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3.4.24.48
venom
bothrops
jararaca
metalloproteinase
svmps
autolysis
cysteine-rich
frederick
disintegrins
disintegrin-like
coagulation
envenoming
medicine
The expected taxonomic range for this enzyme is: Crotalus ruber ruber
Reaction Schemes
Cleavage of His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in the B chain of insulin; His-/-Pro, Pro-/-Phe, and Trp-/-Ser of angiotensin I; and Gly-/-Phe of Met enkephalin
Synonyms
Crotalus ruber metalloendopeptidase II, Hemorrhagic toxin II, HT-2, red rattlesnake metalloendopeptidase,
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Crotalus ruber metalloendopeptidase II
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red rattlesnake metalloendopeptidase
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Hemorrhagic toxin II
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HT-2
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Cleavage of His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in the B chain of insulin; His-/-Pro, Pro-/-Phe, and Trp-/-Ser of angiotensin I; and Gly-/-Phe of Met enkephalin
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hydrolysis of peptide bond
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(Met)enkephalin + H2O
?
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cleavage at Gly3-Phe4
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-
?
angiotensin + H2O
?
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cleavage at Pro7-Phe8 and His6-Pro7
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-
?
angiotensin I + H2O
?
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i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
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-
?
Dimethylcasein + H2O
?
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?
hide powder azure + H2O
?
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-
?
luteinizing hormone releasing hormone + H2O
?
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cleavage at Gly6-Leu7 and Trp3-Ser4
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?
Luteinizing hormone-releasing hormone + H2O
?
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cleavage sites: Trp3-Ser4, Gly6-Leu7
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-
?
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
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i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
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?
oxidized insulin B chain + H2O
?
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?
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
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cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24
-
?
additional information
?
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exhibits proteolytic, hemorrhatic and myonecrotic activity
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?
Fibrinogen + H2O
?
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Aalpha chain, not beta or gamma chain
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?
Fibrinogen + H2O
?
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hydrolysis of Aalpha and Bbeta chains, whereas the gamma chain is resistant to hydrolysis
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?
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Na+
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metalloproteinase, contains 10.3 mol Na+/mol enzyme
additional information
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less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme
Ca2+
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metalloproteinase, contains 1.1 mol Ca2+/mol enzyme
Ca2+
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1.1 mol of calcium per mol of protein
Zn2+
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metalloproteinase, contains 1.1 mol Zn2+/mol enzyme
Zn2+
-
1.1 mol of zinc per mol of protein
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additional information
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no inhibition by benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB
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additional information
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not inhibited by benzamidine
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additional information
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pI: 5.2
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brenda
red rattlesnake
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brenda
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brenda
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VM1H2_CRORU
202
0
23321
Swiss-Prot
other Location (Reliability: 3 )
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24000
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x * 24000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
25000
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25000
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x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
additional information
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amino acid sequence (81% identity of toxin HT-2 and Crotalus atrox toxin Ht-d)
additional information
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amino acid composition of isozyme HT-2
additional information
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amino acid composition of isozyme HT-2
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?
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x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
?
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x * 24000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
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by gel filtration and ion-exchange chromatography
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from lyophilized crude venom
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medicine
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HT-2 has hemorrhagic and lethal activity
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Takeya, H.; Onikura, A.; Nikai, T.; Sugihara, H.; Iwanaga, S.
Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from the venom of Crotalus ruber ruber
J. Biochem.
108
711-719
1990
Crotalus ruber ruber
brenda
Mori, N.; Nikali, T.; Sugihara, H.; Tu, A.T.
Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom
Arch. Biochem. Biophys.
253
108-121
1987
Crotalus ruber ruber
brenda
Tu, A.T.
Ruberlysin
Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
682-683
2004
Crotalus ruber ruber
-
brenda
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