Information on EC 3.4.24.48 - ruberlysin

for references in articles please use BRENDA:EC3.4.24.48
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The expected taxonomic range for this enzyme is: Crotalus ruber ruber

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EC NUMBER
COMMENTARY hide
3.4.24.48
-
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RECOMMENDED NAME
GeneOntology No.
ruberlysin
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Cleavage of His10-/-Leu, Ala14-/-Leu, Tyr16-/-Leu and Gly23-/-Phe bonds in the B chain of insulin; His-/-Pro, Pro-/-Phe, and Trp-/-Ser of angiotensin I; and Gly-/-Phe of Met enkephalin
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
846020-01-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Met)enkephalin + H2O
?
show the reaction diagram
-
cleavage at Gly3-Phe4
-
-
?
Angiotensin + H2O
?
show the reaction diagram
-
cleavage at Pro7-Phe8 and His6-Pro7
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe
-
-
-
casein + H2O
?
show the reaction diagram
Dimethylcasein + H2O
?
show the reaction diagram
Fibrin + H2O
?
show the reaction diagram
-
-
-
-
-
Fibrinogen + H2O
?
show the reaction diagram
hide powder azure + H2O
?
show the reaction diagram
luteinizing hormone releasing hormone + H2O
?
show the reaction diagram
-
cleavage at Gly6-Leu7 and Trp3-Ser4
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
cleavage sites: Trp3-Ser4, Gly6-Leu7
-
-
-
Met-enkephalin + H2O
Tyr-Gly-Gly + Phe-Met
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4
-
-
oxidized insulin B chain + H2O
?
show the reaction diagram
-
-
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24
-
-
additional information
?
-
-
exhibits proteolytic, hemorrhatic and myonecrotic activity
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
-
metalloproteinase, contains 10.3 mol Na+/mol enzyme
additional information
-
less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
additional information
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 5.2
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
x * 24000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE
additional information
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration and ion-exchange chromatography
-
from lyophilized crude venom
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
HT-2 has hemorrhagic and lethal activity
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LINKS TO OTHER DATABASES (specific for EC-Number 3.4.24.48)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)