Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
Ala-Leu-Ile-Leu-Thr-Leu-Val-Ser + H2O
Ala-Leu-Ile-Leu-Thr + Leu-Val-Ser
-
i.e. iPDI, competitive inhibitor of Streptococcus faecalis sex pheromones
-
?
angiotensin I + H2O
?
-
-
-
-
?
angiotensin I + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro + Phe-His-Leu
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
-
?
angiotensin II + H2O
?
-
only cleaved at Tyr-Ile
-
-
?
angiotensin II + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe
-
i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
Azoalbumin + H2O
?
-
bovine albumin, fraction V
-
-
?
azocasein + H2O
?
-
-
-
-
?
beta-Lipotropin
?
-
cleavage site: Gly63-Phe64, poor substrate
-
-
?
Big endothelin-1 fragment(22-38) + H2O
Hydrolyzed fragment of big endothelin fragment
Bovine casein-yellow + H2O
?
-
-
-
-
?
Bovine fibrinogen + H2O
?
Bovine hemoglobin + H2O
?
-
-
-
-
?
Bovine pancreas insulin + H2O
?
-
poor substrate
-
-
?
bradykinin + H2O
?
-
-
-
-
?
Cholecystokinin + H2O
?
-
cleavage site: Gly29-Trp30
-
-
?
clots milk + H2O
?
-
-
-
-
?
Collagen + H2O
?
-
high or low viscose polypetides from collagen
-
-
?
Gelatin + H2O
?
-
-
-
-
?
Hemoglobin + H2O
?
-
-
-
-
?
human endothelin 1 + H2O
?
-
-
-
-
?
Human endothelin-1 + H2O
?
Insulin A-chain + H2O
Fragments of insulin A-chain
-
cleavage at Gln15-Leu16 and Tyr14-Gln15, to a much lesser extent than B-chain
peptides (Gln1-Gln15) and (Leu16-Asn21), another product formed: peptide (Gln1-Trp14)
?
insulin B chain + H2O
?
-
rapidly hydrolyzed at Phe24-Phe25, followed by the cleavage of the His5-Leu6 and other bonds
-
-
?
Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly + H2O
Leu-Phe-Ser + Leu-Val-Leu-Ala-Gly
-
i.e. cADI, Streptococcus faecalis sex pheromone, poor substrate
-
?
Leu-Phe-Val-Val-Thr-Leu-Val-Gly + H2O
Leu-Phe-Val-Val-Thr + Leu-Val-Gly
-
i.e. iADI, competitive inhibitor of Streptococcus faecalis sex pheromones
-
?
Leucine-enkephalin + H2O
?
-
cleavage site: Gly3-Phe4, poor substrate
-
-
?
Lys-bradykinin + H2O
?
-
-
-
-
?
N-3-(2-Furyl)acryloyl tripeptides derivatives + H2O
?
-
chromogenic substrates, e.g. -L-Ala-L-Ala-L-Ala, -Gly-L-Phe-L-Phe, -L-Phe-L-Phe-L-Phe, -Gly-L-Leu-L-Tyr, -Gly-L-Met-L-Leu, -L-Leu-L-Leu-L-Leu
-
-
?
N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O
?
N-3-(2-Furyl)acryloyl-Gly-L-Phe + H2O
?
-
chromogenic substrate
-
-
?
neurotensin + H2O
fragments of neurotensin
-
cleavage site: Tyr11-Ile12, poor substrate
peptides (Arg1-Gly9) and (Leu10-Met11) are the first occuring products
?
Phe-Leu-Val-Met-Phe-Leu-Ser-Gly + H2O
Phe-Leu-Val-Met-Phe + Leu-Ser-Gly
-
i.e. cPDI, Streptococcus faecalis sex pheromone, poor substrate
-
?
pheromone related peptide + H2O
?
-
-
-
-
?
Substance P + H2O
?
-
-
-
-
?
Substance P + H2O
Arg-Pro-Lys-Pro-Gln-Gln + Phe + Phe-Gly + Leu-Met-NH2
-
i.e. Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
-
?
succinyl-Ala-Glu-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
succinyl-Val-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
[Lys1]bradykinin + H2O
?
-
-
-
-
?
additional information
?
-
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
?
-
-
?
3-methoxysuccinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
?
-
-
?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
acetyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
-
-
?
Azocoll + H2O
?
Enterobacter faecalis
-
general protease substrate
-
-
?
Azocoll + H2O
?
Enterobacter faecalis OG1-10
-
general protease substrate
-
-
?
Azocoll + H2O
?
-
-
-
-
?
Azocoll + H2O
?
-
general protease substrate
-
-
?
beta-casein + H2O
?
-
-
?
beta-casein + H2O
?
-
-
?
Big endothelin-1 + H2O
?
Enterobacter faecalis
-
human, cleavage sites: Ser5-Leu6 and His16-Leu17
-
-
?
Big endothelin-1 + H2O
?
Enterobacter faecalis OG1-10
-
human, cleavage sites: Ser5-Leu6 and His16-Leu17
-
-
?
Big endothelin-1 fragment(22-38) + H2O
Hydrolyzed fragment of big endothelin fragment
Enterobacter faecalis
-
-
peptides (Val22-Val28) and (Val29-Gly32) and (Leu33-Ser38)
?
Big endothelin-1 fragment(22-38) + H2O
Hydrolyzed fragment of big endothelin fragment
Enterobacter faecalis OG1-10
-
-
peptides (Val22-Val28) and (Val29-Gly32) and (Leu33-Ser38)
?
Bovine fibrinogen + H2O
?
-
B-chain and to a lesser extent A-chain
-
-
?
Bovine fibrinogen + H2O
?
-
plasminogen-free or fraction I
-
-
?
casein + H2O
?
-
-
-
-
?
Endothelin 1 + H2O
?
Enterobacter faecalis
-
presumably involved in initiation and propagation of Enterobacter faecalis associated inflammations, destroys biological activity of endothelin 1
-
-
?
Endothelin 1 + H2O
?
Enterobacter faecalis OG1-10
-
presumably involved in initiation and propagation of Enterobacter faecalis associated inflammations, destroys biological activity of endothelin 1
-
-
?
Glucagon + H2O
?
-
cleavage site: Trp25-Leu26
-
-
?
Glucagon + H2O
?
-
-
-
-
?
Human endothelin-1 + H2O
?
Enterobacter faecalis
-
primary cleavage at Ser5-Leu6, subsequent hydrolysis of His16-Leu17, degradation resembles processing by thermolysin, identification of products
-
-
?
Human endothelin-1 + H2O
?
Enterobacter faecalis OG1-10
-
primary cleavage at Ser5-Leu6, subsequent hydrolysis of His16-Leu17, degradation resembles processing by thermolysin, identification of products
-
-
?
Insulin B-chain + H2O
?
-
oxidized, best substrate, preferred cleavage at Phe24-Phe25, followed by His5-Leu6, after prolonged incubation: Ala14-Leu15 and His10-Leu11
-
-
?
Insulin B-chain + H2O
?
-
-
-
-
?
N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O
?
-
-
-
-
?
N-3-(2-Furyl)acryloyl-Gly-L-Leu amide + H2O
?
-
chromogenic substrate, cleavage site: Gly-Leu, poor substrate
-
-
?
additional information
?
-
-
strict specificity for hydrophobic amino acid at position P1', exhibits endopeptidase and thermolysin-like specificity
-
-
?
additional information
?
-
-
very poor substrates are elastin-orcein or hide powder azure
-
-
?
additional information
?
-
-
No substrates are Ala-Ile-Phe-Ile-Ala-Ser (i.e. Streptococcus faecalis sex pheromone cAM373), aminoacyl-2-naphthylamides, Nalpha-benzoyl-DL-Arg 4-nitroanilide, phenylazobenzyloxycarbonyl-L-Pro-L-Leu-Gly-L-Pro-D-Arg, 2-furylacryloyl-L-Leu-Gly-L-Pro-L-Ala
-
-
?
additional information
?
-
-
No substrates are N-3-(2-furyl)acryloyl-Gly-Gly-Gly, N-3-(2-furyl)acryloyl-Gly-L-Leu or N-3-(2-furyl)acryloyl-Gly-L-Ala
-
-
?
additional information
?
-
-
neutral metalloendopeptidase, no aminopeptidase or esterase activity
-
-
?
additional information
?
-
-
no cleavage of Pz-Pro-Leu-Gly-Pro-D-Arg and 2-FA-Leu-Gly-Pro-Ala
-
-
?
additional information
?
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
additional information
?
-
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
additional information
?
-
Bz-Phe-Val-Arg-p-nitroanilide, NZbz-Gly-Pro-Arg-p-nitroanilide, and Z-Phe-Arg-p-nitroanilide are no substrates
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,4,5,6-Tetrachloro-1,2-benzoquinone
-
i.e. o-chloranil, 0.003 mM, most potent inactivator
chymostatin
75% inhibition
diethylpyrocarbonate
-
partially reversed by hydroxylamine (not)
high molecular mass inflammatory factor
-
-
-
High MW factor of rat inflammatory exudate
-
strong, MW: 720000-750000
-
iodoacetic acid
83% inhibition
L-Leucine hydroxamate
-
-
metal chelator
-
inactivates
-
methanol
-
about 15% as effective as n-butanol
N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline
-
5 mM
NaBr
-
above 5 M, activates at 1-5 M
o-Chloranil
-
is a near-stoichiometric inhibitor of the enzyme
phenylmethylsulfonyl fluoride
strongly inhibited, 100% inhibition
phosphoramidon
-
i.e. N-(alpha-L-rhamnopyranosyloxy-hydroxyphosphinyl)-L-Leu-L-Trp
Tetranitromethane
-
5 mM, pH-dependent
1,10-phenanthroline
-
-
EDTA
-
reversible by Co2+, Mn2+, Zn2+, Ca2+, Cu2+ or Mg2+ with decreasing order of efficiency, not by Cd2+, Ba2+, Ni2+ or Hg2+
p-chloromercuribenzoate
-
not
p-chloromercuribenzoate
-
-
additional information
-
no inhibition by DTNB, carbodiimide/glycine ethyl ester; no inhibition by iodoacetate; no inhibition by PMSF, phenylglyoxal; no inhibition by tosyl-Leu chloromethyl ketone, tosyl-Phe chloromethyl ketone, trasylol (i.e. bovine lung trypsin inhibitor)
-
additional information
-
no inhibition by 2,3-butanedinone; no inhibition by PMSF, phenylglyoxal
-
additional information
-
diisopropyl fluorophosphate; no inhibition by iodoacetate
-
additional information
no inhibition with bestatin or E64
-
additional information
-
no inhibition with bestatin or E64
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Encephalomyelitis, Autoimmune, Experimental
Treatment of an encephalitogenic peptide from guinea pig myelin basic protein with alpha-protease and thermolysin. Isolation of fragments and determination of cleavage sites.
Infections
Endemic acinetobacter in intensive care units: epidemiology and clinical impact.
Infections
Glycosylation of a Streptomyces coelicolor A3(2) cell envelope protein is required for infection by bacteriophage phi C31.
Meningitis
Homogeneity of cell envelope protein subtypes, lipopolysaccharide serotypes, and biotypes among Haemophilus influenzae type b from patients with meningitis in The Netherlands.
Muscular Dystrophies
Therapeutic trials in muscular dystrophy. III. Studies of microbial proteinase inhibitors in murine dystrophy.
Neoplasms
Synthesis and HPLC analysis of enzymatically cleavable linker consisting of poly(ethylene glycol) and dipeptide for the development of immunoconjugate.
Pancreatitis
Acute hemorrhagic pancreatic necrosis in mice. Effects of proteinase inhibitors on its induction.
Peptic Ulcer
Helicobacter pylori in pediatrics.
Periodontitis
Isolation and characterization of a 53 kDa major cell envelope protein antigen from Treponema denticola ATCC 35405.
Tuberculosis
Cell envelope protein PPE68 contributes to Mycobacterium tuberculosis RD1 immunogenicity independently of a 10-kilodalton culture filtrate protein and ESAT-6.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Smith, R.A.G.; Green, J.; Kopper, P.H.
The purification and properties of a fibrinolytic neutral metalloendopeptidase from Streptococcus faecalis
Arch. Biochem. Biophys.
202
629-638
1980
Enterococcus faecalis
brenda
Mäkinen, P.L.; Clewell, D.B.; An, F.; Mäkinen, K.K.
Purification and substrate specificity of a strongly hydrophobic extracellular metalloendopeptidase (gelatinase) from Streptococcus faecalis (strain 0G1-10)
J. Biol. Chem.
264
3325-3334
1989
Enterococcus faecalis
brenda
Mäkinen, P.L.; Mäkinen, K.K.
The Enterococcus faecalis extracellular metalloendopeptidase (EC 3.4.24.30; coccolysin) inactivates human endothelin at bonds involving hydrophobic amino acid residues
Biochem. Biophys. Res. Commun.
200
981-985
1994
Enterobacter faecalis, Enterobacter faecalis OG1-10
brenda
Desmazeaud, M.J.
General properties and specificity of action of a neutral intracellular endopeptidase from Streptococcus thermophilus
Biochimie
56
1173-1181
1974
Streptococcus thermophilus
brenda
Fernandez-Espla, M.D.; Garault, P.; Monnet, V.; Rul, F.
Streptococcus thermophilus cell wall-anchored proteinase: release, purification, and biochemical and genetic characterization
Appl. Environ. Microbiol.
66
4772-4778
2000
Streptococcus thermophilus (Q9F8Q4), Streptococcus thermophilus, Streptococcus thermophilus CNRZ 385 (Q9F8Q4)
brenda
Makinen, K.K.; Makinen, P.
Coccolysin
Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press
1
396-398
2004
Enterococcus faecalis
-
brenda
Domann, E.; Hain, T.; Ghai, R.; Billion, A.; Kuenne, C.; Zimmermann, K.; Chakraborty, T.
Comparative genomic analysis for the presence of potential enterococcal virulence factors in the probiotic Enterococcus faecalis strain Symbioflor 1
Int. J. Med. Microbiol.
297
533-539
2007
no activity in Enterococcus faecalis, no activity in Enterococcus faecalis Symbioflor 1
brenda