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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Cleavage of Pro-/-Phe and Ala-/-Ala bonds
Synonyms
proteinase yscd, protease d, saccharolysin,
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Oligopeptidase YSCD
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Proteinase, Saccharomyces cerevisiae, yscD
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EC 3.4.22.22
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formerly
PrD
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protease D
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Cleavage of Pro-/-Phe and Ala-/-Ala bonds
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hydrolysis of peptide bond
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Ac-Ala-Ala-Pro-Met-NHPhNO2 + H2O
?
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?
Ac-Ala-Ala-Pro-Phe-NHPhNO2 + H2O
?
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-
-
?
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide + H2O
Acetyl-Ala + Ala-Pro-Ala 4-nitroanilide
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-
-
?
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
Acetyl-Ala + Ala-Pro-Phe 4-nitroanilide
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-
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?
acetyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
?
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-
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?
Benzoyl-Pro-Phe-Arg 4-nitroanilide + H2O
Benzoyl-Pro + Phe-Arg 4-nitroanilide
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?
bradykinin + H2O
?
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cleaved at the Phe-Ser bond
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?
Bz-Pro-Phe-Arg-NHPhNO2 + H2O
?
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-
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?
Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
?
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?
MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 + H2O
?
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-
?
Methoxysuccinyl-Ala-Ala-Pro-Met 4-nitroanilide + H2O
Methoxysuccinyl-Ala + Ala-Pro-Met 4-nitroanilide
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?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
?
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?
Pz-Pro-Leu-Gly-Pro-D-Arg,Mcc-Pro-Leu-Gly-Pro-D-Lys(Dnp) + H2O
?
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?
additional information
?
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N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
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?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
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?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
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?
additional information
?
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not: methylcasein
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?
additional information
?
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enzyme is not involved in any vital event in mitochondrial biogenesis
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?
additional information
?
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no cleavage of peptides with a free N-terminus, azocasein and (3H)methylcasein
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?
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additional information
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enzyme is not involved in any vital event in mitochondrial biogenesis
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?
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additional information
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metalloprotease, not affeted by Cu2+, Co2+, Mn2+, and Ca2+
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1,10-phenanthroline
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complete inhibition at 0.1 mM
2-mercaptoethanol
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19% inhibition at 5 mM
3,4-dichloroisocoumarin
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71% inhibition at 1 mM
4-chloromercuribenzoate
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complete inhibition at 0.1 mM
4-hydroxymercuribenzoate
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100% inhibition with 0.1 mM
Cd2+
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33% inhibition at 0.5 mM
Cpp-Ala-Ala-Phe-p-aminobenzoic acid
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strongly inhibits
DTT
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19% inhibition at 1 mM
EGTA
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35% inhibition at 5 mM
Hg2+
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complete inhibition at 0.05 mM
Mg2+
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21% inhibition at 0.5 mM
pepstatin A
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15% inhibition at 1 mM
PMSF
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50% inhibition at 1 mM
Zn2+
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73% inhibition at 0.5 mM
EDTA
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EDTA
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inhibits 50% at 1 mM and 90% at 10 mM, can be efficiently reactivated by the addition of Zn2+, Co2+ and Mn2+
HgCl2
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HgCl2
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100% inhibition with 0.01 mM
additional information
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no inhibition by EDTA at 5 mM
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additional information
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not inhibited by DTT, N-ethylmaleimide, iodoacetic acid or iodoacetamide
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iodoacetate
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11% activation at 1 mM
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0.19
Ac-Ala-Ala-Pro-Met-NHPhNO2
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0.085
Ac-Ala-Ala-Pro-Phe-NHPhNO2
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0.19
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
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0.085
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide
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0.06
benzoyl-Pro-Phe-Arg 4-nitroanilide
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0.06
Bz-Pro-Phe-Arg-NHPhNO2
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0.029
Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys
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0.035
MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
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0.035
Methoxysuccinyl-Ala-Ala-Pro-Met 4-nitroanilide
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0.006
Pz-Pro-Leu-Gly-Pro-D-Arg
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-
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6000
Acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
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38000
Acetyl-Ala-Ala-Pro-Phe 4-nitroanilide
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1500
benzoyl-Pro-Phe-Arg 4-nitroanilide
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38000
Methoxysuccinyl-Ala-Ala-Pro-Met 4-nitroanilide
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-
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additional information
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5.5
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methoxy-succinyl-Ala-Ala-Pro-Met 4-nitroanilide
5.8 - 6
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acetyl-Ala-Ala-Pro-Ala 4-nitroanilide
6.5 - 7
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benzoyl-Pro-Phe-Arg 4-nitroanilide
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5.5
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with MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
5.9
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with Ac-Ala-Ala-Pro-Met-NHPhNO2
7.9
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with Bz-Pro-Phe-Arg-NHPhNO2
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isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
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brenda
isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
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brenda
Western corn rootworm
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brenda
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brenda
mutants devoid of proteinase yscD
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brenda
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midgut juice (pH 5.75) from third instars larvae
brenda
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brenda
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intermembrane space
brenda
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PRTD_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
712
0
81934
Swiss-Prot
Mitochondrion (Reliability: 5 )
A3GFY2_PICST
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
687
0
80151
TrEMBL
other Location (Reliability: 2 )
A3GF02_PICST
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
658
0
75446
TrEMBL
other Location (Reliability: 1 )
B0E704_ENTDS
Entamoeba dispar (strain ATCC PRA-260 / SAW760)
218
0
25356
TrEMBL
other Location (Reliability: 2 )
C0RGZ7_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
489
0
53632
TrEMBL
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A0A0F8AY81_CERFI
700
0
79215
TrEMBL
other Location (Reliability: 3 )
B8M3X1_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
716
0
81496
TrEMBL
other Location (Reliability: 2 )
B8MCP2_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
685
0
78511
TrEMBL
other Location (Reliability: 5 )
C0G440_9RHIZ
489
0
53612
TrEMBL
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K0L075_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
802
0
92931
TrEMBL
other Location (Reliability: 3 )
A0A084FZN3_PSEDA
935
1
105003
TrEMBL
Mitochondrion (Reliability: 3 )
C4WI16_9RHIZ
484
0
53066
TrEMBL
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W1QHJ3_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
680
0
79082
TrEMBL
Secretory Pathway (Reliability: 1 )
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65000
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1 * 65000, SDS-PAGE
83000
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1 * 83000, Saccharomyces cerevisiae, SDS-PAGE
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monomer
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1 * 65000, SDS-PAGE
monomer
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1 * 65000, SDS-PAGE
monomer
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1 * 65000, SDS-PAGE
monomer
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1 * 83000, Saccharomyces cerevisiae, SDS-PAGE
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additional information
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deletion of Prd1, in deltayme1 cells increase of large peptides in the mitochondrial supernatant, in delta mop112 cells no effect on growth
additional information
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prd1-6 mutants with thermolabile saccharolysin activity, prd1::URA3 mutants devoid of the cytoplasmic and mitochondrial activities
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250fold by chromatographies
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native enzyme 2560fold by fractionation with protamine sulfate, anion exchange chromatography on a weak followed by a strong anion exchange resin, and gel filtration
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degradation
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most likely major intracellular oligopeptidase responsible for the degradation of peptides resulting from nonvacuolar proteolysis
degradation
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Prd1 together with Mop112 is involved in the complete degradation of a large number of mitochondrial proteins to amino acids and therefore broadly influences the peptide efflux from mitochondria
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Achstetter, T.; Ehmann, C.; Wolf, D.H.
Proteinase yscD. Purification and characterization of a new yeast peptidase
J. Biol. Chem.
260
4585-4590
1985
Saccharomyces cerevisiae
brenda
Garcia-Alvarez, N.; Teichert, U.; Wolf, D.H.
Proteinase yscD mutants of yeast. Isolation and characterization
Eur. J. Biochem.
163
339-346
1987
Saccharomyces cerevisiae
brenda
dEnfert, C.; Ibrahim-Granet, O.
Saccharolysin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
1
360-361
2004
Saccharomyces cerevisiae
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brenda
Kambacheld, M.; Augustin, S.; Tatsuta, T.; Mueller, S.; Langer, T.
Role of the novel metallopeptidase Mop112 and saccharolysin for the complete degradation of proteins residing in different subcompartments of mitochondria
J. Biol. Chem.
280
20132-20139
2005
Saccharomyces cerevisiae
brenda
Bolumar, T.; Sanz, Y.; Aristoy, M.C.; Toldra, F.
Purification and characterisation of proteases A and D from Debaryomyces hansenii
Int. J. Food Microbiol.
124
135-141
2008
Debaryomyces hansenii, Debaryomyces hansenii CECT 12487
brenda
Kaiser-Alexnat, R.
Protease activities in the midgut of Western corn rootworm (Diabrotica virgifera virgifera LeConte)
J. Invertebr. Pathol.
100
169-174
2009
Diabrotica virgifera virgifera
brenda
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3.4.24.37 saccharolysin
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