show all | hide all No of entries

Information on EC 3.4.24.35 - gelatinase B and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC3.4.24.35
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.35 gelatinase B
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Cleavage of gelatin types I and V and collagen types IV and V
Synonyms
mmp-9, matrix metalloproteinase-9, matrix metalloproteinase 9, gelatinase b, matrix metallopeptidase 9, matrix metalloprotease-9, mmp 9, 92-kda gelatinase, 92 kda gelatinase, 92-kda type iv collagenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
92 kDa gelatinase
92 kDa type IV collagenase
-
-
92 kDa type IV collagenase proMMP-9
-
-
92-kDa Gelatinase
-
-
-
0
92-kDa gelatinase B
-
-
92-kDa Type IV collagenase
95 kDa type IV collagenase/gelatinase
-
-
-
0
Collagenase IV
-
-
-
0
Collagenase type IV
-
-
-
0
gelatinase
-
gelatinase B
Gelatinase MMP 9
-
-
-
0
GELB
-
-
-
0
Macrophage gelatinase
-
-
-
0
matrix metallopeptidase 9
matrix metallopeptidase 9 gelatinase B
-
-
matrix metalloprotease-9
-
-
Matrix metalloproteinase 9
matrix metalloproteinase-9
metrix metalloproteinase-9
-
-
MMP 9
MMP-9
neutrophil collagenase
-
-
pro-matrix metalloproteinase-9
-
-
progelatinase
-
Type IV collagen metalloproteinase
-
-
-
0
Type IV collagenase
Type IV collagenase/gelatinase
Type V collagenase
-
-
-
0
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
0
CAS REGISTRY NUMBER
COMMENTARY hide
146480-36-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Leu-(3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl)-Ala-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
0
?
(7-methoxycoumaryl-4yl)acetylPLGLA2pr(2,4-dinitrophenol)-AR-NH2 + H2O
?
show the reaction diagram
-
-
?
2,4-dinitrophenyl-Pro-beta-cyclohexylalanyl-Gly-Cys(Me)-His-Ala-Lys(N-Me-2-aminobenzoyl)-NH2 + H2O
?
show the reaction diagram
-
a quenched fluorogenic substrate
-
0
?
2,4-Dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 + H2O
2,4-Dinitrophenyl-Pro-Leu-Gly + Leu-Trp-Ala-D-Arg-NH2
show the reaction diagram
-
and peptide substrates derived from, utility of substrate mapping form examining the subsite specificities and the optimization of proteinase substrates
-
?
65000 MW form of vitronectin + H2O
?
show the reaction diagram
-
-
-
0
?
Ac-L-Pro-L-Pro(OH)-Gly-L-Gln-Gly-L-Ala-L-Thr-Gly-L-Glu-L-Pro(OH)-Gly-NH2 + H2O
?
show the reaction diagram
-
-
-
0
?
actin + H2O
?
show the reaction diagram
-
-
-
0
?
Aggrecan core protein + H2O
?
show the reaction diagram
-
-
-
0
?
alpha-enolase + H2O
?
show the reaction diagram
-
-
-
0
?
annexin I + H2O
?
show the reaction diagram
-
-
-
0
?
Arp2/3 complex subunit + H2O
?
show the reaction diagram
-
-
-
0
?
betaB1 crystallin + H2O
?
show the reaction diagram
-
main enzyme substrate in lens extract
-
0
?
citrate synthase + H2O
?
show the reaction diagram
-
-
-
0
?
Collagen + H2O
?
show the reaction diagram
Collagen type I + H2O
?
show the reaction diagram
Collagen type III + H2O
?
show the reaction diagram
collagen type IV + H2O
?
show the reaction diagram
collagen type V + H2O
?
show the reaction diagram
-
-
-
0
?
collagen type XI + H2O
?
show the reaction diagram
-
-
-
0
?
DNP-Pro-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys(N-Me-Abz)-NH2 + H2O
DNP-Pro-cyclohexyl-Ala-Gly + Cys(Me)-His-Ala-Lys(N-Me-Abz)-NH2
show the reaction diagram
-
-
0
?
Elastin + H2O
?
show the reaction diagram
-
-
-
0
?
ephB1 + H2O
?
show the reaction diagram
-
-
-
?
epidermal growth factor + H2O
?
show the reaction diagram
ezrin + H2O
?
show the reaction diagram
-
-
-
0
?
fibrillar collagen + H2O
?
show the reaction diagram
filamin B + H2O
?
show the reaction diagram
-
-
-
0
?
fragments of human collagen type II + H2O
?
show the reaction diagram
-
fragments obtained by cleavage with collagenases MMP-1, MMP-8, or MMP-13. Enzyme produces small remnant peptides with still intact immunodominant epitopes
-
0
?
Galectin-3 + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
gelsolin + H2O
?
show the reaction diagram
-
-
-
0
?
heat shock protein 27 + H2O
?
show the reaction diagram
-
-
-
0
?
heat shock protein 70 + H2O
?
show the reaction diagram
-
-
-
0
?
heat shock protein 90 + H2O
?
show the reaction diagram
-
-
-
0
?
histidyl-tRNA synthetase + H2O
?
show the reaction diagram
-
-
-
0
?
HMGB1 protein + H2O
?
show the reaction diagram
-
-
-
0
?
islet amyloid polypeptide + H2O
?
show the reaction diagram
enzyme MMP-9 degrades amyloidogenic humanislet amyloid polypeptide but not nonamyloidogenic mouse islet amyloid polypeptide
-
0
?
L-Pro-L-Pro(OH)-Gly-L-Gln-Gly-L-Ala-L-Thr-Gly-L-Glu-L-Pro(OH)-Gly-NH2 + H2O
?
show the reaction diagram
-
-
-
0
?
Laminin + H2O
?
show the reaction diagram
-
-
-
0
?
LS276-THP + H2O
?
show the reaction diagram
-
development and evaluation of an activatable NIR fluorescent probe LS276-THP for in vivo detection of cancer-related matrix metalloproteinase activity based on a triplehelical peptide substrate with high specificity for MMP-2 and MMP-9 relative to other members of the MMP family, overview. Triple-helical peptides are suitable for highly specific in vivo detection of tumor-related MMP-2 and MMP-9 activity
-
0
?
Mca-KPLGL-(Dpa)-AR-NH2 + H2O
?
show the reaction diagram
-
-
0
?
moesin + H2O
?
show the reaction diagram
-
-
-
0
?
N-3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-7-methoxycoumarin-4-yl acetyl + H2O
?
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-arginine p-nitroanilide + H2O
N-alpha-benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
i.e. BAPNA
-
0
?
Neonatal human proteoglycan + H2O
?
show the reaction diagram
-
poor substrate, cleavage of the His16-Ile17 bond
-
0
?
nonmuscular actin + H2O
?
show the reaction diagram
-
-
0
?
nucleolin + H2O
?
show the reaction diagram
-
-
-
0
?
peptide A13 + H2O
?
show the reaction diagram
-
-
-
?
peptide A13R + H2O
?
show the reaction diagram
-
-
-
?
peptide A3 + H2O
?
show the reaction diagram
-
-
-
?
peptide A34 + H2O
?
show the reaction diagram
-
-
-
?
peptide B74 + H2O
?
show the reaction diagram
-
-
-
?
peptide C15 + H2O
?
show the reaction diagram
-
non-selective peptide substrate
-
?
peptide C9 + H2O
?
show the reaction diagram
-
-
-
?
peptide m1A11 + H2O
?
show the reaction diagram
-
non-selective peptide substrate
-
?
Proteoglycan + H2O
?
show the reaction diagram
-
-
-
0
?
snRNP D3 + H2O
?
show the reaction diagram
-
-
-
0
?
stathmin + H2O
?
show the reaction diagram
-
-
-
0
?
stromelysin 1 + H2O
?
show the reaction diagram
-
-
?
stromelysin1 + H2O
?
show the reaction diagram
-
-
0
?
succinylated gelatin + H2O
?
show the reaction diagram
-
-
-
0
?
tubulin + H2O
?
show the reaction diagram
-
-
-
0
?
Type IV collagen + H2O
?
show the reaction diagram
-
-
-
0
?
type V collagen + H2O
?
show the reaction diagram
-
-
-
0
?
type XI collagen + H2O
?
show the reaction diagram
-
-
-
0
?
Vitronectin + H2O
?
show the reaction diagram
-
65000 MW and 75000 MW form of vitronectin
-
0
?
vitronectin of MW 65000 + H2O
?
show the reaction diagram
-
-
-
0
?
vitronectin of MW 75000 + H2O
?
show the reaction diagram
-
-
-
0
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Aggrecan core protein + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen + H2O
?
show the reaction diagram
-
MMP-9 inhibits collagen-induced platelet aggregation
-
-
?
Collagen type I + H2O
?
show the reaction diagram
-
-
-
-
?
Collagen type III + H2O
?
show the reaction diagram
-
-
-
-
?
collagen type IV + H2O
?
show the reaction diagram
-
-
-
-
?
collagen type V + H2O
?
show the reaction diagram
-
-
-
-
?
collagen type XI + H2O
?
show the reaction diagram
-
-
-
-
?
epidermal growth factor + H2O
?
show the reaction diagram
-
MMP-9 induces TGF-beta1 production in the airway epithelium through the cleavage of epidermal growth factor, EGF, and membrane-bound EGF-like ligands and activating epidermal growth factor receptor
-
-
?
fibrillar collagen + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
islet amyloid polypeptide + H2O
?
show the reaction diagram
enzyme MMP-9 degrades amyloidogenic humanislet amyloid polypeptide but not nonamyloidogenic mouse islet amyloid polypeptide
-
-
?
Laminin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Calcium
-
required
Zinc
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2E)-3-(N-hydroxycarbamoyl)-2-(3-phenylpropylidene)propionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-heptylidenepropionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-isopropionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-3-phenylprop-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
-
-
(2E)-3-(N-hydroxycarbamoyl)-2-[(2E)-but-2-en-1-ylidene]propionyl-L-tryptophan-N-methylamide
-
-
1,10-phenanthroline
2-[(biphenyl-4-ylsulfonyl)(isobutyl)amino]-N-hydroxyacetamide
-
50% inhibition at 34 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
3',4',5,6,7,8-hexamethoxyflavone
-
i.e. nobiletin
3',4'-di-O-methylbutin-7-O-[(6''->1''')-3''',11'''-dimethyl-7'''-methylenedodeca-3''',10'''-dienyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
3',4'-di-O-methylquercetin-7-O-[(4''->13''')-2''',6''',10''',14'''-tetramethylhexadec-13'''-ol-14'''-enyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone
-
-
3'-hydroxy-4',5,6,7,8-pentamethoxyflavone
-
-
4'-hydroxy-3',5,6,7,8-pentamethoxyflavone
-
-
4'-O-methylbutin-7-O-[(6->1''')-3''',11'''-dimethyl-7'''-hydroxymethylenedodecanyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
4'-O-methylkaempferol-3-O-[(4''->13''')-2''',6''',10''',14'''-tetramethylhexadecan-13'''-olyl]-beta-D-glucopyranoside
-
0.0065-0.0259 mM suppresses MMP-9 activity in lipopolysaccharide-stimulated human monocytic cells without cytotoxicity
5-(1,5-dihydroxypentan-3-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-hydroxybutan-2-yl)-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(3-(1-nitrooxy-methyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(1-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperazin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(4-(2-nitrooxy-ethyl)piperidin-1-yl)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(bis-(2-nitrooxy-ethyl)-amino)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-(4-phenoxyphenyl)-5-(methyl-(2-nitrooxy-ethyl)-amino)-pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[3-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(2-hydroxyethyl)piperazin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(2-hydroxyethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
5-[4-(hydroxymethyl)piperidin-1-yl]-5-(4-phenoxyphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
Ag-3340
-
i.e. N-hydroxy-2,2-dimethyl-4-[(4-phenoxyphenyl)sulfonyl]thiomorpholine-3-carboxamide, 50% inhibition at 0.26 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
alpha-linolenic acid
-
antibody REGA-3G12
highly selective, potent MMP-9 inhibition, the antibody recognizes the N-terminal part, not the C-terminal hemopexin and O-glycosylated domains, epitope mapping, overview
-
carboxy-derivatized glucosamine
-
62% inhibition of MMP-9 at 0.5 mg/ml
-
CGS27023A
-
50% inhibition at 8 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
chitooligosaccharides
-
different molecular weight, inhibit both MMP-9 expression and activity, especially 1- to 3-kDa chitooligosaccharides, chitooligosaccharides-I in HT-1080 cells
D-penicillamine
-
10 mg/l, 43% inhibition
docosahexaenoic acid
-
gamma-linolenic acid
-
hemopexin domain of MMP-9
the hemopexin domain of MMP-9 inhibits the degradation of gelatin. It also abolishes the degradation of gelatin by MMP-2. Blades 1 and 4 of the PEX9 domain are responsible for the gelatin degradation inhibitory activity of PEX9. The hemopexin domain of MMP-9 does not prevent the degradation of other MMP-9 substrates, such as a fluorogenic peptide, alphaB crystalline, or nonmuscular actin
-
ilomastat
Immunoglobulins purified from antisera raised against gelatinase
-
-
-
isovitexin
interacts with enzyme residues Glu241, Ala242, Pro255, Pro254, Thr251, Leu222, Tyr248, Arg241, Met247, His226, and Glu227, binding structure analysis, and modeling, overview
linoleic acid
-
MMP-9 inhibitor I
-
N-hydroxy-2-(isobutyl[(4-methoxyphenyl)sulfonyl]amino)acetamide
-
50% inhibition at 2.9 nM, comparison with inhibitory effect on matrix metalloproteinases MMP-3, MMp-7, MMP-2
N-isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid
NNGH
norcantharidin
-
i.e. exo-7-oxabicylo-[2.2.1] heptane-2,3-dicarboxylic anhydride, norcantharidin inhibits gelatinase activity of MM-9 in a concentration- and time-dependent manner
o-phenanthroline
-
-
oleic acid
-
polyhistidine
-
0.001 mM, 68% inhibition
proanthocyanidins
-
from the american cranberry, Vaccinium macrocarpon, decrease cellular viability of DU-145 cells via inhibition of MMP-9. The proanthocyanidins PACs increase the expression of TIMP-2, a known inhibitor of MMP activity, and decrease the expression of EMMPRIN, an inducer of MMP expression. Cranberry proanthocyanidins decrease MMP activity through the induction and/or inhibition of specific temporal MMP regulators, and by affecting either the phosphorylation status and/or expression of MAP kinase, PI-3 kinase, NF-kappaB and AP-1 pathway proteins
-
procyanidin oligomers
-
from Japanese quince, Chaenomeles japonica, fruit inhibit activity of MMP-9
-
REGA-3G12 scFv with (His6)2
-
monoclonal antibody REGA-3G12 single-chain variable fragment, 0.005 mM, 66% inhibition
-
reversion-inducing cysteine-rich protein with Kazal motifs
-
-
TIMP-1
-
TIMP-1/2
-
-
-
TIMP-2
-
TIMP-4
-
-
-
TIMP1
-
interaction analysis and structure modeling, overview
-
TIMP2
-
interaction analysis and structure modeling, overview
-
Tissue inhibitor
-
tissue inhibitor of matrix metalloproteinase 1
-
strong inhibitor of MMP-9
-
tissue inhibitor of matrix metalloproteinase-1
-
TIMP-1
-
tissue inhibitor of metalloproteinase TIMP-1
-
-
tissue inhibitor of metalloproteinase TIMP-2
-
-
Tissue inhibitor of metalloproteinase-1
-
Tissue inhibitor of metalloproteinase-2
-
-
tissue inhibitor of metalloproteinases 1
-
vitexin
interacts with enzyme residues Gly186, Pro246, His236, Met247, Tyr248, Val223, and Leu188, binding structure analysis, and modeling, overview
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3,7,8-tetrachlorodibenzo-p-dioxin
-
both MMP-9 mRNA expression and enzymatic activity are gradually increased with the concentration increase of 2,3,7,8-tetrachlorodibenzo-p-dioxin in media and these changes can be reversed by resveratrol treatment in a dose-dependent manner, c-Jun mediates 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced MMP-9 expression and activity
4-amino phenylmercuric acetate
-
pro-MMP-9 is activated with 1 mM 4-amino phenylmercuric acetate in assay buffer at 37°C for 1.5 h
4-aminophenylmercuric acetate
APMA
-
-
interleukin-1
-
-
-
interleukin-1beta
-
-
-
matrix metalloproteinase 3
-
converts inactive pro-MMP-9 to active MMP-9
-
phorbol 12-myristate 13-acetate
tumor necrosis factor-alpha
-
-
-
additional information
-