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Information on EC 3.4.24.79 - pappalysin-1 and Organism(s) Homo sapiens and UniProt Accession Q13219

for references in articles please use BRENDA:EC3.4.24.79
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.79 pappalysin-1
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This record set is specific for:
Homo sapiens
UNIPROT: Q13219 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Cleavage of the Met135-/-Lys bond in insulin-like growth factor binding protein (IGFBP)-4, and the Ser143-/-Lys bond in IGFBP-5
Synonyms
papp-a, pregnancy-associated plasma protein-a, pregnancy-associated plasma protein a, papp-a2, pappa, igfbp-4 protease, pregnancy associated plasma protein-a, pregnancy-associated plasma protein-a2, pappalysin-1, igf binding protein-4 protease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IGFBP-4 protease
-
PAPP-A
PAPP-A2
isoform
pappalysin-1
-
pregnancy associated plasma protein-A
-
pregnancy-associated plasma protein A
-
pregnancy-associated plasma protein-A
-
IGF binding protein-4 protease
-
-
-
-
IGF binding protein-4 specific proteinase
-
-
-
-
IGF-BP-4 proteinase
-
-
-
-
IGFBP proteinase
-
-
IGFBP-4 protease
-
-
-
-
insulin-like growth factor binding protein-4 protease
-
-
-
-
insulin-like growth factor-binding protein proteinase
-
-
PAPP-A
PAPP-A2
-
-
pregnancy-associated plasma protein A
-
-
pregnancy-associated plasma protein-A
pregnancy-associated plasma protein-A2
-
-
pregnancy-related serine protease
-
-
-
-
protein IGF-BP 4 proteinase
-
-
-
-
additional information
-
PAPP-A and PAPP-A2 are unique members in the group of laminin G proteins, the pappalysins represent examples where LG modules are associated with proteinases
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of the Met135-/-Lys bond in insulin-like growth factor binding protein (IGFBP)-4, and the Ser143-/-Lys bond in IGFBP-5
show the reaction diagram
a 400-kDa disulfide-linked dimer that circulates in human pregnancy mainly as a complex with the proform of eosinophil major basic protein, which acts as an inhibitor of the peptidase
Cleavage of the Met135-/-Lys bond in insulin-like growth factor binding protein (IGFBP)-4, and the Ser143-/-Lys bond in IGFBP-5
show the reaction diagram
CAS REGISTRY NUMBER
COMMENTARY hide
151662-33-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inhibitory insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein-2 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
insulin-like growth factor binding protein-5 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-2 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-4 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-5 + H2O
?
show the reaction diagram
-
-
-
?
IGF-binding protein-2 + H2O
?
show the reaction diagram
IGF-binding protein-4 + H2O
?
show the reaction diagram
IGF-binding protein-5 + H2O
?
show the reaction diagram
IGFBP-4 + H2O
?
show the reaction diagram
-
-
-
-
?
IGFBP-5 + H2O
?
show the reaction diagram
-
-
-
-
?
insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
insulin-like growth factor binding protein-5 + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inhibitory insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein-2 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
insulin-like growth factor binding protein-5 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-2 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-4 + H2O
?
show the reaction diagram
-
-
-
?
insulin-like growth factor-binding protein-5 + H2O
?
show the reaction diagram
-
-
-
?
IGF-binding protein-2 + H2O
?
show the reaction diagram
-
-
-
-
?
IGF-binding protein-4 + H2O
?
show the reaction diagram
IGF-binding protein-5 + H2O
?
show the reaction diagram
insulin-like growth factor binding protein-4 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
1 mM used in assay conditions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-phorbol-12,13-didecanoate
100 nM, time-dependent inhibition, possibly through induction of pro-eosinophil major basic protein
stanniocalcin 1
-
-
stanniocalcin 2
-
-
stanniocalcin-1
-
-
stanniocalcin-2
-
beta-mercaptoethanol
-
-
EDTA
-
5 mM
EGTA
-
5 mM
IGF-I
-
the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview
-
IGF-II
-
the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, overview
-
PAC1
-
specific monoclonal antibody inhibiting PAPP-A activity against IGFBP-4, only slight inhibition of IGFBP-5 proteolysis
-
pro-eosinophil major basic protein
-
physiological inhibitor
-
proform of eosinophil major basic protein
-
additional information
-
monoclonal phage-derived scFv antibodies selectively inhibit the cleavage of IGFBP-4 by PAPP-A, none of the antibodies inhibit the homologous proteinase PAPP-A2, which allowed mapping of antibody binding by means of chimeras between PAPP-A and PAPP-A2 to the C-terminal Lin12-Notch repeat module, separated from the proteolytic domain by almost 1000 amino acids, the LNR module requires bound calcium for antibody binding, selective inhibition of IGFBP-4 cleavage by interference with exosite binding, binding site mapping, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
IGF
-
IGF-I
-
the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview
-
IGF-II
-
the rate of IGFBP-4 proteolysis is dramatically increased upon addition of IGF-I or -II with IGF-II being more potent than IGF-I, while IGFBP-5 cleavage is slightly inhibited, the mechanism of IGF-modulated proteolysis of IGFBP-4 and -5 involves changes in both the recognition of substrate and the turnover rate, overview
-
additional information
-
the enzyme expression in fibroblasts is stimulated by pro-inflammatory cytokines involved in repair responses, especially TNFalpha and interleukin 1-beta, the stimulation is inhibited by resveratrol
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00045 - 0.00163
IGFBP-4
-
0.00031 - 0.00064
IGFBP-5
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.64 - 0.89
IGFBP-4
-
0.31 - 0.75
IGFBP-5
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000000068
stanniocalcin-1
isoform PAPP-A, at pH 7.5 and 37°C
-
0.0000012
PAC1 antibody
-
pH 7.5, 37°C, inhibition of PAPP-A
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
assay at
7.5
-
assay at
7.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the enzyme is extracted from plaque tissue samples of endarterectomy and aneurysm patients
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the PAPP-A-proMBP complex is formed at the cell surface in vivo rather than in the circulation, the redox potential of the tissue microenvironment controls the process, chondroitin or dermatan sulfate do not have any effect on complex formation
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PAPP1_HUMAN
1627
0
180973
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
400000
400000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
dimer
-
homodimer, a laminin G-like module is contained within the N-terminal region, secondary structure prediction and sequence alignment, overview
additional information
-
primary sequence, structure motifs, and domain structure and function, the first 350 amino acid residues comprise the proteolytic domain, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
proteolytic modification
-
the enzyme performs autocatalytic cleavage
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E483Q
E483A
-
a proteolytically inactive PAPP-A mutant
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
10 min, no loss of activity
55
-
10 min, no loss of activity
70 - 95
-
10 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
half-life of circulating PAPP-A and proMBP in complex is severalfold higher than both of the uncomplexed proteins
-
high salt condition, SDS, or beta-mercaptoethanol inhibit
-
Triton X-100, Tween-20, NP-40, sodium azide, glycerol do not affect enzyme activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, 1 year, no loss of activity
-
4°C, 3 days, no loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and mutant chimeric PAPP-A and PAPP-A2 from HEK-293T cells
-
recombinant His6-tagged PAPP-A laminin G domain from Escherichia coli by nickel affinity chromatography
-
recombinant PAPP-A wild-type and mutant E483A from HEK-293T cells by immunoaffinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in mouse arterial smooth muscle cells
expressed in SKOV-3 cells
DNA and amino acid sequence determination and anaylsis, sequence comparisons
-
expression of His-tagged wild-type and mutant chimeric PAPP-A and PAPP-A2 in HEK-293T cells
-
expression of PAPP-A wild-type and mutant E483A in HEK-293T cells, co-expression with inhibitor proMBP
-
expression of the enzyme in transgenic mice, expression of FLAG-tagged enzyme in primary mouse osteoblasts
-
expression of the His6-tagged PAPP-A laminin G domain in Escherichia coli, expression of wild-type andtruncation mutant enzymes, comparising residues 1-310, in HEK293T cells
-
overexpression in transgenic mice heart, brain, liver, kidney, forearm, and spleen, expression analysis, overview
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after intravenous unfractionated heparin and low molecular weight heparin free PAPP-A increases significantly (85 to 310fold from baseline)
enzyme blood plasma levels are elevated in patients with acute coronary syndrome
enzyme concentrations are reduced in the first trimester of pregnancies affected by fetal Down's syndrome. interferon-gamma decreases enzyme expression in human fibroblasts
enzyme expression in cultured human dermal fibroblasts, human coronary artery endothelial, osteoblasts, and smooth muscle cells is stimulated by tumor necrosis factor-alpha, interleukin-1beta, interleukin-4, and transforming growth factor-beta. Enzyme expression is upregulated in response to acute injury. Gene expression is induced by forskolin and prostaglandin E2 in human osteoblasts, by bone morphogenic protein-2 in human mesenchymal stem cells, and by follicle-stimulating hormone in bovine granulosa cells in culture
injection of heparin (5000 IU) elicits increase in enzyme concentrations
the concentration of enzyme antigen in follicular fluid increases upon stimulation of ovulation with human chorionic gonadotrophin, but at the same time the enzyme activity is decreased
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
microdialysis against water reverses high salt inhibition
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
pregnancy-associated plasma protein A is a cardiac risk marker
diagnostics
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lawrence, J.B.; Oxvig, C.; Overgaard, M.T.; Sottrup-Jensen, L.; Gleich, G.J.; Hays L.G.; Yates, J.R.3rd; Conover, C.A.
The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by human fibroblasts is pregnancy-associated plasma protein-A
Proc. Natl. Acad. Sci. USA
96
3149-3153
1999
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Chen, B.K.; Overgaard, M.T.; Bale, L.K.; Resch, Z.T.; Christiansen, M.; Oxvig, C.; Conover, C.A.
Molecular regulation of the IGF-binding protein-4 protease system in human fibroblasts: identification of a novel inducible inhibitor
Endocrinology
143
1199-1205
2002
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Lawrence, J.B.; Bale, L.K.; Haddad, T.C.; Clarkson, J.T.; Conover, C.A.
Characterization and partial purification of the insulin-like growth factor (IGF)-dependent IGF binding protein-4-specific protease from human fibroblast conditioned media
Growth Horm. IGF Res.
9
25-34
1999
Homo sapiens
Manually annotated by BRENDA team
Chelius, D.; Conover, C.A.; Baldwin, M.A.; Spencer, E.M.
Characterization of the enzymatic specificity of the IGF-dependent insulin-like growth factor binding protein-4 (IGFBP-4) protease
Growth Horm. IGF Res.
10
360-366
2000
Homo sapiens
Manually annotated by BRENDA team
Laursen, L.S.; Overgaard, M.T.; Soe, R.; Boldt, H.B.; Sottrup-Jensen, L.; Giudice, L.C.; Conover, C.A.; Oxvig, C.
Pregnancy-associated plasma protein-A (PAPP-A) cleaves insulin-like growth factor binding protein (IGFBP)-5 independent of IGF: implications for the mechanism of IGFBP-4 proteolysis by PAPP-A
FEBS Lett.
504
36-40
2001
Homo sapiens
Manually annotated by BRENDA team
Conover, C.A.; Durham, S.K.; Zapf, J.; Masiarz, F.R.; Kiefer, M.C.
Cleavage analysis of insulin-like growth factor (IGF)-dependent IGF-binding protein-4 proteolysis and expression of protease-resistant IGF-binding protein-4 mutants
J. Biol. Chem.
270
4395-4400
1995
Homo sapiens
Manually annotated by BRENDA team
Qin, X.; Byun, D.; Lau, K.H.W.; Baylink, D.J.; Mohan, S.
Evidence that the interaction between insulin-like growth factor (IGF)-II and IGF binding protein (IGFBP)-4 is essential for the action of the IGF-II-dependent IGFBP-4 protease
Arch. Biochem. Biophys.
379
209-216
2000
Homo sapiens
Manually annotated by BRENDA team
Laursen, L.S.; Overgaard, M.T.; Weyer, K.; Boldt, H.B.; Ebbesen, P.; Christiansen, M.; Sottrup-Jensen, L.; Giudice, L.C.; Oxvig, C.
Cell surface targeting of pregnancy-associated plasma protein A proteolytic activity
J. Biol. Chem.
277
47225-47234
2002
Homo sapiens
Manually annotated by BRENDA team
Sun, I.Y.C.; Overgaard, M.T.; Oxvig, C.; Giudice, L.C.
Pregnancy-associated plasma protein A proteolytic activity is associated with the human placental trophoblast cell membrane
J. Clin. Endocrinol. Metab.
87
5235-5240
2002
Homo sapiens
Manually annotated by BRENDA team
Giudice, L.C.; Conover, C.A.; Bale, L.; Faessen, G.H.; Ilg, K.; Sun, I.; Imani, B.; Suen, L.F.; Irwin, J.C.; Christiansen, M.; Overgaard, M.T.; Oxvig, C.
Identification and regulation of the IGFBP-4 protease and its physiological inhibitor in human trophoblasts and endometrial stroma: Evidence for paracrine regulation of IGF-II bioavailability in the placental bed during human implantation
J. Clin. Endocrinol. Metab.
87
2359-2366
2002
Homo sapiens
Manually annotated by BRENDA team
Byun, D.; Mohan, S.; Yoo, M.; Sexton, C.; Baylink, D.J.; Qin, X.
Pregnancy-associated plasma protein-A accounts for the insulin-like growth factor (IGF)-binding protein-4 (IGFBP-4) proteolytic activity in human pregnancy serum and enhances the mitogenic activity of IGF by degrading IGFBP-4 in vitro
J. Clin. Endocrinol. Metab.
86
847-854
2001
Homo sapiens
Manually annotated by BRENDA team
Conover, C.A.; Harrington, S.C.; Bale, L.K.; Oxvig, C.
Surface association of pregnancy-associated plasma protein-A accounts for its colocalization with activated macrophages
Am. J. Physiol. Heart Circ. Physiol.
292
H994-H1000
2007
Homo sapiens
Manually annotated by BRENDA team
Gyrup, C.; Oxvig, C.
Quantitative analysis of insulin-like growth factor-modulated proteolysis of insulin-like growth factor binding protein-4 and -5 by pregnancy-associated plasma protein-A
Biochemistry
46
1972-1980
2007
Homo sapiens
Manually annotated by BRENDA team
Iversen, K.K.; Teisner, A.S.; Teisner, B.; Kliem, A.; Bay, M.; Kirk, V.; Nielsen, H.; Boesgaard, S.; Grande, P.; Clemmensen, P.
Pregnancy-associated plasma protein A in non-cardiac conditions
Clin. Biochem.
41
548-553
2008
Homo sapiens
Manually annotated by BRENDA team
Mueller, T.; Dieplinger, B.; Poelz, W.; Haltmayer, M.
Increased pregnancy-associated plasma protein-A as a marker for peripheral atherosclerosis: results from the Linz Peripheral Arterial Disease Study
Clin. Chem.
52
1096-1103
2006
Homo sapiens
Manually annotated by BRENDA team
Consuegra-Sanchez, L.; Petrovic, I.; Cosin-Sales, J.; Holt, D.W.; Christiansen, M.; Kaski, J.C.
Prognostic value of circulating pregnancy-associated plasma protein-A (PAPP-A) and proform of eosinophil major basic protein (pro-MBP) levels in patients with chronic stable angina pectoris
Clin. Chim. Acta
391
18-23
2008
Homo sapiens
Manually annotated by BRENDA team
Qin, X.; Wergedal, J.E.; Rehage, M.; Tran, K.; Newton, J.; Lam, P.; Baylink, D.J.; Mohan, S.
Pregnancy-associated plasma protein-A increases osteoblast proliferation in vitro and bone formation in vivo
Endocrinology
147
5653-5661
2006
Homo sapiens
Manually annotated by BRENDA team
Rehage, M.; Mohan, S.; Wergedal, J.E.; Bonafede, B.; Tran, K.; Hou, D.; Phang, D.; Kumar, A.; Qin, X.
Transgenic overexpression of pregnancy-associated plasma protein-A increases the somatic growth and skeletal muscle mass in mice
Endocrinology
148
6176-6185
2007
Homo sapiens
Manually annotated by BRENDA team
Boldt, H.B.; Conover, C.A.
Pregnancy-associated plasma protein-A (PAPP-A): a local regulator of IGF bioavailability through cleavage of IGFBPs
Growth Horm. IGF Res.
17
10-18
2007
Danio rerio, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Takifugu rubripes, Xenopus laevis, Tetraodon nigroviridis
Manually annotated by BRENDA team
Glerup, S.; Kloeverpris, S.; Laursen, L.S.; Dagnaes-Hansen, F.; Thiel, S.; Conover, C.A.; Oxvig, C.
Cell surface detachment of pregnancy-associated plasma protein-A requires the formation of intermolecular proteinase-inhibitor disulfide bonds and glycosaminoglycan covalently bound to the inhibitor
J. Biol. Chem.
282
1769-1778
2007
Homo sapiens
Manually annotated by BRENDA team
Mikkelsen, J.H.; Gyrup, C.; Kristensen, P.; Overgaard, M.T.; Poulsen, C.B.; Laursen, L.S.; Oxvig, C.
Selective inhibition of the proteolytic activity of pregnancy-associated plasma protein-A by targeting substrate exosite binding
J. Biol. Chem.
283
16772-16780
2008
Homo sapiens
Manually annotated by BRENDA team
Hajek, P.; Macek, M.; Hladikova, M.; Houbova, B.; Alan, D.; Durdil, V.; Fiedler, J.; Maly, M.; Ostadal, P.; Veselka, J.; Krebsova, A.
Pregnancy-associated plasma protein A and proform eosinophilic major basic protein in the detection of different types of coronary artery disease
Physiol. Res.
57
23-32
2008
Homo sapiens
Manually annotated by BRENDA team
Boldt, H.B.; Glerup, S.; Overgaard, M.T.; Sottrup-Jensen, L.; Oxvig, C.
Definition, expression, and characterization of a protein domain in the N-terminus of pregnancy-associated plasma protein-A distantly related to the family of laminin G-like modules
Protein Expr. Purif.
48
261-273
2006
Homo sapiens
Manually annotated by BRENDA team
Prinzen, C.; Truembach, D.; Wurst, W.; Endres, K.; Postina, R.; Fahrenholz, F.
Differential gene expression in ADAM10 and mutant ADAM10 transgenic mice
BMC Genomics
10
66
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Joaquin, C.; Aguilera, E.; Granada, M.L.; Pastor, M.C.; Salinas, I.; Alonso, N.; Sanmarti, A.
Effects of GH treatment in GH-deficient adults on adiponectin, leptin and pregnancy-associated plasma protein-A
Eur. J. Endocrinol.
158
483-490
2008
Homo sapiens
Manually annotated by BRENDA team
Pellitero, S.; Reverter, J.; Granada, M.L.; Pizarro, E.; Pastor, M.C.; Tassies, D.; Reverter, J.C.; Salinas, I.; Sanmarti, A.
Association of the insulin-growth factor-1 (IGF-1)/pregnancy-associated plasma protein-A (PAPP-A) system and adipocytokine levels with the presence and the morphology of carotid plaques in type 2 diabetes mellitus patients with stable glycemic control
Eur. J. Endocrinol.
160
925-932
2009
Homo sapiens
Manually annotated by BRENDA team
Kagan, K.O.; Wright, D.; Valencia, C.; Maiz, N.; Nicolaides, K.H.
Screening for trisomies 21, 18 and 13 by maternal age, fetal nuchal translucency, fetal heart rate, free beta-hCG and pregnancy-associated plasma protein-A
Hum. Reprod.
23
1968-1975
2008
Homo sapiens
Manually annotated by BRENDA team
Amor, D.J.; Xu, J.X.; Halliday, J.L.; Francis, I.; Healy, D.L.; Breheny, S.; Baker, H.W.; Jaques, A.M.
Pregnancies conceived using assisted reproductive technologies (ART) have low levels of pregnancy-associated plasma protein-A (PAPP-A) leading to a high rate of false-positive results in first trimester screening for Down syndrome
Hum. Reprod.
24
1330-1338
2009
Homo sapiens
Manually annotated by BRENDA team
Bestwick, J.P.; Huttly, W.J.; Wald, N.J.
First trimester Downs syndrome screening marker values and cigarette smoking: new data and a meta-analysis on free beta human chorionic gonadotophin, pregnancy-associated plasma protein-A and nuchal translucency
J. Med. Screen.
15
204-206
2008
Homo sapiens
Manually annotated by BRENDA team
Deveci, K.; Sogut, E.; Evliyaoglu, O.; Duras, N.
Pregnancy-associated plasma protein-A and C-reactive protein levels in pre-eclamptic and normotensive pregnant women at third trimester
J. Obstet. Gynaecol. Res.
35
94-98
2009
Homo sapiens
Manually annotated by BRENDA team
Nishizawa, H.; Pryor-Koishi, K.; Suzuki, M.; Kato, T.; Kogo, H.; Sekiya, T.; Kurahashi, H.; Udagawa, Y.
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients
Mol. Hum. Reprod.
14
595-602
2008
Homo sapiens
Manually annotated by BRENDA team
Pihl, K.; Larsen, T.; Krebs, L.; Christiansen, M.
First trimester maternal serum PAPP-A, beta-hCG and ADAM12 in prediction of small-for-gestational-age fetuses
Prenat. Diagn.
28
1131-1135
2008
Homo sapiens
Manually annotated by BRENDA team
Kagan, K.O.; Wright, D.; Spencer, K.; Molina, F.S.; Nicolaides, K.H.
First-trimester screening for trisomy 21 by free beta-human chorionic gonadotropin and pregnancy-associated plasma protein-A: impact of maternal and pregnancy characteristics
Ultrasound Obstet. Gynecol.
31
493-502
2008
Homo sapiens
Manually annotated by BRENDA team
Kagan, K.O.; Wright, D.; Baker, A.; Sahota, D.; Nicolaides, K.H.
Screening for trisomy 21 by maternal age, fetal nuchal translucency thickness, free beta-human chorionic gonadotropin and pregnancy-associated plasma protein-A
Ultrasound Obstet. Gynecol.
31
618-624
2008
Homo sapiens
Manually annotated by BRENDA team
Conover, C.A.; Mason, M.A.; Bale, L.K.; Harrington, S.C.; Nyegaard, M.; Oxvig, C.; Overgaard, M.T.
Transgenic overexpression of pregnancy-associated plasma protein-A in murine arterial smooth muscle accelerates atherosclerotic lesion development
Am. J. Physiol. Heart Circ. Physiol.
299
H284-H291
2010
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Wittfooth, S.; Tertti, R.; Lepaentalo, M.; Porela, P.; Qin, Q.P.; Tynjaelae, J.; Inkinen, O.; Perttilae, J.; Airaksinen, K.E.; Pettersson, K.
Studies on the effects of heparin products on pregnancy-associated plasma protein A
Clin. Chim. Acta
412
376-381
2011
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Boldt, H.B.; Conover, C.A.
Overexpression of pregnancy-associated plasma protein-A in ovarian cancer cells promotes tumor growth in vivo
Endocrinology
152
1470-1478
2011
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Gaidamauskas, E.; Gyrup, C.; Boldt, H.; Schack, V.; Overgaard, M.; Laursen, L.; Oxvig, C.
IGF dependent modulation of IGF binding protein (IGFBP) proteolysis by pregnancy-associated plasma protein-A (PAPP-A): Multiple PAPP-A-IGFBP interaction sites
Biochim. Biophys. Acta
1830
2701-2709
2013
Homo sapiens (Q13219)
Manually annotated by BRENDA team
Jespersen, C.; Vestergaard, K.; Schou, M.; Teisner, B.; Iversen, K.
The effect of heparin on pregnancy associated plasma protein-A concentration in healthy, non-pregnant individuals
Clin. Biochem.
48
75
2015
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Delia, P.; Marzioni, D.; Castellucci, M.; Mocci, C.; Pala, A.
Homodimeric pregnancy-associated plasma protein-A in normal human placenta of first and third trimester of pregnancy: Biochemical and morphological observations
Placenta
33
942-945
2012
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Pan, H.; Hanada, S.; Zhao, J.; Mao, L.; Ma, M.
Protein secretion is required for pregnancy-associated plasma protein-A to promote lung cancer growth in vivo
PLoS ONE
7
e48799
2012
Homo sapiens (Q13219)
Manually annotated by BRENDA team
Conover, C.
Key questions and answers about pregnancy-associated plasma protein-A
Trends Endocrinol. Metab.
23
242-249
2012
Homo sapiens (Q13219)
Manually annotated by BRENDA team
Jepsen, M.R.; Kloverpris, S.; Botkjaer, J.A.; Wissing, M.L.; Andersen, C.Y.; Oxvig, C.
The proteolytic activity of pregnancy-associated plasma protein-A is potentially regulated by stanniocalcin-1 and -2 during human ovarian follicle development
Hum. Reprod.
31
866-874
2016
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Kloverpris, S.; Mikkelsen, J.H.; Pedersen, J.H.; Jepsen, M.R.; Laursen, L.S.; Petersen, S.V.; Oxvig, C.
Stanniocalcin-1 potently inhibits the proteolytic activity of the metalloproteinase pregnancy-associated plasma protein-A
J. Biol. Chem.
290
21915-21924
2015
Homo sapiens (Q13219)
Manually annotated by BRENDA team
Espelund, U.S.; Bjerre, M.; Hjortebjerg, R.; Rasmussen, T.R.; Lundby, A.; Hoeflich, A.; Folkersen, B.H.; Oxvig, C.; Frystyk, J.
Insulin-like growth factor bioactivity, stanniocalcin-2, pregnancy-associated plasma protein-A, and IGF-binding protein-4 in pleural fluid and serum from patients with pulmonary disease
J. Clin. Endocrinol. Metab.
102
3526-3534
2017
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team
Cirillo, P.; Conte, S.; Pellegrino, G.; Ziviello, F.; Barra, G.; De Palma, R.; Leonardi, A.; Trimarco, B.
Pregnancy-associated plasma protein-A promotes TF procoagulant activity in human endothelial cells by Akt-NF-kappaB axis
J. Thromb. Thrombolysis
42
225-232
2016
Homo sapiens (Q13219), Homo sapiens
Manually annotated by BRENDA team