We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
L-amino acid amidase, LaaA, S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase
L-amino acid amidase
-
LaaA
-
S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase
-
S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-piperidine-2-carboxamide + H2O = (S)-piperidine-2-carboxylate + NH3
-
-
-
-
L-prolinamide + H2O = L-proline + NH3
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-piperidine-2-carboxamide amidohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R,S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-tert-butylcarboxylic acid + NH3
(S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + NH3
(S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-carboxylic acid + tert-butylamine
0.2% of the activity with L-proline amide
-
-
?
(S)-piperidine-2-carboxamide + H2O
(S)-piperidine-2-carboxylic acid + NH3
32% of the activity with L-proline amide
-
-
?
L-alanine amide + H2O
L-alanine + NH3
10.6% of the activity with L-proline amide
-
-
?
L-isoleucine amide + H2O
L-isoleucine + NH3
0.17% of the activity with L-proline amide
-
-
?
L-leucine amide + H2O
L-leucine + NH3
L-methionine amide + H2O
L-methionine + NH3
4.2% of the activity with L-proline amide
-
-
?
L-phenylalanine amide + H2O
L-phenylalanine + NH3
L-proline amide + H2O
L-proline + NH3
-
-
-
?
L-proline-p-nitroanilide + H2O
? + NH3
40.9% of the activity with L-proline amide
-
-
?
L-serine amide + H2O
L-serine + NH3
0.43% of the activity with L-proline amide
-
-
?
L-threonine amide + H2O
L-threonine + NH3
0.12% of the activity with L-proline amide
-
-
?
L-tryptophan amide + H2O
L-tryptophan + NH3
0.2% of the activity with L-proline amide
-
-
?
L-tyrosine amide + H2O
L-tyrosine + NH3
0.086% of the activity with L-proline amide
-
-
?
additional information
?
-
(R,S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-tert-butylcarboxylic acid + NH3
% of the activity with L-proline amide
-
-
?
(R,S)-piperazine-2-tert-butylcarboxamide + H2O
(S)-piperazine-2-tert-butylcarboxylic acid + NH3
% of the activity with L-proline amide
-
-
?
(S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + NH3
3.7% of the activity with L-proline amide
-
-
?
(S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + NH3
3.7% of the activity with L-proline amide
-
-
?
L-leucine amide + H2O
L-leucine + NH3
0.46% of the activity with L-proline amide
-
-
?
L-leucine amide + H2O
L-leucine + NH3
0.46% of the activity with L-proline amide
-
-
?
L-phenylalanine amide + H2O
L-phenylalanine + NH3
0.97% of the activity with L-proline amide
-
-
?
L-phenylalanine amide + H2O
L-phenylalanine + NH3
0.97% of the activity with L-proline amide
-
-
?
additional information
?
-
the following compounds are not substrates for the amidase: L-arginine amide, L-asparagine amide, L-isoasparagine, L-glutaminamide, L-isoglutamine, glycine amide, L-histidine amide, L-lysine amide, L-valine amide, D-proline amide, L-alanyl-L-alanine, L-alanylglycine, glycylglycine, L-prolyl-L-alanine and L-prolylglycine. LaaA can not act on the peptide substrates such as L-prolyl-L-alanine, L-prolylglycine, L-alanyl-L-alanine, L-alanylglycine and glycylglycine
-
-
?
additional information
?
-
the following compounds are not substrates for the amidase: L-arginine amide, L-asparagine amide, L-isoasparagine, L-glutaminamide, L-isoglutamine, glycine amide, L-histidine amide, L-lysine amide, L-valine amide, D-proline amide, L-alanyl-L-alanine, L-alanylglycine, glycylglycine, L-prolyl-L-alanine and L-prolylglycine. LaaA can not act on the peptide substrates such as L-prolyl-L-alanine, L-prolylglycine, L-alanyl-L-alanine, L-alanylglycine and glycylglycine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ag+
1 mM, complete inhibition
Cd2+
1 mM, complete inhibition
CoCl2
1 mM, 52% inhibition
Hg2+
1 mM, complete inhibition
iodoacetate
1 mM, 40% inhibition
NiCl2
1 mM, 70% inhibition
p-chloromercuribenzoate
1 mM, 67% inhibition
PbCl2
1 mM, 73% inhibition
phenylhydrazine
1 mM, complete inhibition
Zn2+
1 mM, complete inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.58
L-proline-p-nitroanilide
30°C, pH 7.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.000894
S-stereoselective amidase from Pseudomonas azotoformans IAM 1603
192
LaaA from Escharichia coli JM109 harboring pSTB20
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IAM 1603
SwissProt
brenda
IAM 1603
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
34000
1 * 34000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
monomer
1 * 34000, SDS-PAGE
monomer
-
1 * 34000, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, stored without loss of activity for more than six months in the buffer containing 50% glycerol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
synthesis
Escherichia coli cells overexpressing the laaA gene have been demonstrated to be applicable to the S-stereoselective hydrolysis of (R,S)-piperazine-2-tert-butylcarboxamide to produce (S)-piperazine-2-carboxylic acid with high optical purity. Enantiomerically pure piperazine-2-carboxylic acid and its tert-butylcarboxamide derivative are important chiral building blocks for some pharmacologically active compounds such as N-methyl-D-aspartate antagonist for glutamate receptor, cardioprotective nucleoside transport blocker and HIV protease inhibitor
synthesis
-
Escherichia coli cells overexpressing the laaA gene have been demonstrated to be applicable to the S-stereoselective hydrolysis of (R,S)-piperazine-2-tert-butylcarboxamide to produce (S)-piperazine-2-carboxylic acid with high optical purity. Enantiomerically pure piperazine-2-carboxylic acid and its tert-butylcarboxamide derivative are important chiral building blocks for some pharmacologically active compounds such as N-methyl-D-aspartate antagonist for glutamate receptor, cardioprotective nucleoside transport blocker and HIV protease inhibitor
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Komeda, H.; Harada, H.; Washika, S.; Sakamoto, T.; Ueda, M.; Asano, Y.
S-stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase
Eur. J. Biochem.
271
1465-1475
2004
Pseudomonas azotoformans (Q76KX0), Pseudomonas azotoformans IAM 1603 (Q76KX0)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
3.5.1.101 L-proline amide hydrolase
more
top
Information
