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IUBMB Comments A 22.5 kDa endopeptidase from the venom of the jararaca snake (Bothrops jararaca ), insensitive to phosphoramidon at 0.5 mM. In peptidase family M12 (astacin family)
Reaction Schemes
Cleavage of Gln4-/-His, Ser9-/-His and Ala14-/-Leu of insulin B chain and Pro-/-Phe of angiotensin I
Synonyms
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Bothrops metalloendopeptidase J
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J Protease
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Cleavage of Gln4-/-His, Ser9-/-His and Ala14-/-Leu of insulin B chain and Pro-/-Phe of angiotensin I
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hydrolysis of peptide bond
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angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
casein + H2O
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Substrates: -
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insulin B chain + H2O
FVNQ + HLCGS + HLVEA + LYLVCGERGFFYTPKA
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Substrates: -
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Insulin B-chain + H2O
Hydrolyzed insulin B-chain
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Substrates: cleavage of Gln4-His, Ser9-His and Ala14-Leu
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angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
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Substrates: cleavage of Pro-Phe
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angiotensin I + H2O
Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
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Substrates: -
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Ca2+
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activates
Ca2+
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increases reaction velocity, half-maximum activation at 0.4 mM
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5,5'-dithiobis(2-nitrobenzoic acid)
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1 mM: 70% inhibition, in presence of 2 mM Ca2+: 30% inhibition
DM43
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serum from opossum, inhibits the fibrinogenolytic activity of bothrolysin and forms a stable complex with it
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DTNB
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inhibitory, partially prevented by addition of Ca2+
Protein inhibitor
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from blood plasma of Bothrops jararaca, completely inhibits
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1,10-phenanthroline
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+ 4 mM Ca2+: 85% inhibition
1,10-phenanthroline
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totally inhibits at 0.1 mM
EDTA
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0.1 mM: complete inhibition
EDTA
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totally inhibits at 0.1 mM
additional information
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phosphoramidon, leupeptin, epoxysuccinyl derivatives e.g. E-64, Ep-475 or Ep-479, all 0.5 mM, are no inhibitors
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additional information
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not inhibited by phosphoramidon at 0.5 mM and E-64 and its derivates
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brenda
jararaca snake
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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VMXBN_BOTJA
29
0
3433
Swiss-Prot
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23000
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23000
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1 * 23000, Bothrops jararaca, SDS-PAGE
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monomer
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1 * 23000, Bothrops jararaca, SDS-PAGE
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by column chromatography
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Tanizaki, M.M.; Zingali, R.B.; Kawazaki, H.; Imajoh, S.; Yamazaki, S.; Suzuki, K.
Purification and some characteristics of a zinc metalloprotease from the venom of Bothrops jararaca (jararaca)
Toxicon
27
747-755
1989
Bothrops jararaca
brenda
Tanizaki, M.M.
Bothrolysin
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
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657-658
2004
Bothrops jararaca
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brenda
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