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Information on EC 3.4.24.36 - leishmanolysin and Organism(s) Leishmania donovani and UniProt Accession P23223

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.36 leishmanolysin
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Select one or more organisms in this record: ?
This record set is specific for:
Leishmania donovani
UNIPROT: P23223 not found.
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Word Map
The taxonomic range for the selected organisms is: Leishmania donovani
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-/-Leu-Lys-Lys-
Synonyms
major surface glycoprotein, cell surface protease, leishmanolysin, major surface protease, surface protease gp63, glycoprotein gp63, promastigote surface protease, metalloprotease gp63, glycoprotein 63, gp63 protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
virulence factor major surface protease
-
Cell surface protease
-
-
-
-
Glycoprotein gp63
GP63 protein
-
-
-
-
Leishmania metalloproteinase
-
-
-
-
Major surface glycoprotein
-
-
-
-
Promastigote surface endopeptidase
-
-
-
-
Surface acid proteinase
-
-
-
-
surface protein GP63
-
-
virulence factor major surface protease
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
161052-06-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
transcription factor AP-1 + H2O
?
show the reaction diagram
-
inactivation and degradation
-
-
?
additional information
?
-
-
role of enzyme in promastigote multiplication connected with its fibronectin-like properties
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
transcription factor AP-1 + H2O
?
show the reaction diagram
-
inactivation and degradation
-
-
?
additional information
?
-
-
role of enzyme in promastigote multiplication connected with its fibronectin-like properties
-
-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
metacyclic promastigote exosomes contain the highest, and logarithmic exosomes have the lowest abundance of total major surface protease GP63. Among the major surface protease classes, major surface protease C class has the greatest variety of isoforms, but is least abundant in all exosomes. All major surface protease classes are present at higher levels in exosomes released from stationary or metacyclic promastigotes than logarithmic promastigotes
-
Manually annotated by BRENDA team
-
GP63 is able to act on its substrate proteins within the nucleus of its host cell
Manually annotated by BRENDA team
metacyclic promastigote exosomes contain the highest, and logarithmic exosomes have the lowest abundance of total major surface protease GP63. Among the major surface protease classes, major surface protease C class has the greatest variety of isoforms, but is least abundant in all exosomes. All major surface protease classes are present at higher levels in exosomes released from stationary or metacyclic promastigotes than logarithmic promastigotes
-
Manually annotated by BRENDA team
-
GP63 is able to act on its substrate proteins within the nucleus of its host cell
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
Leishmania-induced inactivation of the macrophage transcription factor AP-1 is mediated by the parasite metalloprotease GP63 at the cell surface, overview. GP63 but not LPG is highly involved in the mechanism responsible for the inactivation of AP-1 transcription factor. GP63 action requires macrophage lipid raft and is not dependent on parasite phagocytosis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GP63_LEIDO
590
1
62950
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62950
x * 62950, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 62950, calculated
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
homology modeling based on the structure of Leishmania major gp63. The protein consists of the N-terminal, central and C-terminal domain
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme-deficient transfectants due to expression of enzyme antisense mRNA show increased generation time, altered cell morphology, accumulation of cells in G2/M phase of cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth of transfectants can be stimulated by addition of a fusion protein containing the fibronectin-like SRYD motif and the zinc-binding domain of enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged full-length gp63 from Escherichia coli BL21 (DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of full-length gp63 gene in mammalian pcDNA3.1 (2/2) expression vector, expression in CHO cells and of His-tagged protein in Escherichia coli BL21 (DE3) pLysS
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
DNA/DNA, DNA/protein and protein/protein based vaccination using gp63 against Leishmania donovani inducing immune responses and conferred protection against challenge infection, humural responses, quantitative overview
pharmacology
-
cationic distearoyl phosphatidylcholine liposomes, used as vaccine adjuvant with the immunodominant 63 kDa glycoprotein of promastigotes, induce significant protection against progressive visceral leishmaniasis in susceptible BALB/c mice. gp63 used without adjuvant elicits partial protection but in association with liposomes exhibits marked resistance in both the livers and spleens of the mice challenged 10 days after the last vaccination. The protective efficacy of liposomal gp63 vaccination is dose dependent, with 2.5 microg of protein showing optimal protection. Mice challenged 12 weeks after immunization are still protected, and a mixed Th1/Th2 response has been induced following immunization
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pandey, S.; Chakraborti, P.; Sharma, R.; Bandyopadhyay, S.; Sarkar, D.; Adhya, S.
Involvement of Leishmania donovani major surface glycoprotein gp63 in promastigote multiplication
J. Biosci.
29
15-22
2004
Leishmania donovani
Manually annotated by BRENDA team
Razzazan, A.; Saberi, M.R.; Jaafari, M.R.
Insights from the analysis of a predicted model of gp63 in Leishmania donovani
Bioinformation
3
114-118
2008
Leishmania donovani (P23223), Leishmania donovani
Manually annotated by BRENDA team
Bhowmick, S.; Ravindran, R.; Ali, N.
gp63 in stable cationic liposomes confers sustained vaccine immunity to susceptible BALB/c mice infected with Leishmania donovani
Infect. Immun.
76
1003-1015
2008
Leishmania donovani
Manually annotated by BRENDA team
Mazumder, S.; Maji, M.; Das, A.; Ali, N.
Potency, efficacy and durability of DNA/DNA, DNA/protein and protein/protein based vaccination using gp63 against Leishmania donovani in BALB/c mice
PLoS ONE
6
e14644
2011
Leishmania donovani (C7EX18)
Manually annotated by BRENDA team
Contreras, I.; Gomez, M.; Nguyen, O.; Shio, M.; McMaster, R.; Olivier, M.
Leishmania-induced inactivation of the macrophage transcription factor AP-1 is mediated by the parasite metalloprotease GP63
PLoS Pathog.
6
e1001148
2010
Leishmania donovani
Manually annotated by BRENDA team
Marshall, S.; Kelly, P.H.; Singh, B.K.; Pope, R.M.; Kim, P.; Zhanbolat, B.; Wilson, M.E.; Yao, C.
Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes
Parasit. Vectors
11
355
2018
Leishmania amazonensis (Q27673), Leishmania chagasi (P15706), Leishmania donovani (P23223), Leishmania donovani (Q25274), Leishmania donovani (Q967C0), Leishmania donovani (Q967C2), Leishmania donovani donovani (F0V646), Leishmania donovani donovani (F0V651), Leishmania infantum (A4HUF6), Leishmania infantum (A4HUF9), Leishmania infantum (A4HUG0), Leishmania infantum (E9AHH5), Leishmania infantum, Leishmania major (P08148), Leishmania mexicana (E9AN53), Leishmania mexicana (P43150), Leishmania mexicana U1103 (E9AN53), Leishmania tropica (Q8MNZ1)
Manually annotated by BRENDA team