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Information on EC 3.4.24.85 - S2P endopeptidase and Organism(s) Cricetulus griseus and UniProt Accession O54862

for references in articles please use BRENDA:EC3.4.24.85
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.85 S2P endopeptidase
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This record set is specific for:
Cricetulus griseus
UNIPROT: O54862 not found.
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Word Map
The taxonomic range for the selected organisms is: Cricetulus griseus
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
Cleaves several transcription factors that are type-2 transmembrane proteins within membrane-spanning domains. Known substrates include sterol regulatory element-binding protein (SREBP) -1, SREBP-2 and forms of the transcriptional activator ATF6. SREBP-2 is cleaved at the site DRSR_ILL_483-/-CVLTFLCLSFNPLTSLLQWGGA, in which the membrane-spanning segment is underlined. The residues NP (bold), 11 residues distal to the site of cleavage in the membrane-spanning domain, are important for cleavage by S2P endopeptidase. Replacement of either of these residues does not prevent cleavage, but there is no cleavage if both of these residues are replaced.
Synonyms
sterol regulatory element binding protein, spoivfb, site-2 protease, intramembrane-cleaving protease, i-clip, sll0528, slr0643, sterol-regulated protease, site-2-protease, site-2 metalloprotease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteinase, sterol regulatory element-binding protein
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site-1 protease
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site-2 protease
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SREBP cleavage activity
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SREBP cysteine proteinase
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SREBP proteinase
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SREBP-1 proteinase
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SREBP-2 proteinase
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sterol regulatory element-binding proteinase
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sterol-regulated protease
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
167140-48-9
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752251-31-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATF6 + H2O
?
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATF6 + H2O
?
show the reaction diagram
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Chinese hamster, s2p cloned
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
CHO-7 and M19 S2P deficient cell lines
Manually annotated by BRENDA team
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CHO-K1 wild type and M19 S2P deficient cell lines
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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cells defective in the site-2 protease gene have remarkably higher level of superoxide and elevated rates of cell death than wild-type CHO cells. Lack of the site-2 proease gene leads to cells more vulnerable to oxidative stress. Compared with wild-type CHO cells, mutant cells have higher nicotinamide adenine dinucleotide phosphate oxidase activity and lower paraoxonase-2 expression
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MBTP2_CRIGR
510
7
56499
Swiss-Prot
Secretory Pathway (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
M19 mutant cell line of CHO-K1 cells with no s2p activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in CHO cell line
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
oxidative stress significantly up-regulates site-2 protease expression in CHO cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rawson, R.; Zelenski, N.; Nijhawan, D.; Ye, J.; Sakai, J.; Hasan, M.; Chang, T.; Brown, M.; Goldstein, J.
Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs
Mol. Cell
1
47-57
1997
Homo sapiens (O43462), Cricetulus griseus (O54862)
Manually annotated by BRENDA team
Lee, K.; Tirasophon, W.; Shen, X.; Michalak, M.; Prywes, R.; Okada, T.; Yoshida, H.; Mori, K.; Kaufman, R.
IRE1-mediated unconventional mRNA splicing and S2P-mediated ATF6 cleavage merge to regulate XBP1 in signaling the unfolded protein response
Genes Dev.
16
452-466
2002
Cricetulus griseus
Manually annotated by BRENDA team
Gu, Y.; Lee, W.; Shen, J.
Site-2 protease responds to oxidative stress and regulates oxidative injury in mammalian cells
Sci. Rep.
4
6268
2014
Cricetulus griseus
Manually annotated by BRENDA team