a glutaraldehyde modification to achieve covalent bonding. L-glutaminase-bamboo exhibits improved enzymatic hydrolysis performances, including high hydrolysis efficiency, prolonged stability (14 days) and good reusability and can efficiently be uesd for inhibitor screening
medicine
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acivicin along with Escherichia coli glutaminase synergistically reduces in vitro proliferation and matrigel invasion of human MCF-7 and OAW-42 cells. Combination of acivicin with glutaminase may provide a better therapeutic option than either of them separately for treating human breast and ovarian cancer
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Prusiner, S.; Davis, J.N.; Stadtman, E.R.
Regulation of glutaminase B in Escherichia coli. I. Purification, properties, and cold lability
J. Biol. Chem.
251
3447-3456
1976
Escherichia coli
brenda
Hartman, S.C.
Glutaminases and gamma-glutamyltransferases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
79-100
1971
Escherichia coli
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brenda
Roy, S.; Ghosh, S.; Mallick, P.; Maity, P.
Acivicin with glutaminase regulates proliferation and invasion of human MCF-7 and OAW-42 cells-an in vitro study
Indian J. Exp. Biol.
46
22-26
2008
Escherichia coli
brenda
Brown, G.; Singer, A.; Proudfoot, M.; Skarina, T.; Kim, Y.; Chang, C.; Dementieva, I.; Kuznetsova, E.; Gonzalez, C.F.; Joachimiak, A.; Savchenko, A.; Yakunin, A.F.
Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis
Biochemistry
47
5724-5735
2008
Bacillus subtilis (O07637), Bacillus subtilis (O31465), Bacillus subtilis, Escherichia coli (P0A6W0), Escherichia coli (P77454), Escherichia coli, Bacillus subtilis 168 (O07637), Bacillus subtilis 168 (O31465), Escherichia coli W3110 / ATCC 27325 (P0A6W0), Escherichia coli W3110 / ATCC 27325 (P77454)
brenda
Prokop, M.; Czarnecka, J.; Milewska, M.
Ngamma-alkyl derivatives of L-glutamine as inhibitors of glutamine-utilizing enzymes
Z. Naturforsch. C
64
631-636
2009
Escherichia coli
brenda
Qiao, J.; Zhao, L.; Liu, L.; Qi, L.
An L-glutaminase enzyme reactor based on porous bamboo sticks and its application in enzyme inhibitors screening
Talanta
205
120126
2019
Escherichia coli
brenda