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Information on EC 3.5.1.115 - mycothiol S-conjugate amidase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WJN1

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IUBMB Comments
The enzyme that is found in actinomycetes is involved in the detoxification of oxidizing agents and electrophilic antibiotics. The enzyme has low activity with 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol as substrate (cf. EC 3.5.1.103, N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase) .
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Mycobacterium tuberculosis
UNIPROT: P9WJN1
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The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
mycothiol s-conjugate amidase, rv1082, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
mycothiol S-conjugate 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranosyl-hydrolase
The enzyme that is found in actinomycetes is involved in the detoxification of oxidizing agents and electrophilic antibiotics. The enzyme has low activity with 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol as substrate (cf. EC 3.5.1.103, N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase) [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a mycothiol S-conjugate + H2O
an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
show the reaction diagram
mycothiol bimane + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol disulfide + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-3-(N-maleimidopropionyl)biocytin + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-acetophenone + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-cerulenin + H2O
?
show the reaction diagram
-
-
-
-
?
mycothiol-N-ethylmaleimide + H2O
?
show the reaction diagram
-
-
-
-
?
RifS13 + H2O
1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,6,9,17,19-pentahydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,11-dioxo-1,2-dihydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
show the reaction diagram
-
-
-
-
?
RifS17 + H2O
1-D-myo-inositol-alpha-D-glucopyranoside + N-acetyl-S-[(2R,12Z,14E,16S,18S,19S,20S,21S,22S,23S,24E)-5,17,19-trihydroxy-23-methoxy-21-(methoxycarbonyl)-2,4,12,16,18,20,22-heptamethyl-1,6,9,11-tetraoxo-1,2,6,9-tetrahydro-2,7-(epoxypentadeca[1,11,13]trienoimino)naphtho[2,1-b]furan-8-yl]-L-cysteine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme is highly specific for the mycothiol moiety of mycothiol S-conjugates and relatively nonspecific for the structure of the sulfur-linked conjugate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a mycothiol S-conjugate + H2O
an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
the protein contains 2.6 equivalents of Ca2+ per subunit after purification
Fe2+
-
metalloamidase. The enzyme purifies with (stoichiometric) Fe2+ when purified under anaerobic conditions, and Zn2+ when purified under aerobic conditions. It is likely a Fe2+-dependent enzyme under physiological conditions, with Zn2+-enzyme an experimental artifact that can become biologically relevant under oxidatively stressed conditions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arenosclerin E
homology model built for Mca protein of Mycobacterium tuberculosis have high reliability and docking analysis show that arenosclerin E is a potent drug candidate for tuberculosis
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-({[3-chloro-4-(propan-2-ylsulfonyl)thiophen-2-yl]carbonyl}amino)-2-deoxy-alpha-D-glucopyranoside
-
11% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-({[1-(3,5-dichlorophenyl)-5-ethyl-1H-pyrazol-4-yl]carbonyl}amino)-alpha-D-glucopyranoside
-
26% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(4-nitrobenzoyl)amino]-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(furan-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(naphthalen-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(pyridin-4-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
46% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-[(thiophen-2-ylcarbonyl)amino]-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[3-(propylsulfanyl)benzoyl]amino}-alpha-D-glucopyranoside
-
less than 10% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[4-(dimethylamino)benzoyl]amino}-alpha-D-glucopyranoside
-
44% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-deoxy-2-{[4-(trifluoromethyl)benzoyl]amino}-alpha-D-glucopyranoside
-
16% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[(1-benzothiophen-6-ylcarbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
15% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[(cyclopropylcarbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
13% inhibition at 0.05 mM
(1R,2R,3R,5S)-2,3,5-trihydroxy-5-(hydroxymethyl)cyclohexyl 2-[({6-[(4-chlorophenyl)sulfanyl]pyridin-2-yl}carbonyl)amino]-2-deoxy-alpha-D-glucopyranoside
-
10% inhibition at 0.05 mM
(2E)-3-(3,5-difluorophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-bromophenyl)-N-[2-[(2,6-dimethylphenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-bromophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-3-(3-chloro-4-hydroxyphenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-(5-amino-5-iminopentyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3,5-difluorophenyl)-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-bromophenyl)-2-(hydroxyimino)propanamide
-
-
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-chloro-4-hydroxyphenyl)-2-(hydroxyimino)propanamide
-
-
(2S,3R,26S,27S)-2,27-diamino-1,3,26-trihydroxyoctacosan-13-one
-
-
(2Z)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-N-(4-carbamimidamidobutyl)-2-(hydroxyimino)propanamide
-
-
(2Z)-N-(4-amino-4-iminobutyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2Z)-N-[(2-amino-1H-imidazol-5-yl)methyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(2Z)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
-
-
(5R,10S)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
-
-
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
-
-
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]}-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
-
strongest inhibitor
1,10-phenanthroline
-
0.19% residual activity at 1 mM 1,10-phenanthroline
1,7-phenanthroline
-
slightly more than 50% inhibition at 1 mM 1,7-phenanthroline
1-pentyl-2-[10-(3-pentylpyridinium-1-yl)decyl]pyridinium
-
-
3,5,8-trihydroxyquinolin-4(1H)-one
-
-
bromotyrosine oxime
-
-
disodium 3-[(E)-2-carboxylatoethenyl]benzoate
-
-
gliotoxin
-
mixed non-competitive inhibitor
hemibastidin-2
-
-
mycothiol
-
about 20% residual activity at 2 mM mycothiol
oceanapiside
-
low micromolar, non-competitive inhibitor
pseudoceratine
-
-
S,S-dimethyl gliotoxin
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8
mycothiol disulfide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
3
mycothiol-3-(N-maleimidopropionyl)biocytin
-
Km above 3.0 mM, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.44
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.69
mycothiol-acetophenone
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.65
mycothiol-cerulenin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
1.3
mycothiol-N-ethylmaleimide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.19
RifS13
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.45
RifS17
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
mycothiol disulfide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
3
mycothiol-3-(N-maleimidopropionyl)biocytin
-
kcat above 3.0 s-1, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
1.3
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
37
mycothiol-acetophenone
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
7
mycothiol-cerulenin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
15
mycothiol-N-ethylmaleimide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
0.82
RifS13
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
1.04
RifS17
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
mycothiol disulfide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
1.03
mycothiol-3-(N-maleimidopropionyl)biocytin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
3
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
54
mycothiol-acetophenone
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
11
mycothiol-cerulenin
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
12
mycothiol-N-ethylmaleimide
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
4.3
RifS13
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
2.3
RifS17
-
in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.72
(2E)-3-(3,5-difluorophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.065
(2E)-3-(3-bromophenyl)-N-[2-[(2,6-dimethylphenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.09
(2E)-3-(3-bromophenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.037
(2E)-3-(3-chloro-4-hydroxyphenyl)-N-[2-[(4-fluorophenyl)disulfanyl]ethyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0028
(2E)-N-(5-amino-5-iminopentyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.036
(2E)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.45
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3,5-difluorophenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.185
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-bromophenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.035
(2E)-N-[2-[(4-aminophenyl)disulfanyl]ethyl]-3-(3-chloro-4-hydroxyphenyl)-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.1
(2S,3R,26S,27S)-2,27-diamino-1,3,26-trihydroxyoctacosan-13-one
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.003
(2Z)-N-(4-amino-4-iminobutyl)-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.03
(2Z)-N-[(2-amino-1H-imidazol-5-yl)methyl]-3-[4-(3-aminopropoxy)-3,5-dibromophenyl]-2-(hydroxyimino)propanamide
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.1
(5R,10S)-N-[2-(2-amino-1H-imidazol-5-yl)ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.002
(5R,10S)-N-[2-[2-amino-4-(3,5,8-trihydroxy-4-oxo-1,4-dihydroquinolin-6-yl)-1H-imidazol-5-yl]ethyl]-7,9-dibromo-10-hydroxy-8-methoxy-1-oxa-2-azaspiro[4.5]deca-2,6,8-triene-3-carboxamide
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0001
1-pentyl-2-[10-(3-pentylpyridinium-1-yl)decyl]pyridinium
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.03
bromotyrosine oxime
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.003
disodium 3-[(E)-2-carboxylatoethenyl]benzoate
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.05
gliotoxin
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.01
oceanapiside
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
0.0028
psammaplin A
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.02 - 0.03
psammaplysin A
0.026 - 0.03
psammaplysin B
0.1
pseudoceratine
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.07
S,S-dimethyl gliotoxin
Mycobacterium tuberculosis
-
at 31°C, pH not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.13
-
crude extract, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
10.6
-
after 82fold purification, in 50 mM HEPES (pH 7.5) containing 50 mM NaCl and 0.1 mM dithiothreitol, at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
mycothiol serves as the primary reducing agent in Mycobacterium species, and is also a cofactor for the detoxification of xenobiotics. The enzyme catalyzes the cleavage of mycothiol-S-conjugates to form a mercapturic acid, which is excreted from the mycobacterium
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32697
-
x * 32697, calculated from amino acid sequence
33000
-
x * 33000, SDA-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
the gene is cloned into the pVP56 K [N-terminal His-maltose-binding protein (MBP)] and pFN18 K (N-terminal Halo-tag) expression vectors, expression in Escherichia coli BL21(DE3) cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
homology model built for Mca protein of Mycobacterium tuberculosis have high reliability and docking analysis show that Arenosclerin E is a potent drug candidate for tuberculosis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Metaferia, B.B.; Ray, S.; Smith, J.A.; Bewley, C.A.
Design and synthesis of substrate-mimic inhibitors of mycothiol-S-conjugate amidase from Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
17
444-447
2007
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Steffek, M.; Newton, G.L.; Av-Gay, Y.; Fahey, R.C.
Characterization of Mycobacterium tuberculosis mycothiol S-conjugate amidase
Biochemistry
42
12067-12076
2003
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Manually annotated by BRENDA team
Nicholas, G.M.; Eckman, L.L.; Ray, S.; Hughes, R.O.; Pfefferkorn, J.A.; Barluenga, S.; Nicolaou, K.C.; Bewley, C.A.
Bromotyrosine-derived natural and synthetic products as inhibitors of mycothiol-S-conjugate amidase
Bioorg. Med. Chem. Lett.
12
2487-2490
2002
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Fetterolf, B.; Bewley, C.A.
Synthesis of a bromotyrosine-derived natural product inhibitor of mycothiol-S-conjugate amidase
Bioorg. Med. Chem. Lett.
14
3785-3788
2004
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Nicholas, G.M.; Eckman, L.L.; Newton, G.L.; Fahey, R.C.; Ray, S.; Bewley, C.A.
Inhibition and kinetics of mycobacterium tuberculosis and Mycobacterium smegmatis mycothiol-S-conjugate amidase by natural product inhibitors
Bioorg. Med. Chem.
11
601-608
2003
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
Manually annotated by BRENDA team
Nicholas, G.M.; Newton, G.L.; Fahey, R.C.; Bewley, C.A.
Novel bromotyrosine alkaloids: inhibitors of mycothiol S-conjugate amidase
Org. Lett.
3
1543-1545
2001
Mycobacterium tuberculosis
Manually annotated by BRENDA team
Saxena, A.; Mishra, S.
Marine sponge derived natural products as inhibitors of mycothiol-S-conjugate amidase
Bioinformation
13
256-260
2017
Mycobacterium tuberculosis (P9WJN1), Mycobacterium tuberculosis ATCC 25618 (P9WJN1)
Manually annotated by BRENDA team
Kocabas, E.; Liu, H.; Hernick, M.
Identity of cofactor bound to mycothiol conjugate amidase (Mca) influenced by expression and purification conditions
Biometals
28
755-763
2015
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
Manually annotated by BRENDA team