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Information on EC 3.5.1.118 - gamma-glutamyl hercynylcysteine S-oxide hydrolase and Organism(s) Mycolicibacterium smegmatis and UniProt Accession A0R5M9

for references in articles please use BRENDA:EC3.5.1.118
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IUBMB Comments
The enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria.
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This record set is specific for:
Mycolicibacterium smegmatis
UNIPROT: A0R5M9
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The taxonomic range for the selected organisms is: Mycolicibacterium smegmatis
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
EgtC, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-gamma-glutamyl hercynylcysteine S-oxide amidohydrolase
The enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O
S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-(hercyn-2-yl)-gamma-L-glutamyl-L-cysteine S-oxide + H2O
S-(hercyn-2-yl)-L-cysteine S-oxide + L-glutamate
show the reaction diagram
the enzyme is part of the biosynthesis pathway of ergothioneine in mycobacteria
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24139
x * 24139, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 24139, calculated from sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion method
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Seebeck, F.P.
In vitro reconstitution of Mycobacterial ergothioneine biosynthesis
J. Am. Chem. Soc.
132
6632-6633
2010
Mycolicibacterium smegmatis (A0R5M9), Mycolicibacterium smegmatis ATCC 700084 (A0R5M9)
Manually annotated by BRENDA team
Vit, A.; Mashabela, G.; Blankenfeldt, W.; Seebeck, F.
Structure of the ergothioneine-biosynthesis amidohydrolase EgtC
ChemBioChem
16
1490-1496
2015
Mycolicibacterium smegmatis (A0R5M9), Mycolicibacterium smegmatis ATCC 700084 (A0R5M9)
Manually annotated by BRENDA team