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Information on EC 3.4.24.B12 - ADAMTS5 endopeptidase and Organism(s) Bos taurus and UniProt Accession Q9TT92

for references in articles please use BRENDA:EC3.4.24.B12
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B12 ADAMTS5 endopeptidase
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This record set is specific for:
Bos taurus
UNIPROT: Q9TT92
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
proteolytic degradation of proteins
Synonyms
adamts-5, adamts5, aggrecanase-2, adam-ts5, aggrecanase 2, aggrecanase-, agg-2, adamts11, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
a disintegrin and metalloproteinase with thrombospondin motifs 5
-
ADAM-TS 5
-
ADAM-TS11
-
ADAM-TS5
-
ADAMTS-5
-
aggrecanase-2
-
M12.225
Merops-ID
a disintegrin and metalloproteinase with thrombospondin motifs 5
-
-
a disintegrin and metalloproteinase with thrombospondin motifs-5
-
-
ADAMTS-5
-
-
ADAMTS11
-
-
ADAMTS5
-
-
aggrecanase-2
-
-
implantin
-
-
M12.225
-
Merops-ID
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
405150-12-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aggrecan + H2O
?
show the reaction diagram
proteins + H2O
peptides
show the reaction diagram
aggrecan + H2O
2 aggrecan fragment
show the reaction diagram
aggrecan + H2O
?
show the reaction diagram
-
-
-
-
?
proteins + H2O
peptides
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aggrecan + H2O
?
show the reaction diagram
enzyme may play a role in cartilage breakdown
-
?
proteins + H2O
peptides
show the reaction diagram
aggrecan + H2O
2 aggrecan fragment
show the reaction diagram
-
cleavage site is Glu373-Ala374, reaction is relevant to cartilage degeneration in inflammatory joint diseases
-
?
proteins + H2O
peptides
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha2-Macroglobulin
-
-
-
TIMP-3
-
potent inhibitor
-
additional information
-
TIMP-1, TIMP-2 and TIMP-4 do not inhibit ADAMTS-5
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
interleukin-1alpha
-
increased transcription in the presence of interleukin-1alpha
-
interleukin-1beta
-
increased transcription in the presence of interleukin-1beta
-
tissue necrosis factor alpha
-
increased transcription in the presence of tissue necrosis factor alpha
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
gene expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
disruptive changes in chondrocyte-matrix interactions by hyaluran oligosaccharides induce matrix degradation and elevate aggrecanases ADAMTS4, ADAMTS5 via the activation of the NF-kappaB signaling pathway
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ATS5_BOVIN
207
0
22576
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
x * 60000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 60000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
cleavage by furin
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Drosophila S2 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ADAMTS5 protein within cartilage increases following injury and with coculture
-
disruption of chondrocyte hyaluran receptor CD44-hyaluran interactions with hyaluran oligosaccharides induces the transcription of endopeptidases ADAMTS-4 and ADAMTS-5 in a time- and dose-dependent manner
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Bos taurus (Q9TT92), Homo sapiens (Q9UNA0), Mus musculus (Q9R001)
Manually annotated by BRENDA team
Vankemmelbeke, M.N.; Holen, I.; Wilson, A.G.; Ilic, M.Z.; Handley, C.J.; Kelner, G.S.; Clark, M.; Liu, C.; Maki, R.A.; Burnett, D.; Buttle, D.J.
Expression and activity of ADAMTS-5 in synovium
Eur. J. Biochem.
268
1259-1268
2001
Homo sapiens, Bos taurus (Q9TT92), Bos taurus
Manually annotated by BRENDA team
Tang, B.L.
ADAMTS: a novel family of extracellular matrix proteases
Int. J. Biochem. Cell Biol.
33
33-44
2001
Bos taurus
Manually annotated by BRENDA team
Fosang, A.J.; Rogerson, F.M.; East, C.J.; Stanton, H.
ADAMTS-5: the story so far
Eur. Cell Mater.
15
11-26
2008
Bos taurus, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Lee, J.H.; Fitzgerald, J.B.; DiMicco, M.A.; Cheng, D.M.; Flannery, C.R.; Sandy, J.D.; Plaas, A.H.; Grodzinsky, A.J.
Co-culture of mechanically injured cartilage with joint capsule tissue alters chondrocyte expression patterns and increases ADAMTS5 production
Arch. Biochem. Biophys.
489
118-126
2009
Bos taurus
Manually annotated by BRENDA team
Ariyoshi, W.; Takahashi, N.; Hida, D.; Knudson, C.; Knudson, W.
Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides
Arthritis Rheumatol.
64
187-197
2012
Bos taurus
Manually annotated by BRENDA team